Impact of fabrics from transgenic flax on cultures of skin cells

Gąsiorowski, Kazimierz; Gębarowski, Tomasz; Moreira, Helena; Kulma, Anna; Szatkowski, Michał; Szopa, Jan

doi:10.17219/acem/92563

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Thus, synthesis of PHB may have a positive effect on the proliferation of plant cells. The similar effect of fibres containing PHB on human cells (keratinocytes and fibroblasts) was noticed and described (Gąsiorowski et al 2019). Thus, further experiments that focus on elicitor supplementation to callus suspension cultures will be done in the nearest future and the impact of PHB on plant cell proliferation will be assessed.…”

Section: Discussionmentioning

confidence: 57%

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Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB

Wróbel‐Kwiatkowska

Kostyń

Dymińska

et al. 2020

Plant Cell Tiss Organ Cult

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Linum usitatissimum L. is a plant used by human since ancient times. Presently flax has both industrial and nutritional significance. The programmes of restoration of flax cultivation and processing are implemented to renew the importance of this plant for agriculture and economy. Genetic engineering methods and techniques of plant tissue cultures can answer this requirement, because they give the opportunity for obtaining of new plants and novel application of them. In the present study the impact of genetic modification of flax plants on the ability of morphogenesis was investigated. The subject of this research remain genetically modified flax plants enriched in poly-β-hydroxybutyrate (PHB), interesting biodegradable compound showing bioactive properties and acting as anti-inflammatory and anti-microbial factor. Another aim of this study was to characterize chemical and structural properties of callus derived from the engineered plants in comparison to the data obtained for non-transgenic, control callus cultures. The presence of PHB was confirmed by FTIR technique and it was suggested that this polymer may have impact on about 20% higher biomass growth observed for transgenic callus cultures in comparison to control cultures. The level of phenolic compounds was not significantly changed in the transgenic calluses when compared to wild-type cultures. The extracellular matrix (EMC) was noticed for the surface of genetically modified calluses, such structure was not visible for calluses derived from the control cultures. The cellulose in plant cell walls of transgenic calluses was structurally changed, it was not as organized as in control callus, resulting in lower crystallinity index values. Key message 1. FTIR and SEM analyses revealed differences between callus producing PHB when compared to wild-type callus. 2. PHB might influence the proliferative properties of plant cells.

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Section: Discussionmentioning

confidence: 57%

“…One of the transgenic lines with the highest PHB level (M50) was used as the source of fibres for composite preparation (Wróbel-Kwiatkowska et al 2012) and the basis for micronization (Dymińska et al 2012). Fibres isolated from the modified M plants were also applied as promising biomaterial for wound healing (Gąsiorowski et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB

Wróbel‐Kwiatkowska

Kostyń

Dymińska

et al. 2020

Plant Cell Tiss Organ Cult

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Investigation of the Properties of Linen Fibers and Dressings

Gębarowski

Jęśkowiak

Wiatrak

2022

IJMS

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In antiquity, flax was used as a dressing for healing wounds. Currently, work is underway on the genetic modification of flax fibers to improve their properties. Genetic modifications have resulted in an increased content of antioxidants and more favorable mechanical properties. The works published so far have presented independent tests of fibers and dressings after appropriate technological treatments in cell cultures. This study aimed to compare the properties of the fibers and the dressing produced in cell cultures—hamster fibroblasts—V79. The research material was traditional NIKE fibers; genetically modified M, B, and MB fibers; and linen dressings obtained from these fibers. The extract from 48-h incubation of 40 mg of fiber in the culture medium, which was desolved into 10, 20, and 30 mg, was administered to the cell culture. On the other hand, a linen dressing was placed on cells with an area of 0.5 cm2, 1 cm2, 1.5 cm2, and 2 cm2. Cells with fiber or dressing were incubated for 48 h, and then, biological tests were performed, including cell viability (in propidium iodide staining), cell proliferation (in the SRB assay), evaluation of the intracellular free radical level (in the DCF-DA assay), genotoxicity (in the comet assay), assessment of the apoptotic and necrotic cells (in staining anexin-V and iodide propidium), the course of the cell cycle, and the scratch test. The correlation between apoptosis and genotoxicity and the levels of free radicals and genotoxicity were determined for the tested linen fibers and fabrics. The tests presented that the fibers are characterized by the ability to eliminate damaged cells in the elimination phase. However, the obtained fabrics gain different properties during the technological processing of the fibers into linen dressings. Linen fabrics have better regenerative properties for cells than fibers. The linseed dressing made of MB fiber has the most favorable regenerative properties.

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Impact of fabrics from transgenic flax on cultures of skin cells

Cited by 2 publications

References 21 publications

Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB

Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB

Investigation of the Properties of Linen Fibers and Dressings

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