Impact of Carbon Source Supplementations on <i>Pseudomonas aeruginosa</i> Physiology

Sauvage, Salomé; Gaviard, Charlotte; Tahrioui, Ali; Coquet, Laurent; Le, Hung; Alexandre, Stéphane; Araar, Abdelkrim; Bouffartigues, Emeline; Lesouhaitier, Olivier; Chevalier, Sylvie; Jouenne, Thierry; Hardouin, Julie

doi:10.1021/acs.jproteome.1c00936

Cited by 8 publications

(5 citation statements)

References 72 publications

(129 reference statements)

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“…This can in turn have a profound impact on their growth rate, virulence, phenotypes, and resistance to antibiotics [9,[67][68][69][70]. Earlier studies have described the role of carbon sources in the metabolism of P. aeruginosa [71][72][73][74]. Our study highlights the role of malonate as a sole carbon source in inducing a global stress response in P. aeruginosa.…”

Section: Discussionmentioning

confidence: 77%

Global stress response inPseudomonas aeruginosaupon malonate utilization

Bisht,

Elmassry,

Al Mahmud

et al. 2024

Preprint

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Versatility in carbon source utilization assistsPseudomonas aeruginosain its adaptation to various niches. Recently, we characterized the role of malonate, an understudied carbon source, in quorum sensing regulation, antibiotic resistance, and virulence factor production inP. aeruginosa. These results indicate that global responses to malonate metabolism remain to be uncovered. We leveraged a publicly available metabolomic dataset on human airway and found malonate to be as abundant as glycerol, a common airway metabolite and carbon source forP. aeruginosa. Here, we explored and compared adaptations ofP. aeruginosaUCBPP-PA14 (PA14) in response to malonate or glycerol as a sole carbon source using transcriptomics and phenotypic assays. Malonate utilization activated glyoxylate and methylcitrate cycles and induced several stress responses, including oxidative, anaerobic, and metal stress responses associated with increases in intracellular aluminum and strontium. Some induced genes were required for optimal growth ofP. aeruginosain malonate. To assess the conservation of malonate-associated responses amongP. aeruginosastrains, we compared our findings in strain PA14 with other lab strains and cystic fibrosis isolates ofP. aeruginosa. Most strains grew on malonate as a sole carbon source as efficiently as or better than glycerol. While not all responses to malonate were conserved among strains, formation of biomineralized biofilm-like aggregates, increased tolerance to kanamycin, and increased susceptibility to norfloxacin were the most frequently observed phenotypes. Our findings reveal global remodeling ofP. aeruginosagene expression during its growth on malonate as a sole carbon source that is accompanied by several important phenotypic changes. These findings add to accumulating literature highlighting the role of different carbon sources in the physiology ofP. aeruginosaand its niche adaptation.ImportancePseudomonas aeruginosais a notorious pathogen that causes local and systemic infections in immunocompromised individuals. Different carbon sources can uniquely modulate metabolic and virulence pathways inP. aeruginosa, highlighting the importance of the environment that the pathogen occupies. In this work, we used a combination of transcriptomic analysis and phenotypic assays to determine how malonate utilization impactsP. aeruginosa,as recent evidence indicates this carbon source may be relevant to certain niches associated within the human host. We found that malonate utilization can induce global stress responses, alter metabolic circuits, and influence various phenotypes ofP. aeruginosathat could influence host colonization. Investigating the metabolism of malonate provides insight intoP. aeruginosaadaptations to specific niches where this substrate is abundant, and how it can be leveraged in the development of much-needed antimicrobial agents or identification of new therapeutic targets of this difficult-to-eradicate pathogen.

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Section: Discussionmentioning

confidence: 77%

Global stress response inPseudomonas aeruginosaupon malonate utilization

Bisht,

Elmassry,

Al Mahmud

et al. 2024

Preprint

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“…This might be that both media rich in various sugars as a carbon source (Supplementary Table S2) resulted in alteration of bacterial cell wall structure. It is well-known that physiological microorganisms (cell wall structure, virulence, biofilm formation, or antibiotic resistance) are relevant on carbon sources for growth such reports in Pseudomonas aeruginosa PA14 49 and in Candida albicans 50 . The proteomic analysis should be evaluated to describe more details in future.…”

Section: Resultsmentioning

confidence: 99%

Physiological changes in Rhodococcus ruber S103 immobilized on biobooms using low-cost media enhance stress tolerance and crude oil-degrading activity

Naloka

Jaroonrunganan

Woratecha

et al. 2022

Sci Rep

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For economic feasibility, sugarcane molasses (0.5%, w/v) containing K2HPO4 (0.26%, w/v) and mature coconut water, low value byproducts, were used in cultivation of Rhodococcus ruber S103 for inoculum production and immobilization, respectively. Physiological changes of S103 grown in low-cost media, including cell hydrophobicity, saturated/unsaturated ratio of cellular fatty acids and biofilm formation activity, enhanced stress tolerance and crude oil biodegradation in freshwater and even under high salinity (5%, w/v). Biobooms comprised of S103 immobilized on polyurethane foam (PUF) was achieved with high biomass content (1010 colony-forming units g−1 PUF) via a scale-up process in a 5-L modified fluidized-bed bioreactor within 3 days. In a 500-L mesocosm, natural freshwater was spiked with crude oil (72 g or 667 mg g−1 dry biobooms), and a simulated wave was applied. Biobooms could remove 100% of crude oil within only 3 days and simultaneously biodegraded 60% of the adsorbed oil after 7 days when compared to boom control with indigenous bacteria. In addition, biobooms had a long shelf-life (at least 100 days) with high biodegradation activity (85.2 ± 2.3%) after storage in 10% (w/v) skimmed milk at room temperature. This study demonstrates that the low-cost production of biobooms has potential for future commercial bioremediation.

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“…The supernatants collected between 2 h and 12 h and at 24 h and 48 h of incubation, were diluted 2- and 10-fold, respectively. LC–MS/MS analytical conditions were adapted from methods previously described ( Déziel et al, 2000 ; Bazire et al, 2005 ; Sauvage et al, 2022 ). Analysis of biosurfactants was performed with a 1,290 Infinity II UHPLC system coupled to a 6545XT Advance-Bio Q-TOF mass spectrometer (Agilent Technologies, United States).…”

Section: Methodsmentioning

confidence: 99%

BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa

Wallart,

Ben Mlouka,

Saffiedine

et al. 2024

Front. Microbiol.

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Previously, we pointed out in P. aeruginosa PAO1 biofilm cells the accumulation of a hypothetical protein named PA3731 and showed that the deletion of the corresponding gene impacted its biofilm formation capacity. PA3731 belongs to a cluster of 4 genes (pa3732 to pa3729) that we named bac for “Biofilm Associated Cluster.” The present study focuses on the PA14_16140 protein, i.e., the PA3732 (BacA) homolog in the PA14 strain. The role of BacA in rhamnolipid secretion, biofilm formation and virulence, was confirmed by phenotypic experiments with a bacA mutant. Additional investigations allow to advance that the bac system involves in fact 6 genes organized in operon, i.e., bacA to bacF. At a molecular level, quantitative proteomic studies revealed an accumulation of the BAC cognate partners by the bacA sessile mutant, suggesting a negative control of BacA toward the bac operon. Finally, a first crystallographic structure of BacA was obtained revealing a structure homologous to chaperones or/and regulatory proteins.

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Impact of Carbon Source Supplementations on Pseudomonas aeruginosa Physiology

Cited by 8 publications

References 72 publications

Global stress response inPseudomonas aeruginosaupon malonate utilization

Global stress response inPseudomonas aeruginosaupon malonate utilization

Physiological changes in Rhodococcus ruber S103 immobilized on biobooms using low-cost media enhance stress tolerance and crude oil-degrading activity

BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa

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