Impact of disturbed flow and arterial stiffening on mechanotransduction in endothelial cells

Alonso, Andrea; Ebben, Alessandra; Dabagh, Mahsa

doi:10.1007/s10237-023-01743-0

Cited by 1 publication

(3 citation statements)

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“…The geometric model is depicted in Figures 1B-E, and Movie 1. Each cell (either a cancer cell or fibroblasts represented as fibroblasts) within the tumor is represented as a hexagonal shape [64][65][66][67][68][69][70][71]. Each individual cell has a length of 8.1 µm (X-axis), a width of 7.54 µm (Y-axis), and a height of 8.0 µm (Z-axis) [67][68][69][70][71][72][73][74].…”

Section: Methodsmentioning

confidence: 99%

“…Each individual cell has a length of 8.1 µm (X-axis), a width of 7.54 µm (Y-axis), and a height of 8.0 µm (Z-axis) [67][68][69][70][71][72][73][74]. The ECM has a length 100 µm, 100 µm width, and 100 µm depth [1,[60][61][62][63][64][65][66][67]. The cancer cell was either placed on the outside region of the tumor structure to depict cell-ECM interactions as seen in Figures 1B-C all mechanical parameters applied in the model are summarized in Table 1 [72][73][74][75][76][77][78][79][80][81][82][83][84][85][86].…”

Section: Methodsmentioning

confidence: 99%

“…All stress components are computed and applied to calculate the von Mises stresses (σ ! "# ) a stress invariant usually referred as [64][65][66] This article has been accepted for publication in IEEE Journal of Translational Engineering in Health and Medicine. This is the author's version which has not been fully edited and content may change prior to final publication.…”

Section: Methodsmentioning

confidence: 99%

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Modeling Physical Forces Experienced by Cancer and Stromal Cells Within Different Organ-Specific Tumor Tissue

Connaughton,

Dabagh

2024

IEEE J. Transl. Eng. Health Med.

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Mechanical forces exerted on cancer cells by their microenvironment have been reported to drive cells toward invasive phenotypes by altering cells' motility, proliferation, and apoptosis. These mechanical forces include compressive, tensile, hydrostatic, and shear forces. The importance of forces is then hypothesized to be an alteration of cancer cells' and their microenvironment's biophysical properties as the indicator of a tumor's malignancy state. Our objective is to investigate and quantify the correlation between a tumor's malignancy state and forces experienced by the cancer cells and components of the microenvironment. In this study, we have developed a multicomponent, three-dimensional model of tumor tissue consisting of a cancer cell surrounded by fibroblasts and extracellular matrix (ECM). Our results on three different organs including breast, kidney, and pancreas show that: A) the stresses within tumor tissue are impacted by the organ specific ECM's biophysical properties, B) more invasive cancer cells experience higher stresses, C) in pancreas which has a softer ECM (Young modulus of 1.0 kPa) and stiffer cancer cells (Young modulus of 2.4 kPa and 1.7 kPa) than breast and kidney, cancer cells experienced significantly higher stresses, D) cancer cells in contact with ECM experienced higher stresses compared to cells surrounded by fibroblasts but the area of tumor stroma experiencing high stresses has a maximum length of 40 µm when the cancer cell is surrounded by fibroblasts and 12 µm for when the cancer cell is in vicinity of ECM. This study serves as an important first step in understanding of how the stresses experienced by cancer cells, fibroblasts, and ECM are associated with malignancy states of cancer cells in different organs. The quantification of forces exerted on cancer cells by different organ-specific ECM and at different stages of malignancy will help, first to develop theranostic strategies, second to

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