Impact of copper on the diversity, abundance and transcription of nitrite and nitrous oxide reductase genes in an urban European estuary

Magalhães, Catarina; Machado, Ana; Matos, Pedro; Bordalo, Adriano A.

doi:10.1111/j.1574-6941.2011.01107.x

Cited by 84 publications

(61 citation statements)

References 56 publications

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“…Numerous studies have examined the factors that influence different pathways involved in denitrification, such as nitrate (Magalhães et al 2005), carbon availability (Henderson et al 2010), pH (Liu et al 2010), and copper concentration (Magalhães et al 2011;Felgate et al 2012). Nevertheless, compaction, as a physical factor, has not received as much attention as the above chemical factors associated with denitrification.…”

Section: Introductionmentioning

confidence: 99%

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Deng

Rensing

et al. 2013

Environ Sci Pollut Res

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Soils in urban areas are subjected to compaction with accelerating urbanization. The effects of anthropogenic compaction on urban soil denitrification are largely unknown. We conducted a study on an urban park soil to investigate how compaction impacts denitrification. By using 15 N labeling method and acetylene inhibition technique, we performed three coherent incubation experiments to quantify denitrification in compacted soil under both aerobic and anaerobic conditions. Uncompacted soil was set as the control treatment. When monitoring soil incubation without extra substrate, higher nitrous oxide (N 2 O) flux and denitrification enzyme activity were observed in the compacted soil than in the uncompacted soil. In aerobic incubation with the addition of K 15 NO 3 , N 2 O production in the compacted soil reached 10.11 ng N h −1 g −1 as compared to 0.02 ng N h −1 g −1 in the uncompacted soil. Denitrification contributed 96 % of the emitted N 2 O in the compacted soil and 36 % of the emitted N 2 O in the uncompacted soil; total denitrification rate was higher in the compacted soil (up to 79.35 ng N h −1 g −1 ) than in the uncompacted soil (0.11 ng N h −1 g −1 ). Under anaerobic incubation with the addition of K 15 NO 3 , no statistical difference in total N losses and 15 N-(N 2 O+N 2 ) flux between the uncompacted soil and the compacted soil was detected. Compaction promoted soil denitrification and may impact urban N biogeochemical cycling.

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Section: Introductionmentioning

confidence: 99%

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Deng

Rensing

et al. 2013

Environ Sci Pollut Res

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“…There are evidences that metals may be an important factor in regulating nitrogen transformations in sediment habitats. For example, in the Douro River estuary (north-west Portugal), the transcription diversity of the nosZ gene showed a drastic decrease with the increase of Cu concentration (Magalhães et al 2011). The metal toxicity was modulated by sediment properties (metal concentrations, grain size, organic carbon to nitrogen ratio, etc.…”

Section: Introductionmentioning

confidence: 99%

Contrasting spatiotemporal patterns and environmental drivers of diversity and community structure of ammonia oxidizers, denitrifiers, and anammox bacteria in sediments of estuarine tidal flats

et al. 2014

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The spatial and temporal patterns of diversity, community structure, and their drivers are fundamental issues in microbial ecology. This study aimed to investigate the relative importance of spatial and seasonal controls on the distribution of nitrogen cycling microbes in sediments of estuarine tidal flats, and to test the hypothesis that metals impact the distribution of nitrogen-cycling microbes in the coastal system. Two layers of sediment samples were collected from three estuarine tidal flats of Laizhou Bay in 2010 winter and 2011 summer. The alpha diversities (Shannon and Simpson indices) and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA), denitrifier and anammox bacteria (AMB) were revealed using denaturing gradient gel electrophoresis and clone library analysis of amoA, nosZ and 16S rRNA gene markers. We found that both AOB and AMB exhibited distinct seasonal patterns in either alpha diversity or community turnover; AOA had different alpha diversities in two layers, but neither spatial nor seasonal patterns were found for their community turnover. However, no distinct spatiotemporal pattern was observed for either diversity or community composition of nosZ-type denitrifiers. For correlations between alpha diversities and environmental factors, significant correlations were found between AOB and ammonium, temperature and As, between denitrifiers and nitrite, salinity and Pb, and between AMB and Pb, ratio of organic carbon to nitrogen, ammonium, pH and dissolved oxygen. Salinity and sediment grain size were the most important factors shaping AOB and AOA communities, respectively; whereas AMB community structure was mostly determined by temperature, dissolved oxygen, pH and heavy metals As and Cd. These results stress that ammonia oxidizers, denitrifiers and anammox bacteria have generally different distributional patterns across time and space, and heavy metals might have contributed to their differentiated distributions in coastal sediments.

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“…Moreover, reduced sulfur compounds might be oxidized to sulfuric acid if sufficient oxygen is introduced by the plants (Stubner et al 1998), thereby a lower pH in the vegetated sediments was observed. Several studies have illustrated that soil or sediment physiochemical properties are a major factor group influencing the activity, size, and community of denitrifiers (Perryman et al 2011;Bañeras et al 2012;Guo et al 2013;Li et al 2013). The changes in the sediment properties caused by these factors play an important role in affecting the denitrifier activities and abundances, thus further shaping the denitrifier community compositions.…”

Section: Discussionmentioning

confidence: 98%

Impacts of vegetation, tidal process, and depth on the activities, abundances, and community compositions of denitrifiers in mangrove sediment

Wang

Zheng

et al. 2014

Appl Microbiol Biotechnol

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Coastal mangrove wetland is well known to be intense in nitrogen cycling. The tidal process and plants are key factors in controlling the microbial processes in wetlands. However, mechanisms on how these factors and their interactions affect the microorganisms involved in denitrification remain poorly understood. In this study, the impacts of vegetation (bulk, Kandelia obovata, and Spartina alterniflora) and tidal process (falling tide and rising tide) on denitrification activities, abundances, and community compositions of denitrifiers in the sediments from different depths (0-5 and 5-10 cm) were investigated in a microcosm experiment. A significant enhancement of denitrification activities and gene abundances (nirS, nirK, and nosZ) in the vegetated sediments was observed. Activities and abundances were significantly higher in the 0-5-cm sediments when compared with the 5-10-cm counterparts. The effect of interaction between vegetation and tide or depth was also significant. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed that not only vegetation but also plant species had a significant impact on the community compositions of nirK denitrifiers, while the tidal process affected the community compositions of nirS and nosZ denitrifiers but not nirK denitrifiers. However, depth only significantly shaped the nirS denitrifier communities. These findings demonstrate the effects of these factors and their interactions in shaping the denitrifiers in sediments.

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Impact of copper on the diversity, abundance and transcription of nitrite and nitrous oxide reductase genes in an urban European estuary

Cited by 84 publications

References 56 publications

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Contrasting spatiotemporal patterns and environmental drivers of diversity and community structure of ammonia oxidizers, denitrifiers, and anammox bacteria in sediments of estuarine tidal flats

Impacts of vegetation, tidal process, and depth on the activities, abundances, and community compositions of denitrifiers in mangrove sediment

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