Impact of deep sequencing on hepatocellular carcinoma utilizing high-throughput technology

Hashem, Esraa M.; Mabrouk, Mai S.; Eldeib, Ayman M.

doi:10.1007/s13721-020-00242-x

Cited by 1 publication

(1 citation statement)

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“…Consequently, it is urgently needed to continue looking for novel biomarkers. Recently, NGS technologies have shown to be a potent new force in the arsenal of cancer geneticists, who support the investigation of cancer genomes at higher resolutions ( 18 ). The patterns of miRNAs in diverse malignancies and normal tissues vary, and they are crucial in the beginning and progression of cancer ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

miRNome of Child A hepatocellular carcinoma in Egyptian patients

et al. 2023

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IntroductionHepatocellular carcinoma (HCC) has different etiologies that contribute to its heterogeneity. In regards to the number of HCC patients, Egypt ranks third in Africa and fifteenth worldwide. Despite significant advancements in HCC diagnosis and treatment, the precise biology of the tumor is still not fully understood, which has a negative impact on patient outcomes.MethodsAdvances in next-generation sequencing (NGS) have increased our knowledge of the molecular complexity of HCC.Results & discussionIn this research, 16 HCC and 6 tumor adjacent tissues (control) of Child A Egyptian patients were successfully profiled for the expression profile of miRNAs by NGS. Forty-one differentially expressed miRNAs (DEMs) were found by differential expression analysis, with 31 being upregulated and 10 being downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was then conducted on these differentially expressed miRNAs revealing that Sensitivity and specificity analysis showed that hsa-miR-4488, hsa-miR-3178, and hsa-miR-3182 were unique miRNAs as they are expressed in HCC tissues only. These miRNAs were all highly involved in AMPK signaling pathways. However, hsa-miR-214-3p was expressed in control tissues about eight times higher than in cancer tissues and was most abundant in “pathways in cancer and PI3K-Akt signaling pathway” KEGG terms. As promising HCC diagnostic markers, we here suggest hsa-miR-4488, hsa-miR-3178, hsa-miR-3182, and hsa-miR-214-3p. We further urge future research to confirm these markers' diagnostic and prognostic potential as well as their roles in the pathophysiology of HCC.

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Section: Introductionmentioning

confidence: 99%