Impact of Drying Methods on Phenolic Components and Antioxidant Activity of Sea Buckthorn (Hippophae rhamnoides L.) Berries from Different Varieties in China

Abstract

Sea buckthorn berries are rich in bioactive compounds and can be used for medicine and food. The variety and drying method used have an important influence on quality. In this study, different sea buckthorn varieties from China were selected and dried with four common drying methods. The total phenolic content (TPC), total flavonoids content (TFC), contents of 12 phenolic compounds and antioxidant capacity in vitro were analyzed. The results showed that the TPC, TFC and antioxidant activity of two wild sea buckthorn berries were higher than those of three cultivated berries, and for the same varieties, measured chemical contents and antioxidant activity of the freeze-dried fruit were significantly higher than those obtained with three conventional drying methods. In addition, forty-one compounds in sea buckthorn berry were identified by UPLC-PDA-Q/TOF-MS, most of which were isorhamnetin derivatives. Multivariate statistical analysis revealed narcissin and isorhamnetin-3-O-glucoside varied significantly in sea buckthorn berries of different varieties and with different drying methods; they were potential quality markers. Strong correlations were found between TPC, gallic acid and antioxidant capacity (p < 0.05). The results revealed how components and antioxidant activity varied in different sea buckthorn, which provides a valuable reference for quality control and further development and utilization of sea buckthorn.

Reclaim and Valorization of Sea Buckthorn (Hippophae rhamnoides) By-Product: Antioxidant Activity and Chemical Characterization

“…Regarding the total flavonoid content expressed in rutin, there are no significant differences between E-SBUS and E-SBM. Similar results regarding the TFC value expressed in mg RE/g were reported by Li, Y. et al (2021) [59], who studied five varieties of sea berry. Regarding the TFC expressed in mg QE/g, similar results were presented by Criste A. et al (2020) [60], who studied four types of Romanian sea buckthorn berries.…”

Isolation and characterization of auronlignan derivatives with hepatoprotective and hypolipidemic activities from the fruits of Hippophae rhamnoides L.

“…33 The enzyme solution was prepared by the reconstitution of porcine pancreatic lipase in Tris-HCl buffer (0.1 M, pH = 8). The test compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were mixed with enzyme buffer and fostered at 37 °C for 15 min, and then p-nitrophenylbutyrate (10 mM) was added after the incubation of compounds 1-20 with pancreatic lipase solution at 37 °C for 15 min. The absorbance was recorded using an ELISA plate reader at λ = 405 nm.…”

“…A preliminary structure-activity relationship of auronlignan derivatives (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) was summarized according to their chemical structures and bioactivities. Compounds 1, 4, 7, 11, 15, and 19 showed moderate hepatoprotective activities which mainly possessed a 7-prenyl or 4′/5′-furan ring.…”

“…The ethyl acetate (EtOAc) layer of H. rhamnoides L. was determined as the hepatoprotective and hypolipidemic part according to their pharmacological screening results. As a result, nine new auronlignan derivatives (1)(2)(3)(4)(5)(6)(7)(8)(9) and eleven known auronlignan derivatives (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were isolated from the fruits of H. rhamnoides L. for the first time (Fig. 1).…”

“…4,5 With the huge demand of consumers for a healthy natural drug, H. rhamno ides L. has attracted more attention because it contains a variety of obvious bioactive compounds. 6 The results of the modern phytochemicals have revealed that H. rhamnoides L. is rich in flavonoids. 7 In particular, flavonolignans are the important active ingredients of this plant.…”