Impact of cross-breeding of low phytic acid rice (Oryza sativa L.) mutants with commercial cultivars on the phytic acid contents

Zhou, Chenguang; Tan, Yuanyuan; Goßner, Sophia; Li, Youfa; Shu, Qingyao; Engel, Karl‐Heinz

doi:10.1007/s00217-018-3192-3

Cited by 5 publications

(10 citation statements)

References 23 publications

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“…They also observed significantly lower vigour index in all mutant lines as compared to their parents, with reduction of 7.8%–26.3%. However, it has been reported that grain yield and field emergence can be improved through cross and selection breeding in rice (Zhou et al, 2019). Cross breeding and marker assisted selection were also used in soybean to improve germination and yield of low phytate mutants (Spear and Fehr, 2007) and indeed two barley Lpa cultivars, Clearwater, and CDC Lophy-1, have already been released for commercial production (Bregitzer et al, 2008; Rossnagel et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Primary LPA mutants often feature inferior agronomic performance in terms of field emergence and grain yield (Zhao et al, 2008a; Raboy, 2009). Different studies have demonstrated that mutations in phytic acid genes impairs plant growth, lower grain yield, and reduce seed viability compared with wild type (Rutger et al, 2004; Jiang et al, 2019; Zhou et al, 2019). It has also been reported that germination and grain yield can be improved through breeding and selection (Spear and Fehr, 2007); however, extensive efforts are needed in breeding LPA rice cultivars for commercial production.…”

Section: Introductionmentioning

confidence: 99%

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Development and Molecular Characterization of Low Phytate Basmati Rice Through Induced Mutagenesis, Hybridization, Backcross, and Marker Assisted Breeding

et al. 2019

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Breeding low phytate crops is the most viable solution to tackle mineral deficiencies. The objective of the present study was to develop high yielding, low phytate (lpa) basmati rice cultivars. Three homozygous lpa mutants, Lpa5, Lpa9, and Lpa59, were developed through induced mutations (gamma rays 60Co) and identified by colorimetric and High Performance Liquid Chromatography (HPLC) analysis. These mutants showed 54%–63% reduction in phytic acid but had poor germination and yield. To improve these traits, hybridization and back cross breeding involving Lpa5, Lpa59, and parent cultivar Super Basmati were performed and F2:3, F3:4, BC1F2:3, and BC1F3:4 generations were developed and screened to target the objective. Within the F2:3, homozygous (226), heterozygous (65), and wild type (46) lpa recombinants were identified. Within the homozygous lpa category, four recombinants (Lpa5, Lpa6, Lpa7, and Lpa30) showed improved germination. Within the F3:4 generation, 86 homozygous lpa recombinants were identified. Further selection, on the basis of better plant type and the low phytate trait resulted in the selection of 38 recombinants. Grain quality and cooking characteristics of these selected recombinants were comparable as compared to parent cultivar. Within the BC1F2:3 generations, two homozygous Lpa recombinant lines, Lpa141, and Lpa205, were selected out of 220. Screening of the BC1F3:4 generation for the desirable agronomic and low phytate trait also resulted in the selection of two homozygous lines. Finally, seven recombinants i.e. Lpa12-3, Lpa111-1, Lpa141, Lpa56-3, Lpa53-4, Lpa99-2, and Lpa205-4 out of 42 homozygous low phytate lines were selected on the basis of yield improvement (4%–18%) as compared to parent cultivar. Association analysis suggested that further selection based on primary branches per plant, panicle length and productive tillers per plant would further improve the paddy yield. For molecular characterization of the Lpa trait, previously reported Lpa1-CAPS and Lpa1-InDel and functional molecular markers were applied. Results indicated the absence of the Z9B-Lpa allele and XS-Lpa mutation in the OsMRP5 gene in tested mutants, possibly suggesting that there may be new mutations or novel alleles in tested mutants that need to be identified and then fine mapped for subsequent utilization. To our knowledge, this is the first report of low phytic acid rice mutant development and their improved germination and yield through backcross breeding in basmati rice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Molecular Characterization of Low Phytate Basmati Rice Through Induced Mutagenesis, Hybridization, Backcross, and Marker Assisted Breeding

et al. 2019

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“…The phytic acid contents of the rice seed samples were determined via high-pressure ion chromatography using an ultraviolet detector at 290 nm, as previously described …”

Section: Methodsmentioning

confidence: 99%

“…To minimize these adverse effects, various low phytic acid ( lpa ) crops, for example, maize, barley, rice, and soybean, have been developed via techniques such as genetic engineering and mutation breeding . Recently, it has been demonstrated that cross and selection breeding of lpa rice mutants with commercial rice cultivars could be employed as a strategy to obtain progeny rice seeds stably expressing the intended lpa trait . However, the impact of cross-breeding of two lpa rice mutants on the phytic acid contents in the resulting progenies has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Phytic Acid Contents and Metabolite Profiles of Progenies from Crossing Low Phytic Acid OsMIK and OsMRP5 Rice (Oryza sativa L.) Mutants

Tan

Zhou

Goßner

et al. 2019

J. Agric. Food Chem.

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The impact of cross-breeding two low phytic acid (lpa) rice mutants on the content of phytic acid and the metabolite profile of the resulting double mutant was investigated. Progenies resulting from the cross of Os-lpa-XS110-1, a rice mutant carrying the myo-inositol kinase (OsMIK) mutated gene, and Os-lpa-XS110-2, with the multidrug resistance-associated protein ABC transporter gene 5 (OsMRP5) as the mutation target, were subjected to high-pressure ion chromatography. The reduction of the phytic acid content in the double mutant (−63%) was much more pronounced than in the single mutants (−26 and −47%). Gas chromatography-based metabolite profiling revealed a superimposition of the metabolite profiles inherited from the lpa progenitors in the double mutant progenies; the resulting metabolite signature was predominated by the OsMIK mutation effect. The study demonstrated that cross-breeding of two single lpa mutants can be employed to generate double lpa rice mutants showing both a significant reduction in the content of phytic acid and the imprinting of a specific mutation-induced metabolite signature.

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“…Low phytic acid (LPA) rice mutants were developed to overcome this problem, but these lines presented different agronomic penalties associated with low grain yield [ 31 , 42 ]. Different mutations in genes involved in the biosynthesis of PA were shown to hinder plant growth and reduce seed viability [ 32 , 43 , 44 ]. Still, other studies showed that different types of breeding (mutagenesis, hybridization, backcross, and marker-assisted breeding) and selection programs can be employed to improve the germination traits in LPA mutants [ 45 ].…”

Section: Linking Rice Biofortification With Seed Germinationmentioning

confidence: 99%

Transcriptomics View over the Germination Landscape in Biofortified Rice

Dueñas

Slamet‐Loedin

Macovei

2021

Genes

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Hidden hunger, or micronutrient deficiency, is a worldwide problem. Several approaches are employed to alleviate its effects (e.g., promoting diet diversity, use of dietary supplements, chemical fortification of processed food), and among these, biofortification is considered as one of the most cost-effective and highly sustainable. Rice is one of the best targets for biofortification since it is a staple food for almost half of the world’s population as a high-energy source but with low nutritional value. Multiple biofortified rice lines have been produced during the past decades, while few studies also reported modifications in germination behavior (in terms of enhanced or decreased germination percentage or speed). It is important to underline that rapid, uniform germination, and seedling establishment are essential prerequisites for crop productivity. Combining the two traits, biofortified, highly-nutritious seeds with improved germination behavior can be envisaged as a highly-desired target for rice breeding. To this purpose, information gathered from transcriptomics studies can reveal useful insights to unveil the molecular players governing both traits. The present review aims to provide an overview of transcriptomics studies applied at the crossroad between biofortification and seed germination, pointing out potential candidates for trait pyramiding.

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Impact of cross-breeding of low phytic acid rice (Oryza sativa L.) mutants with commercial cultivars on the phytic acid contents

Cited by 5 publications

References 23 publications

Development and Molecular Characterization of Low Phytate Basmati Rice Through Induced Mutagenesis, Hybridization, Backcross, and Marker Assisted Breeding

Development and Molecular Characterization of Low Phytate Basmati Rice Through Induced Mutagenesis, Hybridization, Backcross, and Marker Assisted Breeding

Phytic Acid Contents and Metabolite Profiles of Progenies from Crossing Low Phytic Acid OsMIK and OsMRP5 Rice (Oryza sativa L.) Mutants

Transcriptomics View over the Germination Landscape in Biofortified Rice

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