Impact of Curcumin Supersaturation in Antibacterial Photodynamic Therapy—Effect of Cyclodextrin Type and Amount: Studies on Curcumin and Curcuminoides XLV

Hegge, Anne Bee; Nielsen, Thorbjørn Terndrup; Larsen, Kim Lambertsen; Bruzell, Ellen; Tønnesen, Hanne

doi:10.1002/jps.23046

Cited by 60 publications

(52 citation statements)

References 27 publications

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“…In addition, nanoparticles reduce PS aggregation, which is known to decrease photodynamic reactivity, and can promote selectivity by passive or active targeting. In this study, encapsulation in a hydrophilic matrix was used to overcome the limitations observed with other formulations [18,20,21,46] and allows for sustained release, important for topical drug delivery.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, other PSs with different properties and structures, such as curcumin, have not been utilized. Curcumin (diferuloylmethane) is a yellow crystalline compound isolated from the spice turmeric that has emerged as a potential photosensitizing compound [16][17][18][19][20][21][22][23][24][25][26]. Curcumin absorbs in the 408-434 nm range [17], and requires blue light for photoactivation.…”

Section: Introductionmentioning

confidence: 99%

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Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by the induction of nitrosative stress after photodynamic activation

2015

Journal of the American Academy of Dermatology

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Antimicrobial photodynamic inhibition (aPI) utilizes radical stress generated from the excitation of a photosensitizer (PS) with light to destroy pathogens. Its use against Trichophyton rubrum, a dermatophytic fungus with increasing incidence and resistance, has not been well characterized. Our aim was to evaluate the mechanism of action of aPI against T. rubrum using curcumin as the PS in both free and nanoparticle (curc-np) form. Nanocarriers stabilize curcumin and allow for enhanced solubility and PS delivery. Curcumin aPI, at optimal conditions of 10 μg/mL of PS with 10 J/cm 2 of blue light (417 ± 5 nm), completely inhibited fungal growth (p<0.0001) via induction of reactive oxygen (ROS) and nitrogen species (RNS), which was associated with fungal death by apoptosis. Interestingly, only scavengers of RNS impeded aPI efficacy, suggesting that curcumin acts potently via a nitrosative pathway. The curc-np induced greater NO • expression and enhanced apoptosis of fungal cells, highlighting curc-np aPI as a potential treatment for T. rubrum skin infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by the induction of nitrosative stress after photodynamic activation

2015

Journal of the American Academy of Dermatology

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“…where G is the absorption capacity (M cm 2 ), A is the electrode surface area (cm 2 ), and n, F, y, R, and T are as described in eqn (2). The absorption capacity of CUR on the MIP-CPE surface was calculated to be G ¼ 26.8 nM cm 2 .…”

Section: Reaction Mechanism Studymentioning

confidence: 99%

“…[2][3][4][5][6][7] In traditional Chinese medicine, CUR is believed to help blood circulation, warm the meridians, and cure pain caused by rheumatism and blood stasis.…”

Section: Introductionmentioning

confidence: 99%

A simple and sensitive sensor based on a molecularly imprinted polymer-modified carbon paste electrode for the determination of curcumin in foods

et al. 2017

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A highly sensitive and selective molecularly imprinted polymer-modified carbon paste electrode (MIP-CPE) was designed. The curcumin molecularly imprinted polymer (CUR-MIP) was prepared by thermally induced precipitation polymerization, using curcumin (CUR) as the template molecule, methacrylic acid (MAA) as the functional monomer, 2,2 0 -azodiisobutyronitrile (AIBN) as the initiator, and ethylene glycol dimethacrylate (EDGMA) as the cross-linker. The modified electrode was prepared by mixing the polymer, graphite powder, and paraffin oil in specific proportions. The electrochemical behaviour of CUR on this electrode was studied, and a method for direct CUR determination using this electrode was established. The results showed that CUR exhibited a distinct oxidation peak (E pa ) at 0.434 V (vs. SCE) in the mixed phosphate buffer solution (PBS) at pH 3.06. The optimum addition ratio of the polymer in the modified electrode was 20%, and the peak current (I pa ) was 4.5-fold higher than that of the bare CPE. The I pa showed a good linear relationship with concentration in the range 0.1-50 mM, and the detection limit was 10.1 nM. The method was applied to determine CUR contents in food, giving recovery rates of 90.77-105.7%.

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“…22 Some reports have shown the use of a broad-visible light (400-700 nm) (e.g., halogen lamps, tungsten filament lamps, and white LEDs) to photoinactivate some micro-organisms involved with endodontic infections, 23 dental caries, 24 and mycoses. 25 Overall, these studies used long incubation and exposure times as well.…”

Section: Introductionmentioning

confidence: 99%

Streptococcus MutansPhotoinactivation by Combination of Short Exposure of a Broad-Spectrum Visible Light and Low Concentrations of Photosensitizers

Paschoal

Pinto

Lin

et al. 2014

Photomedicine and Laser Surgery

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Objective: Investigate the photodynamic antimicrobial effect by the combination of a novel noncoherent broad spectrum visible light and low concentrations of curcumin and toluidine blue over suspensions of Streptococcus mutans. Background data: Long illumination times to activate photosensitizers (PS) and the use of high concentrations of these drugs in photodynamic antimicrobial chemotherapy (PACT) are limitations of its application as an antimicrobial technology in dental practice. Materials and methods: Planktonic suspensions of S. mutans were standardized and submitted to PACT treatment at low concentrations of curcumin (C) (0.075; 0.75 and 7.5 lM) and toluidine blue (T) (0.25; 2.5 and 25 lM) exposed to 42 J/cm 2 (12.2 sec; set power: 3.930 mW) of a white light (WL) (output wavelength range: 400-700 nm; beam diameter: 12 mm) (C + WL + and T + WL + , PACT groups; incubation time, C: 60 sec; T: 5 min); isolated effect of both C (C + WL -) and T concentrations (T + WL -); effect of light source (C -WL + and T -WL + ) and suspensions neither submitted to PS nor to light-emitting diode (LED) illumination (control groups, C -WL -and T -WL -). Aliquots of each group were diluted and cultured on blood agar plates and the number of colony-forming units (CFU)/mL was recorded, transformed into log 10 and analyzed by ANOVA and Tukey's test at a cutoff value at 0.05. Results: The groups submitted to PACT presented a bacterial reduction value of > 5-log 10 to both tested PS in comparison with control groups ( p < 0.05). PS or light source used alone demonstrated no antimicrobial effect on the number of viable bacterial counts. Conclusions: The combination of a novel noncoherent light at short illumination exposure time with low concentrations of studied PS achieved a lethal photoinactivation of S. mutans, and can be considered an effective antimicrobial in vitro approach for reducing the number of microorganisms involved with the dental caries process.

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Impact of Curcumin Supersaturation in Antibacterial Photodynamic Therapy—Effect of Cyclodextrin Type and Amount: Studies on Curcumin and Curcuminoides XLV

Cited by 60 publications

References 27 publications

Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by the induction of nitrosative stress after photodynamic activation

Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by the induction of nitrosative stress after photodynamic activation

A simple and sensitive sensor based on a molecularly imprinted polymer-modified carbon paste electrode for the determination of curcumin in foods

Streptococcus MutansPhotoinactivation by Combination of Short Exposure of a Broad-Spectrum Visible Light and Low Concentrations of Photosensitizers

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