Impact of Cost-Sharing on Preventive Health Services in the United States: A Critical Review

Johnson, Kelly; Newransky, Chrisann; Zhang, Dongmu; Kawai, Kosuke; Acosta, Camilo J.

doi:10.1093/ofid/ofv133.98

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“…Virulence genes. Using multiplex PCR, the isolates were tested for 52 virulence genes associated with ExPEC (40). Isolates were classified as ExPEC if positive for Ն2 of the following genes: papA and/or papC (P fimbriae; counted as one), sfa or focDE (S and F1C fimbriae), afa or draBC (Dr-binding adhesins), kpsMII (group 2 capsule synthesis), and iutA (aerobactin system) (41).…”

Section: Methodsmentioning

confidence: 99%

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla _CTX-M-27 or bla _CTX-M-15

Jamborová

Johnston

Papoušek

et al. 2018

Antimicrob Agents Chemother

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sequence type 131 (ST131) is currently one of the leading causes of multidrug-resistant extraintestinal infections globally. Here, we analyzed the phenotypic and genotypic characteristics of 169 ST131 isolates from various sources (wildlife, wastewater, companion animals, community, and hospitals) to determine whether wildlife and the environment share similar strains with humans, supporting transmission of ST131 between different ecological niches. Susceptibility to 32 antimicrobials was tested by disc diffusion and broth microdilution. Antibiotic resistance genes, integrons, plasmid replicons, 52 virulence genes, and -based subtypes were detected by PCR and DNA sequencing. Genomic relatedness was determined by pulsed-field gel electrophoresis (PFGE). The genetic context and plasmid versus chromosomal location of extended-spectrum beta-lactamase and AmpC beta-lactamase genes was determined by PCR and probe hybridization, respectively. The 169 ST131 study isolates segregated predominantly into30Rx (60%) and 30R1 (25%) subclones. Within each subclone, isolates from different source groups were categorized into distinct PFGE clusters; genotypic characteristics were fairly well conserved within each major PFGE cluster. Irrespective of source, the30Rx isolates typically exhibited virotype A (89%), an F2:A1:B- replicon (84%), and a 1.7-kb class 1 integron (92%) and had diverse structures upstream of the region. In contrast, the30R1 isolates typically exhibited virotype C (86%), an F1:A2:B20 replicon (76%), and a conserved IS-ΔIS-like structure. Despite considerable overall genetic diversity, our data demonstrate significant commonality between ST131 isolates from diverse environments, supporting transmission between different sources, including humans, environment, and wildlife.

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Section: Methodsmentioning

confidence: 99%

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla _CTX-M-27 or bla _CTX-M-15

Jamborová

Johnston

Papoušek

et al. 2018

Antimicrob Agents Chemother

View full text Add to dashboard Cite

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Impact of Cost-Sharing on Preventive Health Services in the United States: A Critical Review

Cited by 1 publication

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Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla _CTX-M-27 or bla _CTX-M-15

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla _CTX-M-27 or bla _CTX-M-15

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Impact of Cost-Sharing on Preventive Health Services in the United States: A Critical Review

Cited by 1 publication

References 0 publications

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla CTX-M-27 or bla CTX-M-15

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla CTX-M-27 or bla CTX-M-15

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Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla _CTX-M-27 or bla _CTX-M-15

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 ( H 30R1 and H 30Rx Subclones) That Carry bla _CTX-M-27 or bla _CTX-M-15