Impact of copper oxide nanoparticles (CuO NPs) exposure on embryo development and expression of genes related to the innate immune system of zebrafish (Danio rerio)

Aksakal, Feyza İçoğlu; Çiltaş, Abdülkadir

doi:10.1016/j.cbpc.2019.05.016

Cited by 46 publications

(35 citation statements)

References 57 publications

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“…Especially, it was showed that CuO NPs have serious teratogenic effects on zebrafish embryos. 0.15‐1.0 mg/l CuO NPs induced tail flexure, spinal curvature, yolk sac edema, notochord and head malformation in 96 hpf zebrafish embryos 22 . Similar results were found in 48 hpf embryos at 5‐120 ppm CuO NPs 37 .…”

Section: Discussionsupporting

confidence: 72%

“…0.15-1.0 mg/l CuO NPs induced tail flexure, spinal curvature, yolk sac edema, notochord and head malformation in 96 hpf zebrafish embryos. 22 Similar results were found in 48 hpf embryos at 5-120 ppm CuO NPs. 37 The exposure to Ag, ZnO, and CuO NPs also reduced hatching success.…”

Section: Gene Expressionsupporting

confidence: 73%

“…In this study, exposure to CNPE has led to an increase in hsp70 gene expression and a decrease in mtf‐1 gene expression. Similarly, the increase in hsp70 gene expression and the decrease in mtf‐1 gene expression were observed in zebrafish exposed to copper oxide and silver nanoparticles 22,58 …”

Section: Discussionmentioning

confidence: 93%

“…21 The exposure concentrations of CNPE were 1.0, 2.0, 4.0, 8.0, and 16.0 mg/l, and the exposure time was 96 hpf. The selection of exposure concentration was based on Aksakal, Arslan 19 Aksakal, Ciltas, 22 and our preliminary tests. Thirtyfour hpf embryos were randomly selected for each experimental group and control.…”

Section: Developmental Toxicity Testmentioning

confidence: 99%

“…Similarly, the increase in hsp70 gene expression and the decrease in mtf-1 gene expression were observed in zebrafish exposed to copper oxide and silver nanoparticles. 22,58 At the embryonic stage, proliferation, differentiation, growth and basal metabolism are important for embryo development. Their disruption by various chemicals can lead to the emergence of toxic effects in the embryo.…”

Section: Gene Expressionmentioning

confidence: 99%

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Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Aksakal

Şi̇şman

2020

Environmental Toxicology

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The current study evaluates the adverse effects of Cu(OH)2 nanopesticide (CNPE) on the early life stages of zebrafish (Danio rerio). The developmental toxicity was determined using different parameters such as mortality (including LC50), hatching, heart rates, malformations, and alteration of the gene expressions. Zebrafish embryos (4 hpf‐hours postfertilization) were exposed to 1.0, 2.0, 4.0, 8.0, and 16.0 mg/l CNPE doses until 96 hpf. The 96 hours LC50 was recorded at 6.258 mg/l. Seventy‐two hpf total malformation index values for 2.0, 4.0, and 8.0 mg/l CNPE doses were 4.3, 7.2 and 7.9, respectively. 1.0 mg/l CNPE is not toxic for the zebrafish embryos/larvae. 2.0 to 8.0 CNPE doses caused some abnormalities in embryos/larvae morphology, including lack of body parts, tail deformities, chorda deformity, bubbled head, scoliosis, lordosis, weak or non‐pigmentation, decreased heart rate and larva length. 16.0 mg/l CNPE caused mortality in 72 hpf. The expression levels of seven immune system‐related genes (il‐1β, il‐8, cebp, tlr4, hsp70, NF‐kB, and mtf‐1) were examined. The transcription level of il‐1β, il‐8, tlr4, hsp70, and NF‐kB genes significantly increased in the CNPE exposure groups. While the expression of the mtf‐1 gene considerably decreased, the cebp gene expression level did not change in the 4.0 and 8.0 mg/l CNPE doses. In conclusion, CNPE could induce developmental toxicity with malformations in embryos/larvae and alter the gene expression.

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Section: Discussionsupporting

confidence: 72%

Section: Gene Expressionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 93%

Section: Developmental Toxicity Testmentioning

confidence: 99%

Section: Gene Expressionmentioning

confidence: 99%

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Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Aksakal

Şi̇şman

2020

Environmental Toxicology

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Characterization of Genomic and Epigenomic Biomarkers of Nanoparticle Toxicity Using the Zebrafish Model System

Suku

Mohanan

Freeman

2022

Genomic and Epigenomic Biomarkers of Toxicology and Disease

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Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Demir

2022

J of Applied Toxicology

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Boron trioxide nanoparticles (B 2 O 3 NPs) have recently been widely used in a range of applications including electronic device technologies, acousto-optic apparatus fields, and as nanopowder for the production of special glasses. We propose Drosophila melanogaster as a useful in vivo model system to study the genotoxic risks associated with NP exposure. In this study, we have conducted a genotoxic evaluation of B 2 O 3 NPs (size average 55.52 ± 1.41 nm) and its ionic form in D. melanogaster. B 2 O 3 NPs were supplied to third instar larvae at concentrations ranging from 0.1-10 mM. Toxicity, intracellular oxidative stress (reactive oxygen species, ROS), phenotypic alterations, genotoxic effect (via the wing somatic mutation and recombination test, SMART), and DNA damage (via Comet assay) were the end-points evaluated. B 2 O 3 NPs did not cause any mutagenic/recombinogenic effects in all tested non-toxic concentrations in Drosophila SMART. Negative data were also obtained with the ionic form. Exposure to B 2 O 3 NPs and its ionic form (at two highest concentrations, 2.5 and 5 mM) was found to induce DNA damage in Comet assay. Additionally, ROS induction in hemocytes and phenotypic alterations were determined in the mouths and legs of Drosophila. This study is the first study reporting genotoxicity data in the somatic cells of Drosophila larvae, emphasizing the importance of D. melanogaster as a model organism in investigating the different biological effects in a concentrationdependent manner caused by B 2 O 3 NPs and its ionic form. The obtained in vivo results contribute to improvement the genotoxicity database on the B 2 O 3 NPs.

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Impact of copper oxide nanoparticles (CuO NPs) exposure on embryo development and expression of genes related to the innate immune system of zebrafish (Danio rerio)

Cited by 46 publications

References 57 publications

Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Characterization of Genomic and Epigenomic Biomarkers of Nanoparticle Toxicity Using the Zebrafish Model System

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

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Impact of copper oxide nanoparticles (CuO NPs) exposure on embryo development and expression of genes related to the innate immune system of zebrafish (Danio rerio)

Cited by 46 publications

References 57 publications

Developmental toxicity induced by Cu(OH)2 nanopesticide in zebrafish embryos

Developmental toxicity induced by Cu(OH)2 nanopesticide in zebrafish embryos

Characterization of Genomic and Epigenomic Biomarkers of Nanoparticle Toxicity Using the Zebrafish Model System

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

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Product

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Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos