Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves

Rocchetti, Gabriele; Blasi, Francesca; Montesano, Domenico; Ghisoni, Silvia; Marcotullio, Maria Carla; Sabatini, Stefano; Cossignani, Lina; Lucini, Luigi

doi:10.1016/j.foodres.2018.11.046

Cited by 126 publications

(88 citation statements)

References 36 publications

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“…Regarding antioxidant potential (FRAP and ORAC) and the relationship with phenolic compounds, the results obtained are in agreement with those previously reported by other authors who observed that, in a complex matrix like tomato, tocopherols, ascorbic acid, and carotenoids seem to be the main contributors to total antioxidant activity . Moreover, as measured by the FRAP and ORAC assays, correlations between the polyphenol profile and the in vitro antioxidant activity values were observed.…”

Section: Discussionmentioning

confidence: 99%

Untargeted screening of the bound / free phenolic composition in tomato cultivars for industrial transformation

et al. 2019

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BACKGROUND Tomato is one of the most important agricultural crops and it is characterized by a wide bioactive compound profile. However, little information is reported on its comprehensive polyphenol profile. In this work, 13 commercial tomato cultivars for industrial transformation were screened by ultra‐high‐pressure liquid chromatography coupled to quadrupole‐time‐of‐flight mass spectrometry (UHPLC‐QTOF‐MS) for both free and bound phenolic profiles. Thereafter, the in vitro antioxidant activity of each cultivar was assessed by ferric reducing antioxidant power (FRAP) and oxygen radical absorbance activity (ORAC) assays. Multivariate statistics, i.e. orthogonal projection to latent structures discriminant analysis (OPLS‐DA), were then used to model samples according to their distinct phenolic signatures, thus providing compounds that better discriminated between the distributions of the cultivars that were considered. RESULTS More than 350 phenolic compounds could be identified across the samples that were considered: flavonoids (such as flavones and flavanols), hydroxycinnamic acids, lignans, and lower‐molecular‐weight phenolics were the most frequently observed classes of phenolics in tomato berries. Anthocyanins were the most abundant class among bound phenolics (being highest in the Leader F1 and Defender F1 cultivars), followed by tyrosols (mainly in Heinz cultivars). However, flavones and hydroxybenzoic acids were the most represented discriminant phenolics in the bound fraction. CONCLUSIONS Untargeted metabolomics allowed significant differences in phenolic composition to be outlined across the tomato cultivars that were analyzed. Such differences were particularly evident regarding the free‐to‐bound phenolic ratio, hence allowing differences in the bioaccessibility of phenolics to be postulated. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Untargeted screening of the bound / free phenolic composition in tomato cultivars for industrial transformation

et al. 2019

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“…The untargeted phenolic profiling of the different Crataegus extracts was investigated by means of UHPLC (Agilent 1290 HPLC liquid chromatograph) coupled to a QTOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). The experimental conditions for the analysis of plant extracts by means of untargeted metabolomics were optimized as described in previous work . The mass spectrometer acquired ions in the range 50–1100 m / z in positive scan mode.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the Agilent Profinder B.06 software was used for post‐acquisition data filtering, retaining only those compounds identified within 100% of replications in at least one condition. Finally, to provide more quantitative information, phenolic compounds were first ascribed into classes and subclasses, and then were quantified using standard solutions (80/20, v/v methanol/water) of pure standard phenolic compounds analysed with the same method . The following phenolic classes were targeted: anthocyanins (quantified as cyanidin equivalents), flavones (quantified as luteolin equivalents), flavonols and flavanols (quantified as catechin equivalents), lignans (quantified as sesamin equivalents), alkylphenols (quantified as 5‐pentadecylresorcinol equivalents), stilbenes (quantified as resveratrol equivalents), low‐molecular‐weight phenolics (quantified as tyrosol equivalents), and phenolic acids (quantified as ferulic acid equivalents).…”

Section: Methodsmentioning

confidence: 99%

“…A one‐way analysis of the variance (ANOVA) was performed considering data from each assay and using the software PASW Statistics 25.0 (SPSS Inc.), followed by a Duncan's post hoc test ( P > 0.05). Afterwards, the metabolomics‐based data set exported from Mass Profiler Professional B.12.06 (Agilent Technologies, Santa Clara, CA, USA) was transferred into a second software package, namely SIMCA 13 (Umetrics, Malmo, Sweden), for supervised orthogonal projections to latent structures discriminant analysis (OPLS‐DA), according to previously reported work . The variable importance in projection (VIP) selection method was used in order to evaluate the importance of each phenolic compound in discriminating the different extraction techniques, and to select those having the highest discrimination potential (VIP score >1).…”

Section: Methodsmentioning

confidence: 99%

“…The experimental conditions for the analysis of plant extracts by means of untargeted metabolomics were optimized as described in previous work. 29,30 The mass spectrometer acquired ions in the range 50-1100 m/z in positive scan mode. The database Phenol-Explorer (http://phenol-explorer.eu) was then used for annotation purposes, exploiting the entire isotopic profile (i.e.…”

Section: Untargeted Metabolomics-based Profiling Of Polyphenolsmentioning

confidence: 99%

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Untargeted metabolomic profiling of three Crataegus species (hawthorn) and their in vitro biological activities

et al. 2020

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BACKGROUND In this investigation, the chemical and biological profiles of three Turkish Crataegus species (Crataegus orientalis, Crataegus szovitsii and Crataegus tanacetifolia) were studied in order to provide the first comprehensive characterization and their health‐promoting potential. In this respect, polyphenolic profiles were evaluated using ultrahigh‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry. The in vitro antioxidant activities of the Crataegus samples were evaluated by using free‐radical scavenging, phosphomolybdenum, ferrous‐ion chelating, and reducing power assays. The inhibitory activities against α‐glucosidase, amylase, cholinesterases (acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)), and tyrosinase were also established. RESULTS The untargeted metabolomic approach highlighted the effect of both extraction types and species on the phenolic profiles of different Crateagus tissues (i.e. leaves and twigs). The methanolic extracts showed the maximum radical scavenging and reducing activity in all test systems, whereas for ferrous‐ion chelating assays the decocted and infused extracts showed the highest activity. Only the methanolic extracts were effective against AChE and BChE. The extract tested showed remarkable inhibitory effects against tyrosinase and α‐glucosidase, whereas all the extracts exhibited modest inhibition against α‐amylase. Overall, the twig extracts of the three species studied showed superior antioxidant and enzyme inhibitory activities. CONCLUSION On the basis of these results, the three Crataegus species can be classified as potent bioresources for high‐value phytochemicals, which warrant further investigations for developing novel nutraceuticals. © 2019 Society of Chemical Industry

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Oxidative stability, structural, and textural properties of margarine enriched with Moringa oleifera leaves extract

Ouahrani

Tzompa‐Sosa

Dewettinck

et al. 2022

J Americ Oil Chem Soc

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Consumer's demand for clean label food ingredients has driven the development of alternative food additives. This study falls within this challenge through valorization of Moringa oleifera leaves grown in Algeria as a natural antioxidant. A methanolic M. oleifera leaves extract (MOLE) was prepared and included in margarine at various levels (400, 600, and 800 ppm) and was compared to vitamin E and a margarine without antioxidants. The effect of addition of MOLE on margarine quality was studied by means of its oxidative stability, structural, textural properties, color, and aroma fingerprint. It was shown that addition of MOLE to margarine increased resistance towards oxidation, showing a higher antioxidant capacity as compared to margarine with vitamin E or without antioxidants. Moreover, addition of MOLE decreased water droplet size, which is desirable from a microbiological viewpoint since it could extend margarine's shelf life. Furthermore, addition of MOLE leads to the formation of smaller fat crystals, resulting in different fat network formation, which could be the cause of the increase in hardness seen in these margarines. Regarding sensorial effects, MOLE addition led to a significant darkening of the margarine and increase yellowness. Moreover, the aroma fingerprint showed that addition of MOLE modified the aroma fingerprint of this product.

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Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves

Cited by 126 publications

References 36 publications

Untargeted screening of the bound / free phenolic composition in tomato cultivars for industrial transformation

Untargeted screening of the bound / free phenolic composition in tomato cultivars for industrial transformation

Untargeted metabolomic profiling of three Crataegus species (hawthorn) and their in vitro biological activities

Oxidative stability, structural, and textural properties of margarine enriched with Moringa oleifera leaves extract

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