Impact of Chamomile on Submandibular Salivary Gland of 5-Fluorouracil Treated Rabbits (Histological and Immunohistochemical Study)

Zahawi, Shukria Muhammed

doi:10.4172/2155-9899.1000366

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…An earlier study that showed DM is linked to increased expression of TNF, which causes inflammation and impaired parotid function, validated the findings of this study (Li et al, 2019). In addition, a previous study (Zahawi, 2015) claimed that such inflammatory responses might be intended to increase blood flow to damaged tissue. Insulin was able to ameliorate the histological appearance of the parotid gland in the insulincontrolled diabetic parotid glands, with some pyknotic nuclei remaining and some architectural changes being made.…”

Section: Discussionsupporting

confidence: 85%

Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1

Morsy

Abdelwahab

Ibrahim

et al. 2023

Egyptian Society of Clinical Toxicology Journal

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Background: Hyperglycemia associated with the diabetes mellitus (DM) commonly results in structural abnormalities and hyposalivation in the parotid glands. An antioxidant action in a potent multivitamin called Antox (ANX) aids in reducing oxidative stress. Aim of study: The current study examined the role of ANX in preventing complications from diabetes in the parotid gland, as well as its potential mechanisms. Material and methods: 36 rats were randomly divided into six groups; each group contained six rats. Group 1 (Negative (-ve) control): the rats received only regular food and water. Group 2 (vehicle group): rats received a single i.p. dose of citrate buffer as a vehicle. Group 3: rats were given ANX by oral gavage with a polyethylene canula 0.5 mm in a volume not to exceed 0.3 ml/100 gm at a dose of 10 mg/kg/day. Group 4 (Diabetic/ DM): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer. Group 5 (DM+ Insulin): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer and insulin (Mixtard 30/70; Novo Nordisk) 1 U/100 gm once daily subcutaneous (S.C.). Group 6 (DM+ Insulin + ANX): rats received a single i.p. dose of 50 mg/kg of STZ and insulin 1 U/100 gm /day/S.C. then received ANX orally in a dose of 10 mg/kg/day. All medications were given for 4 weeks. Rats were anaesthetized, and the parotid tissues were obtained for biochemical analysis to measure Malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and nitric oxide (NO), for Histopathological examination (Hematoxylin and Eosin staining, Masson trichrome stain) and immunohistochemistry using (8-OHdG, α-SMA and BAX). Results: Remarkably, co-administration of ANX with insulin significantly reduced malondialdehyde (MDA) levels and increased expression of 8-hydroxy-2'-deoxyguanosine (8-OHdG) while enhancing the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant capacity (TAC). Additionally, compared to the diabetic rat group and the diabetic group receiving insulin, the combination of ANX and insulin resulted in a considerably decreased expression of Bcl-2, Associated X-protein (Bax) and Smooth muscle alpha-actin (α-SMA), with significant reduction in interleukin-1beta (IL1β) level in relation to diabetic group. Conclusion: In comparison to insulin administration alone, co-delivery of ANX with insulin throughout diabetes treatment was more effective in preserving the structure and function of the parotid gland.

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Section: Discussionsupporting

confidence: 85%

Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1

Morsy

Abdelwahab

Ibrahim

et al. 2023

Egyptian Society of Clinical Toxicology Journal

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“…The determined marked dilatation and congestion of the blood vessels were equally reported following MTX and 5-flourouracil [19,43] . These inflammatory reactions could aim to transport more blood to the degenerated tissues [44] .…”

Section: Discussionmentioning

confidence: 89%

Therapeutic mechanisms of granulocyte-colony stimulating factor in methotrexate-induced parotid lesion in adult rats and possible role of telocytes: A histological study

Omar

Yousry

Farag

2018

Egyptian Journal of Histology

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Background: Worldwide, Methotrexate (MTX) is used to treat neoplastic and non-neoplastic diseases despite its severe side-effects. One of these adverse effects is salivary dysfunction that can aggravate MTX mucotoxic effects. Such effects interfere with the patients' quality of life and increase morbidities and mortalities. Available treatments for salivary dysfunction have many drawbacks. Mobilization of bone marrow haematopoietic stem cells (BMHSCs) by human Granulocyte-Colony Stimulating Factor (G-CSF) used in many diseases showed promising results. Aim of the work: To detect the therapeutic effect of G-CSF on MTX-induced parotid lesion highlighting its possible mechanisms and the role of telocytes. Materials and Methods: Forty-one albino rats were divided into 3 groups; control, MTX and Neupogen-treated groups. All animals were weighed and sacrificed after 6 days. Parotid homogenates of 6 rats from each group were used to measure total protein concentration, amylase activity and malondialdehyde (MDA) value. The right parotids of the remaining animals of each group were processed to Paraffin blocks while, the left ones were processed to resin blocks. Paraffin sections stained with Hematoxylin and Eosin and immunohistochemical stains for CD34 and platelet-derived growth factor receptor-alpha (PDGFR-α) as well as resin semithin and ultrathin sections were examined. Body weight, biochemical results, number of immune-positive cells, difference between numbers of CD34+ and PDGFR-α+ cells (stem cells number), acini and ducts numbers and diameters were statistically analysed. Results: MTX group revealed diarrhoea, decreased body weight in addition to, degenerated acinar and ductal cells, and telocytes. However, Neupogen-treated group demonstrated almost normal histological features. Immunohistochemically, the treated group showed significant increase in CD34+ cells, PDGFR-α + cells and stem cells number versus MTX group. Conclusions: G-CSF had a therapeutic effect on MTX-induced parotid degeneration through its direct effects, BMHSCs mobilization and telocytes preservation.

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“…Cure et al [ 44 ] reported that MTX treatments resulted in enhanced subepithelial vascularity. Similarly, Zahawi [ 45 ] reported that in the submandibular salivary gland of rabbits, after chemotherapy, marked dilatation, and congestion of the blood vessels appeared in the MTX group. According to Omar et al [ 41 ], this dilatation and congestion may be attributable to the inflammatory reaction associated with MTX treatment, which increases transendothelial permeability.…”

Section: Discussionmentioning

confidence: 78%

The Prospective Effect of Green Tea versus Pomegranate Peels Extracts on Submandibular Salivary Glands of Albino Rats after Methotrexate Administration (Histological and Immunohistochemical Study)

Taha,

Abdel-Latif,

Said

2024

International Journal of Dentistry

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Background. There is curiosity in the use of substances that can stop cell damage. Antioxidants are substances that can prevent free radicals from damaging cells, and they can be used to treat and avoid a wide variety of illnesses. Objectives. The current investigation set out to evaluate the histological changes brought on by a single high dose of methotrexate in the submandibular glands of rats treated with green tea and pomegranate peel extract, both are well-known as antioxidants. Materials and Methods. Forty-eight healthy Albino rats were used in the current study. Animals were divided into six groups. Group 1: Vehicle group which is control group, Group 2: methotrexate treated group, Group 3: green tea control group, Group 4: pomegranate peel extract control group, Group 5: green tea + methotrexate group, and Group 6: pomegranate peel extract + methotrexate group. Rats of all groups were left 1 week after the end of the treatment. Cervical dislocation was used to kill all of the rats. Samples were gained from the rats’ submandibular salivary glands of different groups for histological and immunohistochemical evaluation. Results. Green tea + methotrexate group showed improvement in the histological picture of the submandibular salivary gland compared to methotrexate group and the pomegranate peel extract + methotrexate group. Conclusion. The antioxidant activity of green tea is more potent than that of pomegranate peels extract in blocking methotrexate that induced cytotoxicity in the submandibular salivary glands of rats. As a result, it can be administered to people undergoing cancer treatment as a safeguard for their salivary glands.

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Impact of Chamomile on Submandibular Salivary Gland of 5-Fluorouracil Treated Rabbits (Histological and Immunohistochemical Study)

Cited by 3 publications

References 23 publications

Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1

Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1

Therapeutic mechanisms of granulocyte-colony stimulating factor in methotrexate-induced parotid lesion in adult rats and possible role of telocytes: A histological study

The Prospective Effect of Green Tea versus Pomegranate Peels Extracts on Submandibular Salivary Glands of Albino Rats after Methotrexate Administration (Histological and Immunohistochemical Study)

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