Impact of CCR1 silencing on the assembly of lignified secondary walls in <i>Arabidopsis thaliana</i>

Ruel, K.; Berrio-Sierra, Jimmy; Mir-Derikvand, Mohammad; Pollet, Brigitte; Thévenin, Johanne; Lapierre, Catherine; Jouanin, Lise; Joseleau, J.P.

doi:10.1111/j.1469-8137.2009.02951.x

Cited by 93 publications

(96 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As indicated by Bjurhager et al (2010), H 2 O 2 removed about 30% of lignin from Populus wood which affect 10% reduction of wood density. The similar trend also observed in both tobacco and Arabidopsis taliana in which the reduction of lignin from the wood had an effect on the ultrastructural architecture of the wood fibre wall (Ruel et al 2009). …”

Section: Effects Of Bleaching On Physical Properties Of Rubberwoodsupporting

confidence: 52%

Enhancement of Hevea brasiliensis properties through chemical application

Kadir

Jantan

2016

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The effects of four different types of bleaching agents (hydrogen peroxide, sodium hypochlorite, sodium chlorite and oxalic acid) mixed together with 2% boron-based preservative (Celbor SP) were studied on green sawn rubberwood. Two concentration levels (1% and 2%) of were used. Whitish values (W) and colour changes (ΔE) of sawn rubberwood were assessed before and after chemical treatment using a colour meter. Preliminary observation indicated that timber treated with 1% and 2% hydrogen peroxide produced better and more homogeneous colour properties. Hydrogen peroxide treated timber gave higher whitish values (6.23 and 9.91 for 1% and 2% solution, respectively) and lower colour changes (8.49 and 5.51 for 1% and 2% solution, respectively) when compared to the other three chemicals. Evaluation on the effects of bleaching (hydrogen peroxide with a higher level of concentration) on physical, mechanical and biological properties of rubberwood also have been determined in this study.

show abstract

Section: Effects Of Bleaching On Physical Properties Of Rubberwoodsupporting

confidence: 52%

Enhancement of Hevea brasiliensis properties through chemical application

Kadir

Jantan

2016

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…This contrasts with previous studies, in which changes in the secondary wall, particularly the S2 layer (e.g. concentric loosening of S2 wall layer), has been reported in poplar (Leple et al 2007), Arabidopsis (Ruel et al 2009), and flax (Day et al 2009) in knock-down mutants affecting enzymes of lignin biosynthesis. The TEM observations were however consistent with the histochemical and UV-observations of fibers and vessel wall architecture, suggesting that any changes were likely to be on a supermolecular rather than ultrastructural level.…”

Section: Ultrastructural Tem Observationscontrasting

confidence: 53%

Anatomical and Immunocoverage Observations on SuSy, C4H, and Pectate Lyase Family Protein Down-regulated Aspens Genotypes

Sandquist¹,

Norell²,

Takabe³

et al. 2015

BioResources

View full text Add to dashboard Cite

This study aims to complement previous studies on down-regulation of sucrose synthase and cinnamate 4-hydroxylase in hybrid aspen (Bjurhager et al. 2010;Gerber et al. 2014) with a quantitative anatomical comparison (Sandquist et al. 2015). The main focus was placed on evaluating quantitative modifications to fiber morphology and lignin composition. This was achieved by combining results from light-, electron-, and UV-microscopy together with histochemical staining. Overall there was good agreement between the morphological results in this study compared to the previously published chemical and physical reports, particularly on cinnamate 4-hydroxylase down-regulated aspen. The previously reported statistical correlations between fiber lumen area and plant diameter, and fiber length and tree height (Sandquist et al. 2015) have been further refined. Knock-down of genes coding for biologically important proteins such as sucrose synthase and cinnamate 4-hydroxylase had broad spanning morphological impacts. Downregulated sucrose synthase genotype showed significantly reduced fiber development, possibly with altered S/G ratio. Down-regulated cinnamate 4-hydroxylase genotype showed an overall reduction of lignin in fibers as indicated from both chemical staining and UV-microscopy measurements. Down-regulated pectate lyase genotype showed no overall significant effects on fiber morphology, ultrastructural, nor lignin staining.

show abstract

“…It is known from experiments with dehydrogenation polymers that the conditions during polymerization have a large im-pact on lignin structure. Similarly, in planta, the structures of the polymers also vary among cell types and individual cell wall layers (Ruel et al, 2009). Furthermore, the sequencing method allows oligolignols that do not fit with our current knowledge of lignin structure to be identified, thus pinpointing those that deserve further research to characterize novel units and bonds.…”

Section: Discussionmentioning

confidence: 99%

Mass Spectrometry-Based Sequencing of Lignin Oligomers

Morreel

Dima²,

Kim³

et al. 2010

Plant Physiol.

168

213

View full text Add to dashboard Cite

Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for the second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from the combinatorial radical-radical coupling of monolignols and many other less abundant monomers. This polymerization process leads to a plethora of units and linkage types that affect the physicochemical characteristics of the cell wall. Current methods to analyze the lignin structure focus only on the frequency of the major monomeric units and interunit linkage types but do not provide information on the presence of less abundant unknown units and linkage types, nor on how linkages affect the formation of neighboring linkages. Such information can only be obtained using a sequencing approach. Here, we describe, to our knowledge for the first time, a sequencing strategy for lignin oligomers using mass spectrometry. This strategy was then evaluated on the oligomers extracted from wild-type poplar (Populus tremula 3 Populus tremuloides) xylem. In total, 134 lignin trimers to hexamers were observed, of which 36 could be completely sequenced. Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit.Lignin is an aromatic heteropolymer that is mainly present in secondary-thickened plant cell walls, allowing the transport of water and nutrients and providing the necessary strength for the plant to grow upwardly Vanholme et al., 2008Vanholme et al., , 2010. In angiosperms, lignin is predominantly composed of guaiacyl (G) and syringyl (S) units that are derived from combinatorial radical-radical coupling of the monolignols coniferyl and sinapyl alcohol, respectively Fig. 1A). Following oxidation of the monolignols by peroxidase and/or laccase, the resulting electron-delocalized radical has unpaired electron density at its 1-, 3-, O-4-, 5-, and 8-positions (Fig. 1B); note that much of the lignin literature uses Greek letters for the side chain, a, b, and g for the 7-, 8-, and 9-positions. As radical coupling at the 8-position is favored, coupling with another monolignol radical affords, after rearomatization, a mixture of dehydrodimers with 8-8-, 8-5-, and 8-O-4-linkages (Fig. 1C). Following dimerization, polymerization will continue by the coupling of the 8-position of an incoming monolignol radical to the O-4-position of the dimer's phenolic end. In the case of a G dimer, coupling can also occur, albeit at a lower frequency, to the 5-position. Thus, chain elongation creates 8-5-and 8-O-4-linkages (Adler, 1977). Besides the monolignols and other monomers that are present in minor amounts , the plasticity of lignin polymerization permits the incorporation of any phenolic that enters the lignification site, subject to its chemical cross-coupling propensit...

show abstract

Impact of CCR1 silencing on the assembly of lignified secondary walls in Arabidopsis thaliana

Cited by 93 publications

References 61 publications

Enhancement of Hevea brasiliensis properties through chemical application

Enhancement of Hevea brasiliensis properties through chemical application

Anatomical and Immunocoverage Observations on SuSy, C4H, and Pectate Lyase Family Protein Down-regulated Aspens Genotypes

Mass Spectrometry-Based Sequencing of Lignin Oligomers

Contact Info

Product

Resources

About