Impact of dietary fibre:starch ratio in shaping caecal archaea revealed in rabbits

Zhu, Yan; Sun, Yu; Wang, C.; Li, F.

doi:10.1111/jpn.12585

Cited by 14 publications

(15 citation statements)

References 28 publications

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“…Thus, the methanogenic archaea are widely found in the gastrointestinal tract of herbivores and many methanogens have been isolated and identified from different animals (Jin et al, 2017; Wright et al, 2004). However, almost all of the archaea found in the gastrointestinal tract of animals originated from Euryarchaeota (Jin et al, 2017; Zhu et al, 2016). This was consistent with our study.…”

Section: Discussionmentioning

confidence: 99%

Effects of particle size of ground alfalfa hay on caecal bacteria and archaea populations of rabbits

Yuan

Liu

Wang

et al. 2019

PeerJ

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This work was aimed to investigate the effects of the different particle size of ground alfalfa hay on caecal microbial and archeal communities of rabbits. One hundred-twenty New Zealand rabbits (950.3 ± 8.82 g) were allocated into four treatments, with five replicates in each treatment and six rabbits in each replicate. The particle sizes of the alfalfa meal in the four treatment diets were 2,500, 1,000, 100 and 10 µm respectively, while the other ingredients were ground through a 2.5 mm sieve. High-throughput sequencing technology was applied to examine the differences in bacteria and methanogenic archaea diversity in the caecum of the four treatment groups of rabbits. A total of 745,946 bacterial sequences (a mean of 31,081 ± 13,901 sequences per sample) and 539,227 archaeal sequences (a mean of 22,468 ± 2,443 sequences per sample) were recovered from twenty-four caecal samples, and were clustered into 9,953 and 2,246 OTUs respectively. A total of 26 bacterial phyla with 465 genera and three archaeal phyla with 10 genera were identified after taxonomic summarization. Bioinformatic analyses illustrated that Firmicutes (58.69% ∼ 68.50%) and Bacteroidetes (23.96% ∼ 36.05%) were the two most predominant bacterial phyla and Euryarchaeota (over 99.9%) was the most predominant archaeal phyla in the caecum of all rabbits. At genus level, as the particle size of alfalfa decreased from 2,500 to 10 µm, the relative abundances of Ruminococcaceae UCG-014 (P < 0.001) and Lactobacillus (P = 0.043) were increased and Ruminococcaceae UCG-005 (P = 0.012) was increased first and then decreased when the alfalfa particle size decreased, while Lachnospiraceae NK4A136 group (P = 0.016), Ruminococcaceae NK4A214 (P = 0.044), Christensenellaceae R-7 group (P = 0.019), Lachnospiraceae other (Family) (P = 0.011) and Ruminococcaceae UCG-013 (P = 0.021) were decreased. The relative abundance of Methanobrevibacter was increased from 62.48% to 90.40% (P < 0.001), whereas the relative abundance of Methanosphaera was reduced from 35.47% to 8.62% (P < 0.001). In conclusion, as the particle size of alfalfa meal decreased, both the bacterial and archaeal population in the caecum of rabbit experienced alterations, however archaea response earlier than bacteria to the decrease of alfalfa meal particle size.

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Section: Discussionmentioning

confidence: 99%

Effects of particle size of ground alfalfa hay on caecal bacteria and archaea populations of rabbits

Yuan

Liu

Wang

et al. 2019

PeerJ

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“…Venn diagrams, PCA, and heat map plots of the bacterial communities derived from rabbit cecum showed that a shift of dietary fiber can lead to quick changes in the composition of the microbiota, which is a reflection of a rapid adaptation to reach a new equilibrium in response to a nutritional disturbance (Michelland et al., ; Zhu, Sun, Wang, & Li, ). Meanwhile, we found that in the cecum, the structure of the bacterial community has no difference between rabbits of the same age or fed the same diet treatment (Michelland et al., ).…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary fiber levels on bacterial composition with age in the cecum of meat rabbits

Zhou

et al. 2018

MicrobiologyOpen

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This study investigated the influence of dietary fiber levels on the growth performance, digestion, metabolism, and cecal microbial community of rabbits with different diets at different age. The different levels of dietary natural detergent fiber (NDF) were formulated accordingly: 400(A), 350(B), 300(C), 250(D) g/kg original matter basis, respectively; the different ages were 52, 62, and 72 days. With NDF increasing, the average daily feed intake (ADFI) and feed conversion rate (FCR) were increased, whereas average daily gain (ADG) and mortality were decreased (p < 0.05). The stomach relative weight, stomach content relative weight, cecal relative weight, and cecal content weight increased with increasing NDF (p < 0.05). The NH -N concentration of cecum dropped when the dietary NDF increased (p < 0.05). The diversity of the total microbiota increased significantly in Diets B, C (p = 0.011), and reached the lowest in 52 days for all diet groups. The richness index was decreased significantly in Diet A, D (p < 0.05) and in 62 days (p < 0.001), respectively. The phylum Firmicutes was higher (p < 0.01) in rabbits fed Diets B, C than Diets A, D and Bacteroidetes was highest in Diets C, D, and Proteobacteria was the highest in Diet A (p < 0.001). Among the classified genera, there were 14 that had levels of abundance of more than 1% and were commonly shared by all samples. Ruminococcus spp. that produced volatile fatty acid (VFA) abundance was highest from Diets B, C at 52 and 62 days. It is interesting to note that Bifidobacterium from Diet C was the most abundant genus during the entire experimental period (p < 0.01). The data from Venn diagrams, principal component analysis (PCA), and heat map plots of the bacterial communities showed that there were more groups of shared microbiota with aging. The above results indicate the cecal microbiota controlled by the 350 g/kg NDF diet can prevent gastrointestinal distress and exhibit good production performance.

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“…In the current study, the diversity of methanogens decreased with the decrease in alfalfa meal particle size and Witzig et al [ 53 ] reported that ruminant microbial community structure changed according to feed particle size. However, Zhu et al [ 17 ] reported no significant differences in the diversity and abundance of caecal archaeal community of rabbits that were fed diets with different dietary fibre-to-starch ratios.…”

Section: Discussionmentioning

confidence: 99%

“…have been highlighted [ 14 – 15 ]. Although many studies involve cecal fermentation, the study of methane production by rabbit is limited [ 16 – 17 ]. Several previous investigations mainly conducted were in vitro experiments [ 18 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of the particle of ground alfalfa hay on the growth performance, methane production and archaeal populations of rabbits

et al. 2018

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The world's annual output of rabbits is over 1.2 billion, therefore this sector is also one of the sources of greenhouse gases in livestock production. One hundred-twenty New Zealand rabbits were allocated into four treatments, five replicates in each treatment and six rabbits in each replicate to examine the effect of grinding alfalfa hay to different sizes on growth performance, methane production and cecal archaeal populations. The particle sizes of the alfalfa meal in the four treatment diets were 2500, 1000, 100 and 10 μm, while the other ingredients were ground through a 2.5 mm sieve. The average daily gain (ADG) and average daily feed intake (ADFI) increased (P<0.001) as the particle size decreased, but the feed conversion ratio (FCR) was not affected (P = 0.305). The digestibility of neutral detergent fiber (NDF) (P = 0.006) and acid detergent fiber (ADF) (P<0.006) increased while the greatest digestibility of crude protein (CP) was obtained in 1000 um group (P = 0.015). The rabbits produced more methane (CH4, L/kgBM0.75/d) with decreasing alfalfa particle size (P<0.001). The molar proportion of acetic acid and propionic acid decreased (P<0.001) at the cost of butyric acid (P<0.001). The greatest villus height:crypt depth ratio were obtained in 1000 μm group, and the decrease in the alfalfa hay particle size decreased the jejunum and ilem villus height:crypt depth ratio (P<0.05). The gastric muscular and mucosal thickness decreased with decreasing alfalfa particle size (P<0.05). Archaea diversity decreased with decreasing alfalfa particle size, and the relative abundance of genus Methanobrevibacter increased (P<0.001) while the genus Methanosphaera decreased (P<0.001). It is concluded that a finer particle size favors the growth of genus Methanobrevibacter, which produces more methane but promotes the growth performance of rabbits.

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Impact of dietary fibre:starch ratio in shaping caecal archaea revealed in rabbits

Cited by 14 publications

References 28 publications

Effects of particle size of ground alfalfa hay on caecal bacteria and archaea populations of rabbits

Effects of particle size of ground alfalfa hay on caecal bacteria and archaea populations of rabbits

Effect of dietary fiber levels on bacterial composition with age in the cecum of meat rabbits

Effects of the particle of ground alfalfa hay on the growth performance, methane production and archaeal populations of rabbits

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