Impact of Detergents on Membrane Protein Complex Isolation

Lee, Yu Chen; Bååth, Jenny Arnling; Bastle, Ryan M.; Bhattacharjee, Sonali; Cantoria, Mary Jo; Dornan, Mark H.; Gamero-Estevez, Enrique; Ford, Lenzie; Hálová, Lenka; Kernan, Jennifer C.; Kürten, Charlotte; Li, Siran; Martinez, Jerahme; Sachan, Nalani; Sarr, Médoune; Shan, Xiwei; Subramanian, Nandhitha; Rivera, Keith; Pappin, Darryl; Lin, Sue Hwa

doi:10.1021/acs.jproteome.7b00599

Cited by 26 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, they have a wide range of applications and development in food, cosmetic, pharmaceutical industries, and scientific research (de Foresta et al, ; Jastrzebska et al, ; Ni et al, ; Pillion et al, ; Raman et al, ; Rifkin et al, ; Santonicola et al, ; Weber and Benning, ; Zhang et al, ). They have been used in drug delivery (Ahmad et al, ), thermal stabilization (Ahmad et al, ), hydrolytic metabolism (Weber and Benning, ), salt tolerance (Zhang et al, ), formation of higher‐ordered structure (Jastrzebska et al, ), co‐immunoprecipitation (Lee et al, ), and structure analysis (Ni et al, ), as well as therapeutic adjuvants (Rifkin et al, ), permeation enhancers (Pillion et al, ), absorption enhancers (Gradauer et al, ), and membrane protein stabilizers against denaturation (Das et al, ; de Foresta et al, ; Jastrzebska et al, ; Klammt et al, ; Newby et al, ; Raman et al, ; Santonicola et al, ; Whorton and MacKinnon, ).…”

Section: Introductionmentioning

confidence: 99%

“…Weber and Benning, 1984;Zhang et al, 1996). They have been used in drug delivery (Ahmad et al, 2014), thermal stabilization (Ahmad et al, 2014), hydrolytic metabolism (Weber and Benning, 1984), salt tolerance (Zhang et al, 1996), formation of higher-ordered structure (Jastrzebska et al, 2006), co-immunoprecipitation (Lee et al, 2018), and structure analysis (Ni et al, 2011), as well as therapeutic adjuvants (Rifkin et al, 2011), permeation enhancers (Pillion et al, 2002), absorption enhancers (Gradauer et al, 2017), and membrane protein stabilizers against denaturation (Das et al, 2017;de Foresta et al, 2011;Jastrzebska et al, 2006;Klammt et al, 2012;Newby et al, 2009;Raman et al, 2006;Santonicola et al, 2008;Whorton and MacKinnon, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Solution Properties of Alkyl β‐D‐Maltosides

Zhencao

Chen

et al. 2019

J Surfact & Detergents

View full text Add to dashboard Cite

Alkyl β‐D‐maltosides are an important class of sugar‐based nonionic surfactants and have been widely studied. Nevertheless, it is still necessary to investigate further their amphiphilic structure‐surface property relationships. In this article, we reported a series of properties of synthetic alkyl β‐D‐maltosides (6a–6i, n = 6–18) including their hydrophilic–lipophilic balance (HLB) number, water solubility, hygroscopicity, moisture‐retention capacity, foaming ability, surface tension, thermotropic phase behavior, and skin irritation. Their HLB number and water solubility decreased with increasing alkyl chain length. Hexyl β‐D‐maltoside exhibited the strongest hygroscopicity and moisture‐retention capacity. Decyl β‐D‐maltoside and dodecyl β‐D‐maltoside possessed excellent foaming power and foaming stability. Furthermore, the critical micelle concentration (CMC) of alkyl β‐D‐maltoside (6a–6g, n = 6–14) and their surface tension at CMC decreased with increasing alkyl chain length. At last, alkyl β‐D‐maltosides (6a–6g) should be considered as safe surfactants by the skin irritation assessment.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Solution Properties of Alkyl β‐D‐Maltosides

Zhencao

Chen

et al. 2019

J Surfact & Detergents

View full text Add to dashboard Cite

show abstract

“…Cre dependent Cas9 knock in mouse was generated and using these mice, KRAS, p53 and LKB1 gene mutations were modeled for lung adenocarcinoma [109]. An intraductal injection of lentivirus-encoding Cas9 was used to generate invasive lobular breast carcinoma (ILC) with conditional alleles of Cdh1 gene, encoding E-cadherin [110, 111].…”

Section: Therapeutic Targeting Of Crispr In Cancer and Animal Modelsmentioning

confidence: 99%

CRISPR–Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics

et al. 2019

Self Cite

View full text Add to dashboard Cite

Genome editing allows for the precise manipulation of DNA sequences in a cell making this technology essential for understanding gene function. CRISPR/Cas9 is a targeted genome-editing platform derived from bacterial adaptive immune system and has been repurposed into a genome-editing tool. The RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, making this technology easier, more efficient, scalable and an indispensable tool in biological research. This technology has helped genetically engineer animal models to understand disease mechanisms and elucidate molecular details that can be exploited for improved therapeutic outcomes. In this review, we describe the CRISPR–Cas9 gene-editing mechanism, CRISPR-screening methods, therapeutic targeting of CRISPR in animal models and in cancer immunotherapy. We also discuss the ongoing clinical trials using this tool, limitations of this tool that might impede the clinical applicability of CRISPR–Cas9 and future directions for developing effective CRISPR–Cas9 delivery systems that may improve cancer therapeutics.

show abstract

“…Due to their association with the cell membrane, phage receptors, especially membrane proteins, can be challenging to study. Proteinaceous phage receptors are generally incorporated into the bacterial cell membrane and are thereby dependent on this membrane to maintain correct folding (19), which makes them notoriously difficult to isolate (20,21). This limits the number of phages of which the interaction with a receptor can be studied in detail.…”

Section: Introductionmentioning

confidence: 99%

Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions

Jonge

Sibinga

Boright

et al. 2020

J Virol

View full text Add to dashboard Cite

The infection of a bacterium by a phage starts with attachment to a receptor molecule on the host cell surface by the phage. As receptor-phage interactions are crucial to successful infections, they are major determinants of phage host-range and by extension of the broader effects that phages have on bacterial communities. Many receptor molecules, particularly membrane proteins, are difficult to isolate because their stability is supported by their native membrane environments. Styrene maleic acid lipid particles (SMALPs), a recent advance in membrane protein studies, are the result of membrane solubilizations by styrene maleic acid (SMA) co-polymer chains. SMALPs thereby allow for isolation of membrane proteins while maintaining their native environment. Here, we explore SMALPs as a tool to isolate and study phage-receptor interactions. We show that SMALPs produced from taxonomically distant bacterial membranes allow for receptor-specific decrease of viable phage counts of several model phages that span the three largest phage families. After characterizing the effects of incubation time and SMALP concentration on the activity of three distinct phages, we present evidence that the interaction between two model phages and SMALPs is specific to bacterial species and the phage receptor molecule. These interactions additionally lead to DNA ejection by nearly all particles at high phage titers. We conclude that SMALPs are a potentially highly useful tool for phage host-interaction studies. IMPORTANCE Bacteriophages (viruses that infect bacteria or phages) impact every microbial community. All phage infections start with the binding of the viral particle to a specific receptor molecule on the host cell surface. Due to its importance in phage infections, this first step is of interest to many phage-related research and applications. However, many phage receptors are difficult to isolate. Styrene maleic acid lipid particles (SMALPs) are a recently developed approach to isolate membrane proteins in their native environment. In this study we explore SMALPs as a tool to study phage-receptor interactions. We find that different phage species bind to SMALPs, while maintaining specificity to their receptor. We then characterize the time and concentration dependence of phage-SMALP interactions, and furthermore show that they lead to genome ejection by the phage. Combined, the results presented here show that SMALPs are a useful tool for future studies of phage-receptor interactions.

show abstract

Impact of Detergents on Membrane Protein Complex Isolation

Cited by 26 publications

References 19 publications

Solution Properties of Alkyl β‐D‐Maltosides

Solution Properties of Alkyl β‐D‐Maltosides

CRISPR–Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics

Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions

Contact Info

Product

Resources

About