Impact of bovine serum albumin – A protein corona on toxicity of ZnO NPs in environmental model systems of plant, bacteria, algae and crustaceans

Janani, B.; Raju, Lija L.; Thomas, Ajith M.; Alyemeni, Mohammed Nasser; Dudin, Gani Asa; Wijaya, Leonard; Alsahli, Abdulaziz Abdullah; Ahmad, Parvaiz; Khan, S. Sudheer

doi:10.1016/j.chemosphere.2020.128629

Cited by 30 publications

(9 citation statements)

References 80 publications

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“…Changes in the metabolic activity of six cancer and two non-tumor cell lines were observed after exposure to BSA solution ( Figure 12 ) and CuS-BSA nanosuspension ( Figure 13 ). The obtained results showed BSA to promote cell growth and proliferation, thus alleviating the cytotoxicity of the system ( Peng et al, 2013 ; Janani et al, 2021 ). A significant increasing trend was observed above the BSA concentration of 25 μg/ml in all studied lines, except HCT116 where at the maximum studied BSA concentration of 100 μg/ml a slight onset of inhibition of metabolic activity was observed (decrease by 9%).…”

Section: Resultsmentioning

confidence: 89%

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

et al. 2022

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The biocompatible nanosuspension of CuS nanoparticles (NPs) using bovine serum albumin (BSA) as a capping agent was prepared using a two-stage mechanochemical approach. CuS NPs were firstly synthetized by a high-energy planetary ball milling in 15 min by milling elemental precursors. The stability of nanoparticles in the simulated body fluids was studied, revealing zero copper concentration in the leachates, except simulated lung fluid (SLF, 0.015%) and simulated gastric fluid (SGF, 0.078%). Albumin sorption on CuS NPs was studied in static and dynamic modes showing a higher kinetic rate for the dynamic mode. The equilibrium state of adsorption was reached after 90 min with an adsorption capacity of 86 mg/g compared to the static mode when the capacity 59 mg/g was reached after 2 h. Then, a wet stirred media milling in a solution of BSA was introduced to yield the CuS-BSA nanosuspension, being stable for more than 10 months, as confirmed by photon cross-correlation spectroscopy. The fluorescent properties of the nanosuspension were confirmed by photoluminescence spectroscopy, which also showed that tryptophan present in the BSA could be closer to the binding site of CuS than the tyrosine residue. The biological activity was determined by in vitro tests on selected cancer and non-tumor cell lines. The results have shown that the CuS-BSA nanosuspension inhibits the metabolic activity of the cells as well as decreases their viability upon photothermal ablation.

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Section: Resultsmentioning

confidence: 89%

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

et al. 2022

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“…Comparatively, the conduction band (CB) position of BTO was more negative, and the valence band (VB) position of BO was more positive. Hence, according to the fact that pure BTO and BO both were p-type conductivity, it can be concluded that the produced BBTO composite was an interleaved type II and p–p heterojunction. ,,− Such energy band arrangement favored the separation of carriers in BBTO and promoted the photocatalytic efficiency. , …”

Section: Resultsmentioning

confidence: 96%

Preparation of Binary Type II α-Bi₂O₃/Bi₁₂TiO₂₀ Cross-Shaped Heterojunction with Enhanced Visible Light Photocatalytic Performance

Wang

Yan

Sun

et al. 2022

ACS Appl. Electron. Mater.

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The binary type II α-Bi 2 O 3 /Bi 12 TiO 20 heterojunction composite was successfully obtained by a simple hydrothermal process. The pH value and the Bi/Ti molar ratio were the key to form the α-Bi 2 O 3 /Bi 12 TiO 20 heterojunction. The α-Bi 2 O 3 nanobelts intertwined with the Bi 12 TiO 20 nanobelts to form a cross-shaped structure in the composite. The obvious red-shift can be detected in Raman spectra. UV−vis DRS also revealed that the effectively reduced band gap of the heterojunction composite led to sunlight absorption in a larger range. Meanwhile, the photocurrent responses and the EIS demonstrated that the combination of Bi 12 TiO 20 and α-Bi 2 O 3 significantly promoted the separation of photogenerated electron−hole pairs and suppressed their recombination, which was also confirmed by PL analysis. Furthermore, photocatalytic degradation experiments proved that the α-Bi 2 O 3 / Bi 12 TiO 20 heterojunction composite had excellent catalytic performance under visible light, whose rate constant value (k) was about 2.0 times that of pure α-Bi 2 O 3 and 4.5 times that of pure Bi 12 TiO 20 samples. A possible photocatalytic mechanism of α-Bi 2 O 3 /Bi 12 TiO 20 was also proposed based on p−p and type II heterojunction. It was concluded that the heterojunction structure markedly promoted the migration and separation of carriers to enhance its photoactivity.

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“…A recent report ( Janani et al, 2021 ), has detailed the influence of PC on the toxicity of ZnONPs in A. cepa . The ZnONPs with PC exhibited less cytotoxicity, chromosomal aberrations and micronuclei formation compared to ZnONPs without PC.…”

Section: Resultsmentioning

confidence: 99%

Reduced Genotoxicity of Gold Nanoparticles With Protein Corona in Allium cepa

Arya

Rookes

Cahill

et al. 2022

Front. Bioeng. Biotechnol.

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Increased usage of gold nanoparticles (AuNPs) in biomedicine, biosensing, diagnostics and cosmetics has undoubtedly facilitated accidental and unintentional release of AuNPs into specific microenvironments. This is raising serious questions concerning adverse effects of AuNPs on off-target cells, tissues and/or organisms. Applications utilizing AuNPs will typically expose the nanoparticles to biological fluids such as cell serum and/or culture media, resulting in the formation of protein corona (PC) on the AuNPs. Evidence for PC altering the toxicological signatures of AuNPs is well studied in animal systems. In this report, we observed significant genotoxicity in Allium cepa root meristematic cells (an off-target bioindicator) treated with high concentrations (≥100 µg/ml) of green-synthesized vanillin capped gold nanoparticles (VAuNPs). In contrast, protein-coated VAuNPs (PC-VAuNPs) of similar concentrations had negligible genotoxic effects. This could be attributed to the change in physicochemical characteristics due to surface functionalization of proteins on VAuNPs and/or differential bioaccumulation of gold ions in root cells. High elemental gold accumulation was evident from µ-XRF mapping in VAuNPs-treated roots compared to treatment with PC-VAuNPs. These data infer that the toxicological signatures of AuNPs are influenced by the biological route that they follow to reach off-target organisms such as plants. Hence, the current findings highlight the genotoxic risk associated with AuNPs, which, due to the enhanced utility, are emerging as new pollutants. As conflicting observations on the toxicity of green-synthesized AuNPs are increasingly reported, we recommend that detailed studies are required to investigate the changes in the toxicological signatures of AuNPs, particularly before and after their interaction with biological media and systems.

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Impact of bovine serum albumin – A protein corona on toxicity of ZnO NPs in environmental model systems of plant, bacteria, algae and crustaceans

Cited by 30 publications

References 80 publications

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

Preparation of Binary Type II α-Bi₂O₃/Bi₁₂TiO₂₀ Cross-Shaped Heterojunction with Enhanced Visible Light Photocatalytic Performance

Reduced Genotoxicity of Gold Nanoparticles With Protein Corona in Allium cepa

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Impact of bovine serum albumin – A protein corona on toxicity of ZnO NPs in environmental model systems of plant, bacteria, algae and crustaceans

Cited by 30 publications

References 80 publications

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

Preparation of Binary Type II α-Bi2O3/Bi12TiO20 Cross-Shaped Heterojunction with Enhanced Visible Light Photocatalytic Performance

Reduced Genotoxicity of Gold Nanoparticles With Protein Corona in Allium cepa

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Preparation of Binary Type II α-Bi₂O₃/Bi₁₂TiO₂₀ Cross-Shaped Heterojunction with Enhanced Visible Light Photocatalytic Performance