Impact of curcumin on replicative and chronological aging in the Saccharomyces cerevisiae yeast

Stępień, Karolina; Wojdyła, Dominik; Nowak, Katarzyna; Mołoń, Mateusz

doi:10.1007/s10522-019-09846-x

Cited by 31 publications

(20 citation statements)

References 46 publications

(63 reference statements)

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“…These antiaging qualities possessed by antioxidant-rich PLME extract from P. longifolia leaf might contribute to extending the RLS of PLME-treated S. cerevisiae BY611 yeast cells, as observed in this study. Similar findings were also reported by Stępień et al (2020) on curcumin, a vegetable-origin bioactive compound that delays replicative aging of the S. cerevisiae wild-type strain BY4741. Indeed, the RLS reported in this study echoes the reproduction potential of S. cerevisiae BY611 yeast cells, but not the actual longevity of the yeast cells studied.…”

Section: Discussionsupporting

confidence: 89%

“…As seen in Figure 1, PLME treatment positively impacted the RLS of S. cerevisiae BY611 yeast cells. The number of daughter cells formed by the mother yeast cell before the cell cycle terminates irreversibly is portrayed as the measurement of life of the yeast cell in the RLS study in a yeast model (Stępień et al, 2020). The quantification of the number of daughter yeast cells formed by a mother yeast cell was done with the aid of a microscope equipped with a micromanipulator to physically separate the daughter cells (Kaeberlein, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of In Situ Antiaging Activity in Saccharomyces cerevisiae BY611 Yeast Cells Treated with Polyalthia longifolia Leaf Methanolic Extract (PLME) Using Different Microscopic Approaches: A Morphology-Based Evaluation

Hemagirri

Sasidharan

2022

Microsc Microanal

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Polyalthia longifolia is known for its anti-oxidative properties, which might contribute to the antiaging action. Hence, the current research was conducted to evaluate the antiaging activity of P. longifolia leaf methanolic extract (PLME) in a yeast model based on morphology using microscopic approaches. Saccharomyces cerevisiae BY611 strain yeast cells were treated with 1.00 mg/mL of PLME. The antiaging activity was assessed by determining the replicative lifespan, total lifespan, vacuole morphology by light microscopy, extra-morphology by scanning (SEM), and intra-morphology by transmission (TEM) electron microscopy. The findings demonstrated that PLME treatment significantly accelerated the replicative and total lifespan of the yeast cells. PLME treatment also delays the formation of large apoptotic-like type 3 yeast cell vacuoles. The untreated yeast cells demonstrated aging morphology via SEM analysis, such as shrinking, regional invaginations, and wrinkled cell surface. The TEM analysis revealed the quintessential aging intracellular morphology such as swollen, wrinkled, or damaged vacuole formation of the circular endoplasmic reticulum, a rupture in the nuclear membrane, fragmentation of the nucleus, and complete damaged cytoplasm. Decisively, the present study revealed the vital role of PLME in the induction of antiaging activity in a yeast model using three microscopic approaches—SEM, TEM, and bright-field light microscope.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Evaluation of In Situ Antiaging Activity in Saccharomyces cerevisiae BY611 Yeast Cells Treated with Polyalthia longifolia Leaf Methanolic Extract (PLME) Using Different Microscopic Approaches: A Morphology-Based Evaluation

Hemagirri

Sasidharan

2022

Microsc Microanal

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“…The total lifespan of the yeast cultures was determined as previously described [ 29 ] with small modifications [ 30 ]. Then, 10 μL aliquots of a fresh yeast culture were collected and transferred to YPD plates with Phloxine B (Fisher Scientific Company, Montreal, QC, Canada) (10 μg/mL) in the medium.…”

Section: Methodsmentioning

confidence: 99%

“…The amount of the produced reactive oxygen species (superoxide anion) was assessed with dihydroethidium (hydroethidine, DHET; final concentration 18.9 μM) (Invitrogen, ThermoFisher Scientific, Waltham, MA USA), as described previously [ 30 ]. Exponentially growing yeast cells were washed with sterile water and diluted to the final density of 10 8 cells/mL in 100 mM phosphate buffer pH 7.0 containing 0.1% ( w / v ) glucose and 1 mM sodium EDTA.…”

Section: Methodsmentioning

confidence: 99%

Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing

Mołoń

Stępień

Kielar

et al. 2022

Cells

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Ageing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in Saccharomyces cerevisiae cells, thus maintaining chromatin dynamics and function, as well as genome stability and cellular morphology. Disrupting this interaction has been proven to influence the stability of the yeast genome and the way cells respond to stress during chronological ageing. It has also been proven that the abrogated interaction between these two chromatin proteins elicited premature ageing phenotypes. Alterations in chromatin compaction have also been associated with replicative ageing, though the main players are not well recognized. Based on this knowledge, here, we examine how the interaction between Hho1p and Arp4p impacts the ageing of mitotically active yeast cells. For this purpose, two sets of strains were used—haploids (WT(n), arp4, hho1Δ and arp4 hho1Δ) and their heterozygous diploid counterparts (WT(2n), ARP4/arp4, HHO1/hho1Δ and ARP4 HHO1/arp4 hho1Δ)—for the performance of extensive morphological and physiological analyses during replicative ageing. These analyses included a comparative examination of the yeast cells’ chromatin structure, proliferative and reproductive potential, and resilience to stress, as well as polysome profiles and chemical composition. The results demonstrated that the haploid chromatin mutants arp4 and arp4 hho1Δ demonstrated a significant reduction in replicative and total lifespan. These findings lead to the conclusion that the importance of a healthy interaction between Arp4p and Hho1p in replicative ageing is significant. This is proof of the concomitant importance of Hho1p and Arp4p in chronological and replicative ageing.

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“…Genetic and metabolic engineering approaches identified factors that delay the onset of aging and cell death in many model species (Stępień et al 2020 ; Gómez-Linton et al 2019 ). Early studies revealed the Sirtuins and TOR (Target of Rapamycin) signaling as longevity regulators (Longo and Kennedy 2006 ; Powers et al 2006 ; Wierman and Smith 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae

et al. 2022

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Molecular causes of aging and longevity interventions have witnessed an upsurge in the last decade. The resurgent interests in the application of small molecules as potential geroprotectors and/or pharmacogenomics point to nicotinamide adenine dinucleotide (NAD) and its precursors, nicotinamide riboside, nicotinamide mononucleotide, nicotinamide, and nicotinic acid as potentially intriguing molecules. Upon supplementation, these compounds have shown to ameliorate aging related conditions and possibly prevent death in model organisms. Besides being a molecule essential in all living cells, our understanding of the mechanism of NAD metabolism and its regulation remain incomplete owing to its omnipresent nature. Here we discuss recent advances and techniques in the study of chronological lifespan (CLS) and replicative lifespan (RLS) in the model unicellular organism Saccharomyces cerevisiae . We then follow with the mechanism and biology of NAD precursors and their roles in aging and longevity. Finally, we review potential biotechnological applications through engineering of microbial lifespan, and laid perspective on the promising candidature of alternative redox compounds for extending lifespan.

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Impact of curcumin on replicative and chronological aging in the Saccharomyces cerevisiae yeast

Cited by 31 publications

References 46 publications

Evaluation of In Situ Antiaging Activity in Saccharomyces cerevisiae BY611 Yeast Cells Treated with Polyalthia longifolia Leaf Methanolic Extract (PLME) Using Different Microscopic Approaches: A Morphology-Based Evaluation

Evaluation of In Situ Antiaging Activity in Saccharomyces cerevisiae BY611 Yeast Cells Treated with Polyalthia longifolia Leaf Methanolic Extract (PLME) Using Different Microscopic Approaches: A Morphology-Based Evaluation

Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing

The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae

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