Impact of fiber types on gut microbiota, gut environment and gut function in fattening pigs

Chen, H.; Mao, Xiangbing; Che, Lianqiang; Yu, Bing; He, Jun; Yu, Jie; Han, Guangting; Huang, Zhiqing; Zheng, Ping; Chen, D.W.

doi:10.1016/j.anifeedsci.2014.06.002

Cited by 63 publications

(58 citation statements)

References 40 publications

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“…For example, longer villi and shorter crypts enhance absorptive capacity (55)(56)(57). However, while we found no differences in these parameters between pigs of varying FE, we did find fewer goblet cells along the villi of pigs that were more feed efficient.…”

Section: Discussioncontrasting

confidence: 53%

Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs

McCormack

Curião

Buzoianu

et al. 2017

Appl Environ Microbiol

264

208

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Feed efficiency (FE) is critical in pig production for both economic and environmental reasons. As the intestinal microbiota plays an important role in energy harvest, it is likely to influence FE. Therefore, our aim was to characterize the intestinal microbiota of pigs ranked as low, medium, and high residual feed intake ([RFI] a metric for FE), where genetic, nutritional, and management effects were minimized, to explore a possible link between the intestinal microbiota and FE. Eightyone pigs were ranked according to RFI between weaning and day 126 postweaning, and 32 were selected as the extremes in RFI (12 low, 10 medium, and 10 high). Intestinal microbiota diversity, composition, and predicted functionality were assessed by 16S rRNA gene sequencing. Although no differences in microbial diversity were found, some RFI-associated compositional differences were revealed, principally among members of Firmicutes, predominantly in feces at slaughter (albeit mainly for low-abundance taxa). In particular, microbes associated with a leaner and healthier host (e.g., Christensenellaceae, Oscillibacter, and Cellulosilyticum) were enriched in low RFI (more feed-efficient) pigs. Differences were also observed in the ileum of low RFI pigs; most notably, Nocardiaceae (Rhodococcus) were less abundant. Predictive functional analysis suggested improved metabolic capabilities in these animals, especially within the ileal microbiota. Higher ileal isobutyric acid concentrations were also found in low RFI pigs. Overall, the differences observed within the intestinal microbiota of low RFI pigs compared with that of their high RFI counterparts, albeit relatively subtle, suggest a possible link between the intestinal microbiota and FE in pigs.IMPORTANCE This study is one of the first to show that differences in intestinal microbiota composition, albeit subtle, may partly explain improved feed efficiency (FE) in low residual feed intake (RFI) pigs. One of the main findings is that, although microbial diversity did not differ among animals of varying FE, specific intestinal microbes could potentially be linked with porcine FE. However, as the factors impacting FE are still not fully understood, intestinal microbiota composition may not be a major factor determining differences in FE. Nonetheless, this work has provided a potential set of microbial biomarkers for FE in pigs. Although culturability could be a limiting factor and intervention studies are required, these taxa could potentially be targeted in the future to manipulate the intestinal microbiome so as to improve

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Section: Discussioncontrasting

confidence: 53%

Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs

McCormack

Curião

Buzoianu

et al. 2017

Appl Environ Microbiol

264

208

View full text Add to dashboard Cite

show abstract

“…are related with hemicellulose fermentation process. Chen [22] illuminated that different fiber sources resulted in inconsistent microbiota composition in pigs gut. As expected, a number of the predicted functions of these potential biomarkers in H-NDF group and H-ADF group were associated with microbial cell metabolism.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota Related to Fiber Digestibility were Identified by Variation of Apparent Fiber Digestibility in Chinese Suhuai pig

Niu

Fan

et al. 2020

Preprint

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Background: Dietary fiber plays an important role in porcine gut health and welfare. Fiber mainly degraded by the gut microbiota, whereas most gut microbiota related to fiber digestibility of pigs are still unidentified. To reveal gut microbiota associated with apparent digestibility of neutral detergent fiber (NDF) and acid detergent fiber (ADF), apparent NDF, ADF digestibility of 274 Suhuai female finishing pigs at the age of 160 days were measured. The gut microbiota of Suhuai pigs were analyzed through 16S rRNA gene sequencing, respectively. Results: Large phenotypic variations in apparent NDF and ADF digestibility were separately found among Suhuai pigs. The coefficient of variation of NDF and ADF digestibility was 12.08% and 18.08%, respectively. The mean values of digestibility of H-NDF and H-ADF groups were 30.20% and 33.76% more than those of the L-NDF and L-ADF groups (P＜0.01), respectively. A total of 927 and 935 operational taxonomic units (OTUs) were confirmed from two types of fecal samples,respectively. There were14 phyla in all samples and the abundances of Spirochaetae, Bacteroidetes and unclassified_k__norank were significantly different between H-NDF and L-NDF groups (P＜0.05) and the abundances of Spirochaetae, Verrucomicrobia, unclassified_k__norank and Fibrobactere were significantly different between H-ADF and L-ADF group (P＜0.05).A total of 188, 183, 188 and 185 genera were separately identified in H-NDF, L-NDF, H-ADF and L-ADF groups, while 6, 1, 5 and 2 genera were separately specific to H-NDF, L-NDF, H-ADF and L-ADF groups. The microbiota of H-NDF and H-ADF clustered separately from the microbiota of the L-NDF and L-ADF along principal coordinate 1, respectively. Compared with L-NDF group, 29 genera were found to be potential biomarkers in H-NDF group. Compared with L-ADF group, 23 genera were found to be potential biomarkers in H-ADF group. The most important functions and metabolic pathways of the above potential biomarkers included carbohydrate transport and metabolism. Conclusions: Microbial community structures were significantly different between high and low fiber digestibility groups. Twenty nine and 23 genera were found to be potential biomarkers in H-NDF and H-ADF group, respectively. The biomarkers may be the key functional microbiota related to apparent fiber digestibility.

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Section: Discussionmentioning

confidence: 99%

“…The butyrate-type fermentation of cereal fibre is attributed to its arabinoxylans (Chen et al, 2014;Ivarsson et al, 2014). The acetate-type fermentation of legume fibre is linked to polyuronides (Jonathan et al, 2012;Chen et al, 2014;Ivarsson et al, 2014). Intense fermentation with substantial BUT shift for maize and its hominy chop could be attributed to a concentration of resistant starch (Jonathan et al, 2012;Giuberti et al, 2013;He et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Co-products in maize-soybean growing-pig diets altered <i>in vitro</i> enzymatic insoluble fibre hydrolysis and fermentation in relation to botanical origin

Fushai¹,

Tekere²,

Masafu³

et al. 2019

SA J. An. Sci.

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The study examined the effects of botanical factors and fermentation-based, high-level dilution of coproduct feeds in maize-soybean growing-pig diets on enzymatic insoluble fibre hydrolysis and fermentation. Feed insoluble fibre residues that were recovered after pepsin-pancreatin digestion were subjected to Roxazyme ® G2 (Roxazyme) versus Viscozyme L ® V2010 (control) hydrolysis, and to 64-hour fermentation using pig faecal inoculum. The control diet was a 13 MJ metabolizable energy, 141 g total dietary fibre/kg dry matter maize-meal/hominy chop-soybean diet, which was diluted with maize cob, soybean hulls, barley brewer's grains, lucerne hay or wheat bran in 12 MJ metabolizable energy, 246 g total dietary fibre/kg dry matter iso-nutrient, single co-product test diets. Fermentable insoluble fibre was employed in a computerized iterative selection of ingredients in two iso-nutrient 11 MJ metabolizable energy 319 total dietary fibre/kg dry matter mixed fibre test diets for maximal contrast (high (HF) versus low (LF)) in fermentability. Insoluble fibre extractive pepsin-pancreatin digestibility differed between feed ingredients, and the single co-product test diets, and between the HF and LF mixed co-product diets. Fibre digestibility depended on both the origin and enzyme, with interaction, whereby carbohydrases expressed similar low (0.04-0.05) insoluble fibre digestibility for maize cob, moderate (0.12) digestibility for wheat bran and brewer's grain, with inferior Roxazyme G2 digestibility for maize hominy chop (0.02 vs 0.10) and meal (0.04 vs 0.16), dehulled soybean meal (0.02 vs 0.17), lucerne hay (0.08 vs 0.18), and soybean hulls (0.05 vs 0.33). Co-product-enzyme affinities were expressed in single fibre diets. Low Roxazyme-basal fibre affinity limited its comparative single co-product (0.03-0.07 vs 0.16-0.22) HF (0.07 vs 0.17) and LF (0.4 vs 0.20) dietary fibre digestibility. Screening for HF/LF did not affect enzymatic digestion, though enzyme combination increased HF, but not LF digestibility. Gas and short chain fatty acid production predicted fermentability proportionately in the declining order of dehulled soybean ≥ maize ≥ soy hulls ≥ maize hominy chop > wheat bran >lucerne hay ≥ brewer's grain = maize cob. Induced HF and LF contrast was significant. Co-product fibre enrichment decreased fermentability for all except the soy hull and HF diets. Cereal fibre yielded proportionately less acetate, with more propionate and butyrate, and a greater butyrate shift for maize fibre. The HF fibre induced more ACE and less butyrate. Biomarkers of deleterious proteolytic fermentation were high for lucerne (isobutyrate) and soy hulls (iso-valerate). In conclusion, high-level and fermentation based co-product feed dilution into maize-soybean growing pig diets altered enzymatic insoluble fibre hydrolysis and fermentation in relation to botanical origin. Roxazyme expressed weak hydrolytic potency on maize and soybean insoluble fibre.

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Impact of fiber types on gut microbiota, gut environment and gut function in fattening pigs

Cited by 63 publications

References 40 publications

Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs

Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs

Gut Microbiota Related to Fiber Digestibility were Identified by Variation of Apparent Fiber Digestibility in Chinese Suhuai pig

Co-products in maize-soybean growing-pig diets altered <i>in vitro</i> enzymatic insoluble fibre hydrolysis and fermentation in relation to botanical origin

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