Impact of DNA methyltransferases on the epigenetic regulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor expression in malignant melanoma

Computational fishing of new DNA methyltransferase inhibitors from natural products

“…The relationship between the hypermethylation of promoter of tumor suppressor genes and cancer development has been clearly demonstrated [3,4,19], suggesting DNMTs as promising drug targets for the discovery of new and more potent/selective anticancer drugs [20]. To date, several DNMT inhibitors (DNMTis) of different structural classes have been published; basically categorized as M a n u s c r i p t 4 nucleoside DNMTis and non-nucleoside analogue DNMTis, Fig.…”

“…The progression of DNA methylation involves a cycle of demethylation, de novo methylation, and methylation maintenance, catalyzed by family enzymes known as DNA methyltransferases (DNMTs, EC#: 2.1.1.37) [3][4][5]. These are responsible of transferring a methyl group from S-adenosyl-L-methionine (SAM) to the carbon-5 position of cytosine in DNA.…”

Epigenetic marks responsible for cadmium-induced melanoma cell overgrowth

“…qPCR was performed by ABI Prism 7500 real-time PCR System (Applied Biosystems, Milan, Italy). Primers and probes were previously reported (Venza et al, 2014;Kanao et al, 2004;Venza et al, 2013;Goyal et al, 2008). The mRNA levels of p14 ARF , p16 INK4A , caspase 8, death receptor 4 (DR4), death receptor 5 (DR5), E-cadherin (CDH1), DNMT1, DNMT3a, and DNMT3b were normalized to endogenous b-actin (Applied Biosystems).…”

Inhibition of IRE1 modifies effect of glucose deprivation on the expression of TNF?-related genes in U87 glioma cells

“…The suppression of ANT2 restores susceptibility of breast cancer cells to TRAIL-induced apoptosis by activating JNK and modulating TRAIL receptor expression: up-regulating the expression of TRAIL death receptors 4 and 5 (DR4 and DR5) and down-regulating the TRAILR4 [49]. Interestingly, the silencing of TNFRSF10D is related to melanoma genesis [50,51]. It is possible that TRAILR4/TNFRSF10D as well as TRAILR2/ TNFRSF10B has relation to regulation of endoplasmic reticulum stress-mediated apoptosis.…”