Impact of FilmArray™ Respiratory Panel testing on the clinical management of pediatric bone marrow transplant patients

Precit, Mimi R.; He, Kaidi; Mongkolrattanothai, Kanokporn; Moeini, Babak; Glucoft, Marisa; Bard, Jennifer Dien

doi:10.1007/s10096-021-04382-4

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…For instance, human coronavirus NL63 and 229E are detected using the nucleocapsid ( N ) gene; influenza A targets the matrix ( M ) gene, while influenza B targets the non-structural ( NS ) gene; human adenovirus targets the hexon gene; parainfluenza viruses 1–4 target the hemagglutinin-neuraminidase ( HN ) gene; both human coronavirus OC43 and RSV are identified through the N gene; human metapneumovirus targets the matrix ( M ) gene; coronavirus HKU1 targets the N gene; human rhinovirus/enterovirus is detected through the 5′ non-coding region (5′NCR); Middle East respiratory syndrome coronavirus utilizes the upstream of the E protein (UpE) and ORF1b genes; and SARS-CoV-2 often targets the ORF1ab , N , and spike ( S ) genes. In the PCR process of the BioFire respiratory panel 2.1, these targeted genes, represented by specific primers, are mixed in a single reaction, and their amplification signifies the presence of the respective pathogen in the sample [ 24 ]. It allows for the rapid detection of the causative virus [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of Respiratory Viruses and SARS-CoV-2 on Febrile Seizures in Saudi Children: Insights into Etiologies, Gender, and Familial Associations

AlFulayyih,

Al Baridi,

Alomar

et al. 2023

Med Sci Monit

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Background Childhood febrile seizures occur between 5 months and 6 years of age in children without a previous history of seizure and are associated with high temperature in the absence of intracranial infection. This retrospective study identified 71 children aged 6 months to 5 years with febrile seizures between 2017 and 2021 at a single center in Saudi Arabia and aimed to identify an association between common respiratory virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Material/Methods Pediatric nasopharyngeal specimens were tested using a multiplex PCR respiratory panel detecting human coronaviruses (NL63, 229E, OC43, HKU1), influenza A/B, human adenovirus, parainfluenza viruses 1–4, respiratory syncytial virus, human metapneumovirus, rhinovirus/enterovirus, Middle East respiratory syndrome coronavirus, and, as of September 2021, SARS-CoV-2, confirmed using the Cepheid Xpert Xpress SARS-CoV2 RT-PCR kit. Results In a cohort of 71 pediatric patients (median age, 19 months; 54.9% female), dominant pathogens included human rhinovirus/enterovirus (23.9%), influenza A/B (26.8%), and SARS-CoV-2 (14.1%). Concurrent infections were noted in 28.2%. Simple seizures occurred in 69%, and complex seizures in 31%. Females exhibited an 8.18-fold increased risk for complex seizures. Each additional fever day reduced complex seizure risk by 36%. Familial seizure history increased risk 8.76-fold. Human rhinovirus/enterovirus or parainfluenza infections inversely affected complex seizure likelihood compared with adenovirus. Conclusions In Saudi children with febrile seizures, distinct viral etiologies, sex, and familial links play pivotal roles. Given regional viral variations, region-tailored diagnostic and therapeutic strategies are paramount. A multicenter prospective cohort study is essential for comprehensive understanding.

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Section: Methodsmentioning

confidence: 99%

Impact of Respiratory Viruses and SARS-CoV-2 on Febrile Seizures in Saudi Children: Insights into Etiologies, Gender, and Familial Associations

AlFulayyih,

Al Baridi,

Alomar

et al. 2023

Med Sci Monit

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“…Of the 402 samples from patients with upper respiratory tract symptoms, 54% tested positive for a respiratory virus. Results from the rapid test also informed infection control measures for all inpatients and antiviral initiation in >80% of episodes with influenza [8].…”

Section: Standard Diagnosticsmentioning

confidence: 99%

“…Methods for rapid detection of viral respiratory infections have improved in recent times. The film array respiratory panel (FA-RP) detects 17 viral respiratory pathogens and the clinical utility was retrospectively analysed in 671 nasal swabs from 204 paediatric HSCT patients [8]. Of the 402 samples from patients with upper respiratory tract symptoms, 54% tested positive for a respiratory virus.…”

Section: Nasal Swabmentioning

confidence: 99%

Novel approaches to the prediction and diagnosis of pulmonary complications in the paediatric haematopoietic stem cell transplant patient

Walker

Shanthikumar

Cole

et al. 2022

Current Opinion in Infectious Diseases

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Purpose of reviewHaematopoietic stem cell transplant (HSCT) remains the only curative treatment option for many children with relapsed leukaemia, primary immunodeficiencies and haemoglobinopathies. Unfortunately, infectious and noninfectious pulmonary complications following HSCT continue to cause significant morbidity and mortality. This review will focus on recent advances in the field that enhance clinically available diagnostic tools and the role of novel diagnostic techniques.Recent findingsResearch continues to highlight the role of standard diagnostic modalities, including imaging using computed topography chest and Fluorodeoxyglucose-positron emission tomography (FDG-PET) in the diagnosis of posttransplant pulmonary infections. Similarly, bronchoalveolar lavage using bronchoscopy to obtain samples for microbiological analysis remains an important tool in the clinical and diagnostic algorithm for these children. The application of more novel diagnostic techniques such as metagenomic next-generation sequencing and the use of specific biomarkers remain potential future tools in children in whom the aetiology of posttransplant lung disease is unknown. The impact of the pulmonary microbiome on infectious and noninfectious pulmonary disease post HSCT is a future research direction.SummaryPulmonary infectious complications post HSCT remain a devastating complication for children and their families. Despite improvements in standard and novel diagnostic modalities, the aetiology of pulmonary disease remains unknown for many patients. There is an urgent need for ongoing collaborative research to bridge this critical knowledge gap and lead to better patient outcomes.

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“…[10][11][12] Studies evaluating clinical outcomes for children positive for HRV/ENT on RM-PCR testing remain limited, with analyses focusing on inpatient or clinical outcomes in specific populations. [13][14][15][16][17] A 2014 analysis of inpatient clinical measures demonstrated that children with HRV/ENT positivity on RM-PCR were likelier to be hospitalized and have more severe illnesses than those positive for other viruses. 18 An outpatient analysis evaluating the clinical and socioeconomic burden of HRV/ ENT positivity in adults and children revealed that under-17-yearolds positive for HRV/ENT on RM-PCR were likelier than adults to seek medical care.…”

mentioning

confidence: 99%

Comparing the Clinical Courses of Children With Human Rhinovirus/Enterovirus to Children With Other Respiratory Viruses in the Outpatient Setting

Jones,

Montgomery,

Coskun

et al. 2023

Pediatric Infectious Disease Journal

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Background: While infections caused by rhinoviruses and enteroviruses are common among children, the entirety of their clinical impact remains elusive. We compared the clinical outcomes of children with rhinovirus/enterovirus infections to other common respiratory viruses in outpatient settings. Methods: We conducted a retrospective analysis of nasopharyngeal samples singly positive for human rhinovirus/enterovirus (HRV/ENT), influenza A/B (FLU) or respiratory syncytial virus (RSV) from patients ≤17 years submitted for clinical testing via multiplex polymerase chain reaction between 2016 and 2019. We evaluated the following outpatient outcomes: days of respiratory symptoms before testing; visits for respiratory symptoms; receipt of a breathing treatment; receipt of antibiotics and hospital admission. Statistical analyses were conducted controlling for age and comorbid conditions. Results: There were 1355 positive samples included in this analysis (HRV/ENT: n = 743, FLU: n = 303 and RSV: n = 309). Compared to HRV/ENT, children with FLU had 28% fewer days of respiratory symptoms (β: −0.32; 95% confidence interval: −0.46 to −0.18; P < 0.001), fewer visits for respiratory symptoms, and significantly decreased odds of receiving a breathing treatment or antibiotics, and admission to the hospital. Children with RSV had a similar number of days of respiratory symptoms, outpatient visits and odds of hospital admission, but significantly increased odds of receiving a breathing treatment and antibiotics compared to those with HRV/ENT. Conclusion: Clinicians should have a high level of vigilance when managing children with positive respiratory viral testing for HRV/ENT given the potential for clinical outcomes similar to and, in some instances, worse than known highly pathogenic viruses.

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Impact of FilmArray™ Respiratory Panel testing on the clinical management of pediatric bone marrow transplant patients

Cited by 4 publications

References 19 publications

Impact of Respiratory Viruses and SARS-CoV-2 on Febrile Seizures in Saudi Children: Insights into Etiologies, Gender, and Familial Associations

Impact of Respiratory Viruses and SARS-CoV-2 on Febrile Seizures in Saudi Children: Insights into Etiologies, Gender, and Familial Associations

Novel approaches to the prediction and diagnosis of pulmonary complications in the paediatric haematopoietic stem cell transplant patient

Comparing the Clinical Courses of Children With Human Rhinovirus/Enterovirus to Children With Other Respiratory Viruses in the Outpatient Setting

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