Impact of different solvents on extraction yield, phenolic composition, in vitro antioxidant and antibacterial activities of deseeded Opuntia stricta fruit

Abstract

Opuntia stricta the shrubby plant is naturally occurring in all parts of the world used as popular ornamental and is known to have medicinal properties due to its phytochemical compositions. In this study, the effect of solvents (80% ethanol, 80% methanol and 80% acetone (v/v) in water) on the extraction yield, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity and antibacterial activity of Opuntia stricta fruit were investigated. For the antioxidant activity assay, three different in-vitro methods DPPH, ABTS and FRAP were followed. The One-way ANOVA calculations showed that the extraction solvents influenced greatly and significantly the TPC, TFC and antioxidant capacity. Overall, 80% ethanol showed highest percentage of extraction yield while 80% acetone extract showed the lowest extraction yield but the highest TPC, TFC and antioxidant activity of Opuntia stricta fruit. In addition, Pearson’s correlation analysis proved a strong correlation between TPC, TFC and all antioxidant methods used. The antibacterial tests demonstrated that 80% methanol and 80% acetone extracts exhibited the highest zones of inhibition (11.66 and 11.33 mm respectively, p > 0.05) against Salmonella thyphimurium. Likewise, the 80% acetone extract revealed the best inhibitory effect contra Escherichia coli (p > 0.05) with an inhibition zone of 11 cm. Unfortunately, it was observed that gram-positive bacteria were less sensitive to all Opuntia stricta extracts compared to the gram-negative bacteria.

Chemical Characterization and Antifungal Activity of Blue Tansy (Tanacetum annuum) Essential Oil and Crude Extracts against Fusarium oxysporum f. sp. albedinis, an Agent Causing Bayoud Disease of Date Palm

“…Moreover, the results demonstrated that bioactive compound contents were affected by solvent polarity and varied from one part to another. This verified what is described in the literature, the solvent with higher polarity extracted more bioactive compounds that are responsible for several bioactivities [14][15][16].…”

Tea (Camellia sinensis) cultivated in three agro-ecological regions of Bangladesh: Unveiling the variability of methylxanthine, bioactive phenolic compound, and antioxidant activity

“…The yield of methanol and hot water extract from different tea leaf were 19.60 ± 5.6 % and 20.07 ± 4.01% respectively, ( Supplementary Table S3 ). El Mannoubi states that the polarity and content of the plant material components are important considerations for selecting an adequate extraction solvent [ 45 ].…”

Melinjo Seeds (Gnetum gnemon L.) Antioxidant Activity and Cytotoxic Effects on MCF-7 Breast Cancer Cells: A Study Based on Tracing of Resveratrol Compound

“…Interestingly, other studies actually show the opposite by showing that antioxidant activity values vary significantly according to the type of solvent and do not depend on the degree of polarity of the organic solvent. [ 21 22 23 ] It is known that various extraction factors, including method, temperature, time, and solvent system, significantly influence the antioxidant quality of plant-derived products. [ 21 ] It can be said that the two extraction stages, namely, the initial extraction stage (crude ethanol extract) and fractionation, are thought to influence the characteristics of the compounds (total phenolics and total flavonoids) on the antioxidant potential of melinjo seeds.…”