Impact of circulating miRNA-373 on breast cancer diagnosis through targeting VEGF and cyclin D1 genes

Abstract

Background Breast cancer (BC) is the common primary tumor among females. Hence, there is an urgent need to improve the early prediction and diagnosis of BC. For that reason, the object of the current study is to analyze the expression levels of miRNA-373 and its target genes including vascular endothelial growth factor (VEGF) and cyclin D1 in women with BC. Results Upregulation of miRNA-373 and its target genes was observed in BC patients followed by patients with benign breast lesions compared to downregulation in controls. There was a significant association between the expression level of miRNA-373 and all clinical features. The same associations were observed between its target genes and all clinico-pathological features except hormonal status. The correlation between miRNA-373 and both genes was significant. Conclusions Our results prove that miRNA-373, as an oncomir, would be a vital biomarker for BC diagnosis and prognosis by targeting both VEGF and cyclin D1.

Cancer Biomarkers in Liquid Biopsy for Early Detection of Breast Cancer: A Systematic Review

“…In total, 16 142 articles were identified in the aforementioned databases (Table 1). However, 234 articles were retained for full reading, following the elimination of duplicates and articles whose title and abstract did not meet the eligibility criteria; of these, only 136 12…”

“…As shown in Table 6, highlighted in gray, 28 biomarkers presenting a Sensitivity greater than 90% were identified: miR-17-5p, miR-155, miR-222, 89 miR-202, 60 PTEN, SMAD4, 24 APC, RARB2, 115 miRNA-222, miRNA-373, 92 miR-27a, 85 cfDNA methylation score, 25 HER2 mRNA, 95 miR-21, 28 polymorphism -31G/C in survivin promoter gene, 79 hsa-miR25-3p, hsa-miR-548ar-5p, 80 miR-598-3p, miR-1246, 105 miR-495, 117 telomeric sequences in cfDNA, 121 miR-30c, miR-148a, 39 miR-185-3p, 49 miR-34a, 17 miR10b, miR21, 12 and miRNA-373. 34…”

Cell cycle associated miRNAs as target and therapeutics in lung cancer treatment

“…However, overexpression and accumulation of Cyclin D1 may function as oncogenic protein in several cancer cells and induce genetic changes in other regulatory proteins of cell cycle [ 66 ]. The expression of Cyclin D1 is reduced by the silencing effect of miR-20b and miRNA-373 [ 67 , 68 ].…”

Addressing the Clinical Feasibility of Adopting Circulating miRNA for Breast Cancer Detection, Monitoring and Management with Artificial Intelligence and Machine Learning Platforms

“…On the contrary, the upregulation of circulating miR-21 correlated tightly to radio resistance among BC patients [ 36 ]. Notably, all the circulating miRNAs that were reported to modulate treatment responses in BC patients appeared to share a common function in promoting uncontrolled proliferation and apoptosis evasion among the BC cells [ 61 , 62 ]. For example, miR-373 was reported to upregulate the expression of vascular endothelial growth factor (VEGF) in BC cells, which would lead to enhanced proliferation and angiogenesis [ 62 ].…”

“…Evidently, circulating miRNAs have great potential to be employed as minimally invasive biomarkers in diagnosing BC at an early stage and complementary to the distinguishment of BC based on its clinical and histopathological grading [ 62 , 67 ]. In addition, circulating miRNAs are also helpful to predict the likelihood of relapse, recurrence and treatment responses among BC patients and this is particularly useful in guiding clinicians in planning a personalized treatment approach for different BC patients [ 30 , 68 ].…”