Background: To our knowledge, no large studies reporting bone marrow (BM) derived cell counts of children and adults with non-malignant diseases have been published so far. Thus, the primary objective was to evaluate BM-derived CD34+ and leukocyte cell counts in 729 female and male patients of different age groups who underwent autologous cell-based therapy for non-malignant diseases. Methods: For this study, a retrospective data analysis of laboratory parameters including BM-aspirated and post-centrifuge concentrated CD34+ cells and leukocytes was performed. Associations and differences of cell counts between age groups, gender, and diagnose related group were evaluated.Results: Included were data of 187 female and 542 male patients aged between 2 and 75 years, who underwent autologous cell-based therapy for various non-malignant diseases. The median percentage of CD34+ cells of leukocytes was 1.10% in BM-aspirate (BMA) and 0.96% in post-centrifuge BMA concentrate (BMAC). Significant moderate positive correlations were observed between CD34+ cells (count/µl) and leukocytes (count/µl) in BMA and in BMAC. Significant strong negative correlations were detected between age (years) and CD34+ cells (count/µl/kg) in BMA and in BMAC. No significant differences regarding CD34+ cells (count/µl) in BMA were detected between adults, while significant differences regarding cell counts were detected between diagnose related groups, but not between females and males.Conclusions: This study demonstrated BM-derived CD34+ and leukocyte cell counts in BMA and BMAC of 729 patients with various non-malignant diseases. While BM-derived CD34+ cells were significantly higher in younger patients, similar cell counts were detected within adults.