Impact of DMI and SDHI fungicides on disease control and CYP51 mutations in populations of Zymoseptoria tritici from Northern Europe

Wieczorek, T.; Berg, Gunilla; Semaškienė, Roma; Mehl, Andreas; Sierotzki, Helge; Stammler, G.; Justesen, Annemarie Fejer; Jørgensen, Lise Nistrup

doi:10.1007/s10658-015-0737-1

Cited by 27 publications

(26 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are associated with qualitative resistance (a disruptive shift or single‐step resistance), resulting in a bimodal distribution of phenotypes (individuals either sensitive or resistant), with the resistant phenotype rapidly invading the population . By contrast, DMIs have been used intensively for STB control since the late 1970s; the first resistant strains were selected in the 1980s, and field efficacy has since gradually declined, but this decline has differed between molecules and between sites . A continuum of decreased sensitivity phenotypes (triazole‐resistant phenotypes: TriR) has arisen through progressive accumulation of mutations in the cyp51 target gene, with an overexpression of cyp51 due to insertions in its promoter region, with or without enhanced efflux correlated with three possible insertions in the promoter region of the transporter MFS1 .…”

Section: Introductionmentioning

confidence: 99%

“…Several phenotypic or molecular methods for detecting and quantifying fungicide resistance in Z. tritici have been reported and can be used to investigate resistance dynamics. Several studies have described the variation over space and/or time of the mean EC 50 (half‐maximal effective concentration, the dose at which 50% of the maximal growth inhibition is observed) and its derivatives (‘resistance intensity‐based approaches’), whereas other studies have described variations in the prevalence of several mutations or phenotypes (‘resistance frequency‐based approaches’). Resistance intensity‐based approaches characterize resistance phenotypes clearly, but require strain isolation, which is labour‐intensive and time‐consuming.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spatiotemporal dynamics of fungicide resistance in the wheat pathogen Zymoseptoria tritici in France

Garnault

Duplaix

Leroux

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Management of pesticide resistance is a major issue in modern agricultural systems, particularly in the context of the broader challenge of reducing pesticide use. However, such management must be adapted to resistance dynamics, which remains challenging to predict due to its dependence on many biological traits of pests, interactions with the environment and pesticide use. We retrospectively studied the evolution of reported resistances to four modes of action (benzimidazoles, quinone outside inhibitors, sterol demethylation inhibitors and succinate dehydrogenase inhibitors), in French populations of the wheat pathogen Zymoseptoria tritici. RESULTS: We used statistical models to analyse the Performance trial network data set (2004-2017; ∼ 70 locations in France yearly). They highlighted contrasting behaviours between phenotypes, for example: (i) stable spatial distributions and colonization front structures over time, and (ii) different frequency growth rates at the national scale and between regions. CONCLUSION:We provide a quantitative description of the spatiotemporal patterns of resistance evolution for fungicides with several modes of action. Moreover, we highlight some unexpected resistance dynamics in France, with major differences between the north and south. This complex pattern of resistance evolution in French populations is consistent with previous descriptions of dynamics at the European scale. These results should make it easier to anticipate evolution locally and to improve the management of resistance.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatiotemporal dynamics of fungicide resistance in the wheat pathogen Zymoseptoria tritici in France

Garnault

Duplaix

Leroux

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…Disease control is mainly managed by delayed sowing, the use of fungicides and implementation of resistant cultivars (Ponomarenko, Goodwin, & Kema, ). However, due to sexual reproduction of the pathogen and the intensive use of fungicides particularly in Western Europe, many populations of Z. tritici have developed resistance to these fungicides (Cools & Fraaije, ; Torriani, Brunner, McDonald, & Sierotzki, ; Wieczorek et al., ). A more effective approach to prevent the disease is by breeding for resistance.…”

Section: Introductionmentioning

confidence: 99%

Genomewide association study reveals novel quantitative trait loci associated with resistance towards Septoria tritici blotch in North European winter wheat

Vagndorf

Nielsen²,

Edriss³

et al. 2017

Plant Breeding

Self Cite

View full text Add to dashboard Cite

Fungal diseases are a major constraint for wheat production. Effective disease resistance is essential for ensuring a high production quality and yield. One of the most severe fungal diseases of wheat is Septoria tritici blotch (STB), which influences wheat production across the world. In this study, genomewide association mapping was used to identify new chromosomal regions on the wheat genome conferring effective resistance towards STB. A winter wheat population of 164 North European varieties and breeding lines was genotyped with 15K single nucleotide polymorphism (SNP) wheat array. The varieties were evaluated for STB in field trials at three locations in Denmark and across 3 years. The association analysis revealed four quantitative trait loci, on chromosomes 1B, 2A, 5D and 7A, highly associated with STB resistance. By comparing varieties containing several quantitative trait loci (QTL) with varieties containing none of the found QTL, a significant difference was found in the mean disease score. This indicates that an effective resistance can be obtained by pyramiding several QTL.

show abstract

“…They inhibit the 14α‐sterol demethylase used for synthesizing the essential mycosterol called ergosterol that is encoded by the CYP51 gene (Curvers, Pycke, Kyndt, Haesaert, & Gheysen, ; Duan et al, ; Zhan, Stefanato, & McDonald, ). The DMI fungicides‐resistance mechanism is due to the point mutations of the CYP51 gene as seen in many plant pathogens, for example F. graminearum , Zymoseptoria tritici and Parastagonospora nodorum (Duan et al, ; Leroux, Albertini, Gautier, Gredt, & Walker, ; Pereira, Mcdonald, & Brunner, ; Taher et al, ; Talas & Mcdonald, ; Wieczorek et al, ). Benzimidazole fungicides bind to β‐tubulin to inhibit mycelial growth of plant pathogenic fungi, and the resistance mechanism is the point mutation of amino acids encoding the β‐tubulin in Botrytis cinerea and Sclerotinia sclerotiorum (Liu et al, , ; Zhu, Zhou, Li, Zhu, & Ma, ), or amino acids encoding the β 2 ‐tubulin gene in F. graminearum (Liu, Duan, Ge, Chen, & Zhou, ; Liu et al, ).…”

Section: Discussionmentioning

confidence: 99%

Baseline sensitivity of Fusarium graminearum from wheat fields in Henan, China, to metconazole and analysis of cross resistance with carbendazim and phenamacril

Liu

et al. 2019

Journal of Phytopathology

View full text Add to dashboard Cite

The filamentous plant pathogenic fungus Fusarium graminearum is one of the most important pathogens causing Fusarium head blight (FHB) in wheat in the Henan Province of China. Metconazole is among the demethylation inhibitor (DMI) fungicides with a higher inhibitory activity on the mycelial growth of F. graminearum. In 2016 and 2017, 119 single spore isolates of F. graminearum, prior to being exposed to metconazole, were recovered from 52 wheat fields near 11 cities in Henan Province. The inhibitory activity of metconazole on the mycelia of the Henan F. graminearum population was determined, and EC50 values were calculated. The range of EC50 values of the Henan F. graminearum population to metconazole was 0.0103 to 0.0775 μg/ml with an average EC50 value of 0.0293 ± 0.0114 μg/ml. The sensitivity frequency distribution curve presented a single peak in a narrow range. No cross‐resistance was found between the DMI fungicide metconazole and the benzimidazole fungicide carbendazim or the cyanoacrylate fungicide phenamacril. Therefore, these sensitivity data could be used as the baseline of F. graminearum susceptibility to metconazole in the Henan Province and provide the basis for monitoring metconazole resistance in this area.

show abstract

Impact of DMI and SDHI fungicides on disease control and CYP51 mutations in populations of Zymoseptoria tritici from Northern Europe

Cited by 27 publications

References 22 publications

Spatiotemporal dynamics of fungicide resistance in the wheat pathogen Zymoseptoria tritici in France

Spatiotemporal dynamics of fungicide resistance in the wheat pathogen Zymoseptoria tritici in France

Genomewide association study reveals novel quantitative trait loci associated with resistance towards Septoria tritici blotch in North European winter wheat

Baseline sensitivity of Fusarium graminearum from wheat fields in Henan, China, to metconazole and analysis of cross resistance with carbendazim and phenamacril

Contact Info

Product

Resources

About