Impact of diesel particulate matter on the olfactory bulb of mice: insights from behavioral, histological, and molecular assessments

Lee, Jeongmin; Weerasinghe-Mudiyanselage, Poornima D. E.; Kim, Bohye; Kang, Sohi; Kim, Joong-Sun; Moon, Changjong

doi:10.1007/s13273-023-00414-6

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…Immunohistochemical analyses showed a significant reduction in TH-positive immunoreactive cells and fibers in both the ipsilateral substantia nigra ( Figure 1 (B); t(4) = 8.752; P < 0.001) and striatum ( Figure 1 (C); t(4) = 8.738; P < 0.001) after unilateral 6-OHDA treatment ( Figure 1 (B)), consistent with a prior study (Kim et al 2022 ; Lee et al 2023 ).…”

Section: Resultssupporting

confidence: 90%

Identifying candidate genes associated with hippocampal dysfunction in a hemiparkinsonian rat model by transcriptomic profiling

Kim,

Hong,

Lee

et al. 2024

Animal Cells and Systems

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Parkinson’s disease (PD) often results in hippocampal dysfunction, which leads to cognitive and emotional challenges and synaptic irregularities. This study attempted to assess behavioral anomalies and identify differentially expressed genes (DEGs) within the hippocampus of a hemiparkinsonian rat model to potentially uncover novel genetic candidates linked to hippocampal dysfunction. Striatal 6-hydroxydopamine (6-OHDA) infusions were performed unilaterally in the brains of adult SD rats, while dopaminergic impairments were verified in rats with 6-OHDA-lesioned striata. RNA sequencing and gene expression analysis unveiled 1018 DEGs in the ipsilateral rat hippocampus following 6-OHDA infusion: 631 genes exhibited upregulation, while 387 genes were downregulated (with FDR-adjusted p -value < 0.05 and absolute fold-change > 1.5). Gene ontology analysis of DEGs indicated that alterations in the hippocampi of 6-OHDA-lesioned rats were primarily associated with synaptic signaling, axon development, behavior, postsynaptic membrane, synaptic membrane, neurotransmitter receptor activity, and peptide receptor activity. The Kyoto Encyclopedia of Genes and Genomes analysis of DEGs demonstrated significant enrichment of the neuroactive ligand–receptor interaction, calcium signaling pathway, cAMP signaling pathway, axon guidance, and notch signaling pathway in rat hippocampi that had been subjected to striatal 6-OHDA infusion. STRING analysis confirmed a notable upregulation of eight hub genes ( Notch3 , Gng4 , Itga3 , Grin2d , Hgf , Fgf11 , Htr3a , and Col6a2 ), along with a significant downregulation of two hub genes ( Itga11 and Plp1 ), as validated by reverse transcription-quantitative polymerase chain reaction. This study provides a comprehensive transcriptomic profile of the hippocampi in a hemiparkinsonian rat model, thereby offering insights into the signaling pathways underlying hippocampal dysfunction.

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Section: Resultssupporting

confidence: 90%

Identifying candidate genes associated with hippocampal dysfunction in a hemiparkinsonian rat model by transcriptomic profiling

Kim,

Hong,

Lee

et al. 2024

Animal Cells and Systems

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“…The impact of exposure to environmental pollutants in early life on neurodevelopment and the nervous system is an area of growing interest [ 12 ]. Therefore, the effects of environmentally hazardous substances on the human body are being explored using various methods [ 13 , 14 , 15 ]. However, elucidating the relationship between environmental exposure and human health can be challenging.…”

Section: Introductionmentioning

confidence: 99%

Potential Effects of Low-Level Toluene Exposure on the Nervous System of Mothers and Infants

Yu,

Kim,

Choo

et al. 2024

IJMS

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In day-to-day living, individuals are exposed to various environmentally hazardous substances that have been associated with diverse diseases. Exposure to air pollutants can occur during breathing, posing a considerable risk to those with environmental health vulnerabilities. Among vulnerable individuals, maternal exposure can negatively impact the mother and child in utero. The developing fetus is particularly vulnerable to environmentally hazardous substances, with potentially greater implications. Among air pollutants, toluene is neurotoxic, and its effects have been widely explored. However, the impact of low-level toluene exposure in daily life remains unclear. Herein, we evaluated 194 mothers and infants from the Growing children’s health and Evaluation of Environment (GREEN) cohort to determine the possible effects of early-life toluene exposure on the nervous system. Using Omics experiments, the effects of toluene were confirmed based on epigenetic changes and altered mRNA expression. Various epigenetic changes were identified, with upregulated expression potentially contributing to diseases such as glioblastoma and Alzheimer’s, and downregulated expression being associated with structural neuronal abnormalities. These findings were detected in both maternal and infant groups, suggesting that maternal exposure to environmental hazardous substances can negatively impact the fetus. Our findings will facilitate the establishment of environmental health policies, including the management of environmentally hazardous substances for vulnerable groups.

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The Chronic Toxicity of Endocrine-Disrupting Chemical to Daphnia magna: A Transcriptome and Network Analysis of TNT Exposure

Lee,

Kim,

Shin

et al. 2024

IJMS

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Endocrine-disrupting chemicals (EDCs) impair growth and development. While EDCs can occur naturally in aquatic ecosystems, they are continuously introduced through anthropogenic activities such as industrial effluents, pharmaceutical production, wastewater, and mining. To elucidate the chronic toxicological effects of endocrine-disrupting chemicals (EDCs) on aquatic organisms, we collected experimental data from a standardized chronic exposure test using Daphnia magna (D. magna), individuals of which were exposed to a potential EDC, trinitrotoluene (TNT). The chronic toxicity effects of this compound were explored through differential gene expression, gene ontology, network construction, and putative adverse outcome pathway (AOP) proposition. Our findings suggest that TNT has detrimental effects on the upstream signaling of Tcf/Lef, potentially adversely impacting oocyte maturation and early development. This study employs diverse bioinformatics approaches to elucidate the gene-level toxicological effects of chronic TNT exposure on aquatic ecosystems. The results provide valuable insights into the molecular mechanisms of the adverse impacts of TNT through network construction and putative AOP proposition.

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Impact of diesel particulate matter on the olfactory bulb of mice: insights from behavioral, histological, and molecular assessments

Cited by 3 publications

References 46 publications

Identifying candidate genes associated with hippocampal dysfunction in a hemiparkinsonian rat model by transcriptomic profiling

Identifying candidate genes associated with hippocampal dysfunction in a hemiparkinsonian rat model by transcriptomic profiling

Potential Effects of Low-Level Toluene Exposure on the Nervous System of Mothers and Infants

The Chronic Toxicity of Endocrine-Disrupting Chemical to Daphnia magna: A Transcriptome and Network Analysis of TNT Exposure

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