Impact of different nitrogen sources on the growth of Arthrospira sp. PCC 8005 under batch and continuous cultivation – A biochemical, transcriptomic and proteomic profile

Deschoenmaeker, Frédéric; Bayon-Vicente, Guillaume; Sachdeva, Neha; Depraetere, Orily; Cabrera, Juan Carlos; Leroy, Baptiste; Muylaert, Koenraad; Wattiez, Ruddy

doi:10.1016/j.biortech.2017.03.145

Cited by 32 publications

(31 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many urease-endowed cyanobacteria can grow on urea as the sole nitrogen source (Collier et al, 1999) but a high-concentration of urea and/or prolonged cultivation on urea (≥10 mM) can be toxic to cyanobacteria. This finding was shown with Arthrospira PCC 8005 (edible cyanobacterium, Deschoenmaeker et al, 2017), Microcystis aeruginosa (fresh water cyanobacterium, Wu et al, 2015), Synechococcus PCC 7002 (costal cyanobacterium, Sakamoto et al, 1998) and Synechocystis PCC 6803 (euryhaline cyanobacterium, Veaudor et al, 2018). The cell death and color change (from blue-green to yellowish) triggered by the prolonged growth on urea could be due to lipid peroxidation, a phenomenon that increases in parallel with cell death and pigment oxidation (Sakamoto et al, 1998).…”

Section: Resultsmentioning

confidence: 61%

Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications

2019

View full text Add to dashboard Cite

Cyanobacteria are widely-diverse prokaryotes that colonize our planet. They use solar energy to assimilate huge amounts of atmospheric CO2 and produce a large part of the biomass and oxygen that sustain most life forms. Cyanobacteria are therefore increasingly studied for basic research objectives, as well as for the photosynthetic production of chemicals with industrial interests. One potential approach to reduce the cost of future bioproduction processes is to couple them with wastewater treatment, often polluted with urea, which in any case is cheaper than nitrate. As of yet, however, research has mostly focused on a very small number of model cyanobacteria growing on nitrate. Thus, the genetic inventory of the cyanobacterial phylum is still insufficiently employed to meaningfully select the right host for the right purpose. This review reports what is known about urea transport and catabolism in cyanobacteria, and what can be inferred from the comparative analysis of the publicly available genome sequence of the 308 cyanobacteria. We found that most cyanobacteria mostly harbor the genes encoding the urea catabolytic enzymes urease (ureABCDEFG), but not systematically, together with the urea transport (urtABCDE). These findings are consistent with the capacity of the few tested cyanobacteria that grow on urea as the sole nitrogen source. They also indicate that urease is important for the detoxification of internally generated urea (re-cycling its carbon and nitrogen). In contrast, several cyanobacteria have urtABCDE but not ureABCDEFG, suggesting that urtABCDE could operate in the transport of not only urea but also of other nutrients. Only four cyanobacteria appeared to have the genes encoding the urea carboxylase (uc) and allophanate hydrolase (ah) enzymes that sequentially catabolize urea. Three of these cyanobacteria belongs to the genera Gloeobacter and Gloeomargarita that have likely diverged early from other cyanobacteria, suggesting that the urea carboxylase and allophanate hydrolase enzymes appeared in cyanobacteria before urease.

show abstract

Section: Resultsmentioning

confidence: 61%

Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications

2019

View full text Add to dashboard Cite

show abstract

“…M. bovis BCG was treated with 0.4 g/ml INH (with or without 10 nM Q203) for 7 h and harvested for proteomic analysis. Protein extraction was described previously (63), and the detailed proteomic procedure can be found in the supplemental material. Protein hits with a P value of Ͻ0.05 and a fold change of Ͻ0.8 or Ͼ1.2 were further analyzed.…”

Section: Methodsmentioning

confidence: 99%

Isoniazid Bactericidal Activity Involves Electron Transport Chain Perturbation

Zeng

Soetaert

Ravon

et al. 2019

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

Accumulating evidence suggests that the bactericidal activity of some antibiotics may not be directly initiated by target inhibition. The activity of isoniazid (INH), a key first-line bactericidal antituberculosis drug currently known to inhibit mycolic acid synthesis, becomes extremely poor under stress conditions, such as hypoxia and starvation. This suggests that the target inhibition may not fully explain the bactericidal activity of the drug. Here, we report that INH rapidly increased Mycobacterium bovis BCG cellular ATP levels and enhanced oxygen consumption. The INH-triggered ATP increase and bactericidal activity were strongly compromised by Q203 and bedaquiline, which inhibit mycobacterial cytochrome bc1 and FoF1 ATP synthase, respectively. Moreover, the antioxidant N-acetylcysteine (NAC) but not 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) abrogated the INH-triggered ATP increase and killing. These results reveal a link between the energetic (ATP) perturbation and INH’s killing. Furthermore, the INH-induced energetic perturbation and killing were also abrogated by chemical inhibition of NADH dehydrogenases (NDHs) and succinate dehydrogenases (SDHs), linking INH’s bactericidal activity further to the electron transport chain (ETC) perturbation. This notion was also supported by the observation that INH dissipated mycobacterial membrane potential. Importantly, inhibition of cytochrome bd oxidase significantly reduced cell recovery during INH challenge in a culture settling model, suggesting that the respiratory reprogramming to the cytochrome bd oxidase contributes to the escape of INH killing. This study implicates mycobacterial ETC perturbation through NDHs, SDHs, cytochrome bc1, and FoF1 ATP synthase in INH’s bactericidal activity and pinpoints the participation of the cytochrome bd oxidase in protection against this drug under stress conditions.

show abstract

“…Alternatively, WT BCG was grown as biofilm cultures for 25 days in 0.2, 2, 4, 6, and 8% glycerol Sauton’s medium for proteomic analysis. Protein extraction was described previously (Deschoenmaeker et al, 2017). Detailed proteomic procedure can be found either in the Supplementary Material or in our previous publication (Zeng et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Cpn60.1 (GroEL1) Contributes to Mycobacterial Crabtree Effect: Implications for Biofilm Formation

et al. 2019

Self Cite

View full text Add to dashboard Cite

Biofilm formation is a survival strategy for microorganisms facing a hostile environment. Under biofilm, bacteria are better protected against antibacterial drugs and the immune response, increasing treatment difficulty, as persistent populations recalcitrant to chemotherapy are promoted. Deciphering mechanisms leading to biofilms could, thus, be beneficial to obtain new antibacterial drug candidates. Here, we show that mycobacterial biofilm formation is linked to excess glycerol adaptation and the concomitant establishment of the Crabtree effect. This effect is characterized by respiratory reprogramming, ATP downregulation, and secretion of various metabolites including pyruvate, acetate, succinate, and glutamate. Interestingly, the Crabtree effect was abnormal in a mycobacterial strain deficient for Cpn60.1 (GroEL1). Indeed, this mutant strain had a compromised ability to downregulate ATP and secreted more pyruvate, acetate, succinate, and glutamate in the culture medium. Importantly, the mutant strain had higher intracellular pyruvate and produced more toxic methylglyoxal, suggesting a glycolytic stress leading to growth stasis and consequently biofilm failure. This study demonstrates, for the first time, the link between mycobacterial biofilm formation and the Crabtree effect.

show abstract

Impact of different nitrogen sources on the growth of Arthrospira sp. PCC 8005 under batch and continuous cultivation – A biochemical, transcriptomic and proteomic profile

Cited by 32 publications

References 49 publications

Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications

Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications

Isoniazid Bactericidal Activity Involves Electron Transport Chain Perturbation

Cpn60.1 (GroEL1) Contributes to Mycobacterial Crabtree Effect: Implications for Biofilm Formation

Contact Info

Product

Resources

About