Impact of dietary Mannan-oligosaccharide and β-Glucan supplementation on growth, histopathology, E-coli colonization and hepatic transcripts of TNF-α and NF- ϰB of broiler challenged with E. coli O78

Abstract

Background: Using probiotics have become popular. They are considered an alternative to Antibiotic Growth Promoters (AGP). Probiotics are supplemented into animal feed for improving growth performance along with preventing and controlling enteric pathogens. The aim of this work was to study the impact of dietary supplementation of Mannan-oligosaccharide and β-Glucan (Agrimos®) on broiler challenged with Escherichia coli O78 (E. coli O78 - marked with an antibiotic (320 μg ciprofloxacin/ml broth) on growth performance, serum biochemistry, immune organs-histopathology, E-coli colonization, and hepatic transcripts of Tumor necrosis factor-alpha (TNF-α) and Nuclear factor-kappa B (NF-ϰB). Methods: A total of 125 one-day-old chicks were used for conducting the experiment. Five chicks were slaughtered for measuring the initial weight of lymphoid tissue; the remaining chicks (120) were allotted into four groups according to Mannan-oligosaccharide and β-Glucan supplementation and E. coli infection. The data were analyzed using SPSS version 16. Results: Results indicated significant alteration of growth performance, serum biochemistry, selected liver gene expression with pathological lesions, especially in lymphoid organs due to E. coli infection, which were mitigated by Mannan-oligosaccharide and β-Glucan supplementation. Conclusion: It could be concluded Mannan-oligosaccharide and β-Glucan supplementation in broiler's diet improved the immune response of broilers and mitigated pathological lesion resulted from E. coli infection.

Evaluation of cefquinome's efficacy in controlling avian colibacillosis and detection of its residues using high performance liquid chromatography (HPLC)

“…In this study, E. coli infection induced an elevation in MDA level in infected group related to excessive production of free radicals causing stress and cellular toxicity, this results agreed with ( Fadl et al, 2020 ) who found high levels of MDA in E. coli infected broilers.…”

“…In this study, decreased body weight, weight gain and increased feed conversion rate throughout the experimental period were clearly appeared in infected broilers, which might be attributed to intestinal damage and poor digestion caused by microorganisms; these results were agreed with ( Mabrouk, 2016 , Fadl et al, 2020 ). Cefquinome treated group showed decreased feed conversion compared with non-treated, this previous data is supported by ( Radwan and Radi, 2010 ).…”

Ameliorative Effect of Nanocurcumin and/or Saccharomyces Cell Wall Against Aflatoxicosis in Broilers

“…This is indicative of the toxic effect of a atoxin B1 on the liver and kidney and is an indicator of diminished protein synthesis [46]. As shown in our study, nanocurcumin and/or yeast cell wall extract that included in broiler diets improved the hepatic function as serum total protein, albumin, and globulin concentrations were increased and this is compatible with previous studies [49,50,51]. On the other hand, Li et al [52] explained that YCW incorporation did not affect the same biochemical indices, including total protein, albumin, and globulin.…”

“…Ma et al [68] reported AFB1 treatment increased the protein levels of NF-KB p65. Moreover, when Fadl et al [51] studied the effect of dietary Mannan-oligosaccharide and β-Glucan (yeast cell wall extract) supplementation on hepatic transcripts of NF-KB of broiler challenged with E. coli O78, they found that the gene expression of NF KB was reduced and this indicates the protective effects and anti-in ammatory properties of YCW extract in broilers. This result in similar to the current result in spite of the current result obtained the expression of the NFkB P65 itself in the liver tissue of AF affected broilers.…”

Interleukin gene expression in broiler chickens infected by different Escherichia coli serotypes

“…This result is consistent with a previous study [ 38 ] that reported mouth breathing, sneezing, ruffled feathers, weights loss, diarrhea, loss of appetite, diarrhea, and mortality (30%) in chickens infected with E. coli O78. Another study indicated depression with whitish diarrhea in broiler chickens infected experimentally with E. coli O78 [ 21 , 39 ]. Birds exhibited a 12.82% mortality after 48 h among birds challenged with E. coli .…”