Impact of carbon and nitrogen nutrition on the quality, yield and composition of blastospores of the bioinsecticidal fungus Paecilomyces fumosoroseus

Abstract

The impact of growing cultures of Paecilomyces fumosoroseus in liquid media containing four combinations of glucose and casamino acids (8 g l(-1) or 80 g l(-1) glucose, 1.32 g l(-1) or 13.2 g l(-1) casamino acids) was evaluated, based on blastospore production, germination rate, viability after freeze-drying and short-term storage stability. When blastospores were produced using a high casamino acid concentration, blastospore yields and germination rates were significantly higher (13.2-18.5 x 10(7) blastospores ml(-1), 50-60% germination after 4 h), compared to cultures grown in media containing lower casamino acid concentrations (0.4-2.3 x 10(7) blastospores ml(-1), 10-20% germination after 4 h). Chemical analyses of blastospore composition showed that accelerated blastospore germination may be related to increased proteinaceous reserves rather than to glycogen or lipid accumulation. Tolerance to freeze-drying by blastospores suspended in spent medium was enhanced by a high initial casamino acid concentration in the culture medium (75% survival) and by the residual glucose concentrations in the spent medium. Under the conditions of this study, the storage stability of blastospores of P. fumosoroseus was unaffected by the nutritional condition in which they were produced.

Cytosolic streaming in vegetative mycelium and aerial structures of Aspergillus niger

“…Previously, it has been shown that the age of the culture as well as environmental conditions affect properties ( e.g. viability and cytotoxicity) of fungal spores (Hallsworth & Magan 1996, Cliquet & Jackson 1999, Murtoniemi et al 2003, Cliquet & Jackson 2005). Normally, spores are collected from the whole mycelium.…”

“…This study indicates that variability in spore properties can be reduced by extracting spores from selected parts of the colony. Defined spore properties are of interest for biocontrol applications (Cliquet & Jackson 1999, Cliquet & Jackson 2005) but may also be of interest for starter cultures of fungal fermentations.…”

Newly Isolated Paecilomyces lilacinus and Paecilomyces javanicus as Novel Biocontrol Agents for Plutella xylostella and Spodoptera litura

“…In general, the success of microbial biopesticides depends on obtaining the pathogen at competitive prices to permit its mass-production and commercialization (Gao et al, 2007). Production processes for fungal biopesticides must be low-cost, and yield high concentrations of viable, virulent, persistent spores that can be stabilized to provide a product shelf-life of 12~18 months (Cliquet and Jackson, 2005).…”

Non-edible Oil Cakes as a Novel Substrate for DPA Production and Augmenting Biocontrol Activity of Paecilomyces variotii

“…Reported by several workers that media constituents simply affect the virulence of biocontrol agents against plant pathogens (Cliquet and Jackson, 2005; Nisha and Kalaiselvi, 2016) and its composition plays a significant role in growing fungal mycelia with increased sporulation (Sun and Liu, 2006; Gao et al, 2007). Liu and Chen (2002) reported that fungi can use wide range of substrates as carbon and nitrogen sources.…”

“…Carbon-Nitrogen ratio as well as type of sugar in media plays an important role in determining the pathogenicity of the fungus (Vidal et al, 1998; Gao et al, 2007; Zhao et al, 2013; Nisha and Kalaiselvi, 2016). Therefore, nitrogen and carbon sources were screened for the production of bioactive molecule because they may influence the activity of phytopathogens, and enhances antibiotics production in biocontrol strains and contribute to the variability of biocontrol in different soils and also on host crops (Milner et al, 1995; Thomashow and Weller, 1996; Cliquet and Jackson, 2005; Moorthi et al, 2015). …”