Impact of CCR2 and SDF1 Polymorphisms on Disease Progression in HIV‐Infected Subjects in Thailand

Ammaranond, Palanee; Sanguansitthianan, Sayompoo; Phaengchomduan, Poonlaph; Sae-Lee, Chanachai; Mardkhumchan, Sirimarn

doi:10.1002/jcla.21559

Cited by 5 publications

(6 citation statements)

References 39 publications

(79 reference statements)

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“…CCR2-V64I (rs1799864) polymorphism, in which adenine is substituted for guanine at CCR2 position 190, changes the amino acid valine (V) to isoleucine (I) at position 64 within the first transmembrane domain of the protein (Rabkin et al, 1999). Although CCR2-V64I polymorphism does not influence HIV-1 transmissibility, studies have shown that patients carrying the mutant 64I allele have better prognoses when infected with this virus (Mahajan et al, 2010;Ammaranond et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of CCR5-Δ32 and CCR2-V64I polymorphisms in a mixed population from northeastern Brazil

Ferreira-Fernandes

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Chemokines are low-molecular weight proteins that play a key role in inflammatory processes. Genomic variations in chemokine receptors are associated with the susceptibility to various diseases. Polymorphisms in chemokine receptor type 5 (CCR5)-D32 and CCR2-V64I are related to human immunodeficiency virus infection resistance, which has led to genetic association studies for several other diseases. Given the heterogeneous distribution of these polymorphisms in different global populations and within Brazilian populations, we analyzed the prevalence of CCR5-D32 and CCR2-V64I polymorphisms in a mixed population from northeastern Brazil. The study included 223 individuals from the general population of the city of Parnaíba, Piauí, who had a mean age of 73 years. Of these individuals, 37.2% were men 11711 CCR5-D32 and CCR2-V64I polymorphisms in Piauí, Brazil ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 11710-11718 (2015) and 62.8% were women. Polymorphisms were analyzed using DNA extracted from peripheral blood leukocytes by using polymerase chain reaction alone (CCR5-D32) or accompanied by restriction endonuclease digestion (CCR2-V64I). In both cases, the genotypes were determined using 8% polyacrylamide gel electrophoresis and silver nitrate staining. The population conformed to Hardy-Weinberg equilibrium for both the loci studied. No individuals were homozygous for allele-D32, which was present in 1.8% of the population, whereas allele-64I was present in 13.9% of the participants studied; 74.9% were homozygous for the wild-type allele, while 22.4 and 2.7% were heterozygous and homozygous for the mutant allele, respectively. Additional studies are needed to investigate the relationship between these polymorphisms and disease etiopathogenesis in reference populations.

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Section: Introductionmentioning

confidence: 99%

Prevalence of CCR5-Δ32 and CCR2-V64I polymorphisms in a mixed population from northeastern Brazil

Ferreira-Fernandes

et al. 2015

Genet. Mol. Res.

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“…That study found the effect of CCR5 genotypes on HIV disease course had diminished after controlling for other factors, including initial CD4 count, initial viral load and the virus phenotype factors (29). In addition, previous studies on the influence of CCR5-∆32 on CD4 recovery during HAART have shown some contradiction with positive (25) and negative findings obtained (29,30). These conflicting findings could be related to the different geographical location or study population, for example Caucasians versus Thais population (30).…”

Section: Discussionmentioning

confidence: 88%

CCR5 Polymorphisms and its Relationship with HIV Susceptibility, Viral Load and CD4 Count in Early Antiretroviral Therapy among HIV Patients in Selangor and Terengganu

Mohamad Isa,

Abu Bakar,

Zolraimi

et al. 2023

MJMHS

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Introduction: Early studies have suggested the role of C-C chemokine receptor type 5 (CCR5) polymorphisms in influencing HIV pathogenesis and phenotypes, including the protection against HIV infection and delaying disease progression to AIDS. This study aimed to further determine the impact of CCR5 variants (CCR5-Δ32 and CCR5-R223Q) on HIV susceptibility, viral load suppression and CD4 recovery during highly active antiretroviral therapy (HAART) among Malaysian HIV patients. Methods: This cross-sectional study involved 182 HIV-infected who were recruited from three out-patient clinics, and 150 non-HIV subjects from Malay, Chinese and Indian ethnicities. CD4 count and viral load data at 4-6 months (t1) and 8-12 months (t2) after starting HAART were gathered from hospital records. Chi-square test was used to analyse the correlation between CCR5 variants with dependent variables. Results: Heterozygous CCR5-Δ32 and CCR5-R223Q occurred in a percentage of 0.5% (1/182) and 1.7% (3/182) among HIV patients respectively, while none of homozygous mutant for CCR5-Δ32 and CCR5-R223Q were found. CCR5-R223Q was found more frequently in non-HIV as compared to the HIV group (P=0.018). However, both polymorphisms were not found to be correlated with CD4 recovery to ≥500 cells/mm3 (P>0.05) and viral load suppression ≤50 copies/mL (P>0.05). Conclusion: CCR5-R223Q and CCR5-Δ32 alleles probably have no modifying effects on HIV susceptibility virological and immunological recoveries in the first 12 months of HAART, partially due to the low prevalence of these mutations in the studied population.

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“…14,16 In this study, frequency of the A allele of CXCL12 G801A polymorphism was observed as 0.256 while previous studies in Thai HIV-seropositive patients and blood donors reported the frequencies ranging from 0.199-0.43. 20,21 The CXCL12 G801A genotypes were in Hardy-Weinberg equilibrium (p > 0.05), indicating that genetic background of the study population remains constant. Our data indicated significant associations of the CXCL12 G801A SNP with transaminitis and significant hepatic fibrosis assessed by APRI > 0.5.…”

Section: Discussionmentioning

confidence: 95%

“…6,32 Our analysis indicated a high prevalence of HIV patients with chronic hepatic injury, which possibly develop to more severe fibrosis and cirrhosis as observed in the studies in HIV patients who were coinfected with HCV and on ART for longer duration. 32,33 CXCL12 G801A polymorphism has been well characterized in different ethnic groups and established to have an impact on susceptibility to HIV infection, HIV disease progression and ART response 11,13,[19][20][21][22] whereas few studies indicate its role in liver disease caused by hepatitis viruses and hepatocellular carcinoma. 14,16 In this study, frequency of the A allele of CXCL12 G801A polymorphism was observed as 0.256 while previous studies in Thai HIV-seropositive patients and blood donors reported the frequencies ranging from 0.199-0.43.…”

Section: Discussionmentioning

confidence: 99%

“…17,18 CXCL12 is also well known to contribute dramatically to pathogenesis and progression of HIV infection and many previous studies have indicated an impact of CXCL12 gene polymorphisms including the G801A SNP on resistance of HIV infection, progression to AIDs and responses to ART. 11,13,[19][20][21][22] However, influence of the CXCL12 gene polymorphism on chronic liver diseases developed in HIV-infected patients is still unknown.…”

Section: Evaluation Of Liver Complicationmentioning

confidence: 99%

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CXCL12 G801A polymorphism is associated with significant liver fibrosis in HIV-infected Thais: a cross-sectional study

Chiraunyanann¹,

Changsri²,

Sretapunya

et al. 2018

Asian Pac J Allergy Immunol

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Background: Previous studies indicate high prevalence of liver diseases in HIV-infected patients, and their genetic risk factors are still unclear. The chemokine CXCL12 plays important roles in development of chronic liver injury and a single nucleotide polymorphism (SNP) G to A change at position 801 in CXCL12 gene has been demonstrated to affect CXCL12 production levels.Objective: This study aimed to analyze the association of CXCL12 G801A SNP with liver complication in HIV-infected Thais. Methods:A cross-sectional study was conducted in 164 patients who were evaluated for transaminitis and significant liver fibrosis, defined by fibrosis-4 (FIB-4) score and AST to platelet ratio index (APRI), and genotyped for the SNP using tetra-primer PCR-SSP.Results: There were high rates of patients with transaminits (28.0%), and significant liver fibrosis by FIB-4 score (18.9%) and by APRI (14.0%). The CXCL12 G801A AA/GA genotypes were significantly associated with transaminitis (p = 0.014) and significant fibrosis by APRI (p = 0.020). Univariate and multivariate analyses identified the AA/GA genotypes as predictive factors for significant fibrosis (OR 6.8, 95%CI 1.7-28.2, p = 0.008), together with age older than 40 years, CD4 + cell count < 350 cells/µl and hepatitis B and/or C virus coinfection. The significantly higher medians of APRI and FIB-4 score, in patients with AA/GA than those with GG genotypes (p < 0.05) were observed in the ART-naïve, but not ART-experienced groups. Conclusion:The CXCL12 G801A AA/GA genotypes are significant predictive factors for hepatic fibrosis potentially in the ART-naïve HIV-infected Thais.

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Impact of CCR2 and SDF1 Polymorphisms on Disease Progression in HIV‐Infected Subjects in Thailand

Abstract: The mutation of CCR2 and SDF1 genes showed a significant difference in the distribution of CD4+ T-cell numbers (P < 0.001) whereas mutation of chemokine coreceptor CCR5 was not appeared to be associated with the impact of CD4+ T-cell counts.

Cited by 5 publications

References 39 publications

Prevalence of CCR5-Δ32 and CCR2-V64I polymorphisms in a mixed population from northeastern Brazil

Prevalence of CCR5-Δ32 and CCR2-V64I polymorphisms in a mixed population from northeastern Brazil

CCR5 Polymorphisms and its Relationship with HIV Susceptibility, Viral Load and CD4 Count in Early Antiretroviral Therapy among HIV Patients in Selangor and Terengganu

CXCL12 G801A polymorphism is associated with significant liver fibrosis in HIV-infected Thais: a cross-sectional study

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