Impact of chlorpyrifos on behavior and histopathological indices in different tissues of freshwater fish Channa punctatus (Bloch)

Stalin, A.; Palani, Suganthi; Mathivani, S; Paray, Bilal Ahmad; Khalid, Muhammad; Gokula, V.; Musthafa, Mohamed Saiyad

doi:10.1007/s11356-019-05165-3

Cited by 44 publications

(11 citation statements)

References 17 publications

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“…The villi fusion in the intestinal mucosa can interfere with physiological function, such as food absorption in fish, which causes the growth and development of fish to be impeded (Kumari and Ahirrao, 2018). The result is in line with the report of Stalin et al (2019) on intestinal damage in villi fusion in freshwater fish Channa punctatus, which was exposed to chlorpyrifos for 20 days. The intestinal goblet cells number was counted with Periodic Acid Schiff (PAS) staining following Ng et al (2005) (Figure 10).…”

Section: Resultssupporting

confidence: 92%

“…According to Watson et al (2014), exposure to organophosphate pesticides showed physiological changes on Zebrafish and Xenopus embryos, such as a decrease in the number of heartbeats per minute. In addition, Stalin et al (2019) showed that exposure to chlorpyrifos insecticide caused behavioral and histopathological changes in various organs of Channa punctatus.…”

Section: Introductionmentioning

confidence: 99%

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Pengaruh Insektisida Klorpirifos Terhadap Struktur Histologi Intestinum Ikan Wader Pari (Rasbora lateristriata Bleeker, 1854)

Nita

Retnoaji²

2022

JPSL

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Chlorpyrifos is one of commonly used insecticides. However, the use of insecticides in agriculture has negative impacts including lethal effect to non-target organisms. Wader pari fish is a type of freshwater fish that lives in rice fields or rivers and can be used as an indicator of water pollution. The purpose of this study was to determine the intestinal histological structure, the histopathological effect of chlorpyrifos on intestine, and population number of goblet cells, respectively. The treatment concentration of 0.001; 0.005; and 0.01 ppm were determined by preliminary trial and exposed for 168 hours. Fish behavior and water quality were monitored. The fish were still alive until the last day of exposure were then made intestinal histological preparations using the paraffin method and staining with Haematoxylin Eosin (HE), Mallory Acid Fuchsin (MAF), Periodic Acid Schiff (PAS). The results showed that the histological structure of the wader pari intestine consisted of mucosal tunica, submucosal tunica, muscularis tunica, and serous tunica. Furthermore, chlorpyrifos has a significant effect on population number of goblet cells. The intestine damages were edema, vacuolization, cloudy swelling, hemorrhage, lysis, and fusion of vili. It could be concluded that chlorpyrifos caused intestinal damage in wader pari fish.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Pengaruh Insektisida Klorpirifos Terhadap Struktur Histologi Intestinum Ikan Wader Pari (Rasbora lateristriata Bleeker, 1854)

Nita

Retnoaji²

2022

JPSL

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“…Abnormal swimming pattern has been detected in Indian major carp, Catla catla [ 45 ], Oryzias latipes [ 46 ], and Cyprinus carpio [ 47 ]. Previously, disorders of convulsions were detected in Channa punctatus reared in water at high level of salinity [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Nuclear and Morphological Alterations in Erythrocytes, Antioxidant Enzymes, and Genetic Disparities Induced by Brackish Water in Mrigal Carp (Cirrhinus mrigala)

Raza

Ghaffar

Hussain

et al. 2022

Oxidative Medicine and Cellular Longevity

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Salinization of aquatic ecosystem, abrupt climate change, and anthropogenic activities cause adverse impact on agricultural land/soil as well as the aquaculture industry. This experimental study was designed to evaluate different biomarkers of oxidative stress, antioxidant enzymes, and genotoxic potential of diverse salinities of brackish water on freshwater fish. A total of 84 fresh water mrigal carp (Cirrhinus mrigala) were randomly segregated and maintained in four groups (T0, T1, T2, and T3) in a glass aquarium under similar laboratory conditions at various salinity levels (0, 3, 5, and 7 parts per thousand) to determine the pathological influence of brackish water. All the fish in groups T1, T2, and T3 were exposed to various salinity levels of brackish water for a period of 90 days while the fish of group T0 served as the control group. The experimental fish reared in different groups T1, T2, and T3 displayed various physical and behavioral ailments. The results revealed significantly augmented quantity of different oxidative stress indicators including reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS) in different visceral tissues (kidneys, liver, and gills) of exposed fish. Different antioxidant enzymes such as reduced glutathione (GSH), peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) along with total proteins were remarkably reduced in the kidneys, gills, and liver tissues. Results showed significantly increased values of different nuclear abnormalities (erythrocyte with micronucleus, erythrocyte with condensed nucleus, and erythrocyte with lobed nucleus) and morphological changes (pear shaped erythrocyte, spindle-shaped erythrocytes, and spherocyte) in red blood cells of experimental fish. The results on genotoxic effects exhibited significantly increased DNA damage in isolated cells of liver, kidneys, and gills of exposed fish. The findings of our experimental research suggested that brackish water causes adverse toxicological impacts on different visceral tissues of fresh water fish at higher salinity level through disruption and disorder of physiological and biochemical markers.

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“…Histopathological alterations/lesions in kidney tissues were determined semi‐quantitatively according to Mishra and Mohanty (2008) and Stalin et al (2019). The histopathological lesions were categorized as 0–5% (no abnormalities), 5–20% (mild), 20–50% (moderate), 50–70% (severe), and >70% extreme severe abnormalities.…”

Section: Methodsmentioning

confidence: 99%

Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation

Lacy

Rahman

2022

J of Applied Toxicology

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One of many noteworthy consequences of increasing societal reliance on pesticides is their predominance in aquatic environments. These pernicious chemicals interact with high temperatures from global climate change, heat waves, and natural variations to create unstable environments that negatively impact organisms' health.To understand these conditions, we examined the dose-dependent effects of environmentally relevant pesticide mixtures (metolachlor, linuron, isoproturon, tebuconazole, aclonifen, atrazine, pendimethalin, and azinphos-methyl) combined with elevated temperatures (22 control vs. 32 C for 4-week exposure) on renin, dinitrophenyl protein (DNP, an indicator of reactive oxygen species, ROS), 3-nitrotyrosine protein (NTP, an indicator of reactive nitrogen species, RNS), superoxidase dismutase (SOD, an antioxidant), and catalase (CAT, an antioxidant) expressions in the kidneys of goldfish (Carassius auratus). Histopathological analysis showed widespread damage to kidney tissues in high temperature and pesticide co-exposure groups, including rupture of the epithelial layer, hemorrhaging, and degeneration of tubular epithelium. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical analyses demonstrated significant declines in renin receptorlike mRNA and protein expressions in kidney tissues under combined exposure to high temperature and pesticides compared with controls; conversely, expression of DNP, NTP, SOD, and CAT increased in kidney tissues under the same conditions. Apoptotic cells were also increased in co-exposure groups as assessed by in situ terminal deoxynucleotidyl transferase dUTP nick labeling (TUNEL) assay. The enhanced apoptosis in kidneys of heat and pesticides co-exposed fish was associated with increased caspase-3 (a protease enzyme) mRNA levels. Our results demonstrated that high temperature and pesticides induced oxidative/nitrative stress (i.e., ROS/RNS), damaged tissues, increased cellular apoptosis, and suppressed renin expression in kidneys of goldfish.

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Impact of chlorpyrifos on behavior and histopathological indices in different tissues of freshwater fish Channa punctatus (Bloch)

Cited by 44 publications

References 17 publications

Pengaruh Insektisida Klorpirifos Terhadap Struktur Histologi Intestinum Ikan Wader Pari (Rasbora lateristriata Bleeker, 1854)

Pengaruh Insektisida Klorpirifos Terhadap Struktur Histologi Intestinum Ikan Wader Pari (Rasbora lateristriata Bleeker, 1854)

Nuclear and Morphological Alterations in Erythrocytes, Antioxidant Enzymes, and Genetic Disparities Induced by Brackish Water in Mrigal Carp (Cirrhinus mrigala)

Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation

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