Impact of Culture Filtrate of Piriformospora indica on Biomass and Biosynthesis of Active Ingredient Aristolochic Acid in Aristolochia elegans Mart

Abstract

The mycorrhiza plant partnership is the basic, essential and integral part of plant survival and growth. In the present investigation we are reporting the effect of culture filtrate of Piriformospora indica, a growth promoter and bioprotector fungus on Aristolochia elegans Mart. The culture filtrate of the fungus increased overall growth, biomass, and active ingredient-aristolochic acid in the leaves of plants. In untreated control plants, the overall growth was reduced. P. indica culture filtrate application increased root number, root length, and root dry weightby 28%, 98%, and 123% respectivelyin plants of Aristolochia. Also stem height and shoot length was enhanced by 43% and 155% respectively.There was increase in number of leaves by 79% and length of leaves by 36%. The increase in total biomass was 136%. The improvement in content of aristolochic acid in leaves was between7.6% and 28.8%in treated plants as against untreated control plants

Piriformospora indica: Potential and Significance in Plant Stress Tolerance

“…This fungus has been reported to perform multifarious functions, including its role in biological hardening during transplantation of micro-propagated plantlets ( Singh et al, 2003 ), increased endogenous content of spilanthol after realization of its mutual interaction with medicinal plants such as Spilanthes calva ( Rai et al, 2004 ). P. indica infestation in Helianthus annus and Aristolochia elegans has resulted into the stimulated synthesis of valuable compounds ( Bagde et al, 2010a , 2014 ). Additionally, Bacopa monnieri co-cultivated with P. indica exhibited an enhanced growth, elevated bacoside endogenous level, antioxidant activity and nuclear hypertrophy ( Prasad et al, 2013 ).…”

Influence of the root endophyte Piriformospora indica on the plant water relations, gas exchange and growth of Chenopodium quinoa at limited water availability

“…P. indica inoculation was found to improve tremendously the growth and biomass of diverse hosts including Oryza sativa, Triticum sativum, Zea mays, Setaria italica, Sorghum vulgare (Achatz et al., ; Baltruschat et al., ; Varma, Rai, & Sahay, ; Varma, Verma, Sudah, & Franken, ), Arabidopsis thaliana (Peškan‐Berghöfer et al., ; Shahollari, Varma, & Oelmüller, ; Sherameti et al., ), Hordeum vulgare (Achatz et al., ; Baltruschat et al., ; Waller et al., , ), Nicotiana attenuata (Barazani, Dahl, & Baldwin, ), Brassica chinensis (Sun et al., ) and Lycopersicon esculentum (Sarma et al., ; Trivedi et al, ,b). Its promoting effect may also be traced down at the biochemical level with increased production of certain phytochemicals and secondary metabolites in several medicinal and industrial crops (Bagde, Prasad, & Varma, , ). Furthermore, several lines of evidence have supported the role P. indica symbiosis in enhancing drought stress resistance in plant species such as barley (Waller et al., ), arabidopsis (Sherameti, Tripathi et al., ; Sherameti, Venus et al., ), chinese cabbage (Sun et al., ) and maize (Xu, Wang, Wang, Wei, & Zhang, ).…”

Piriformospora indica: a root endophytic fungus and its roles in plants

“…The culture filtrate of P. indica also stimulated plant growth (Varma et al, 2001;Sharma et al, 2013;Bagde et al, 2014). Bagde et al (2014) used the culture filtrate of P. indica into Aristolochia elegans, and found the enhancement in root number and length, plant height, leaf number, and plant biomass.…”

“…The culture filtrate of P. indica also stimulated plant growth (Varma et al, 2001;Sharma et al, 2013;Bagde et al, 2014). Bagde et al (2014) used the culture filtrate of P. indica into Aristolochia elegans, and found the enhancement in root number and length, plant height, leaf number, and plant biomass. Culture filtrate of P. indica also promoted hairy root biomass of Vigna mungo (Bagde et al, 2014) and plant growth of Artemisia annua (Sharma et al, 2013), flax plants , and sunflower (Bagde et al, 2011).…”

“…Promotion of bioactive substance accumulation P. indica has a critical role in promoting bioactive substance accumulation of plants. The aristolochic acid concentrations in leaves of Aristolochia elegans were heavily induced by the fungal culture filtrate (Bagde et al, 2014), indicating an important role in accelerating bioactive substances. In Artemisia annua, the culture filtrate of P. indica comparatively increased artemisinin levels, an antimalarial compound (Sharma et al, 2013).…”