Impact of biodiesel on biodeterioration of stored Brazilian diesel oil

Bücker, Francielle; Santestevan, Naiara Aguiar; Roesch, Luiz Fernando Würdig; Jacques, Rodrigo Josemar Seminotti; Peralba, Maria do Carmo Ruaro; Camargo, Flávio Anastácio de Oliveira; Bento, Fátima Menezes

doi:10.1016/j.ibiod.2010.09.008

Cited by 85 publications

(60 citation statements)

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“…Many factors, such as the presence of water in the bottom of the tanks during storage, have been cited as increasing microbial growth in the systems and can lead to blocking of pipelines and filters, affecting the final quality of the fuel and corrosion of the tanks (Bento and Gaylarde, 2001;Bento et al, 2004;Bücker et al, 2011;White et al, 2011;Cazarolli et al, 2012Cazarolli et al, , 2014Zimmer et al, 2013;Passman, 2013). A concentration of only 1% water in a storage system is sufficient for the growth of aerobic and anaerobic bacteria and yeasts, as well as for the development of fungal biomass at the oil/water interface (Gaylarde et al, 1999;Chesneau, 2000;Bento and Gaylarde, 2001;Bento et al, 2004;Bücker et al, 2011;Sørensen et al, 2011;Cazarolli et al, 2012Cazarolli et al, , 2014Zimmer et al, 2013;Passman, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…A concentration of only 1% water in a storage system is sufficient for the growth of aerobic and anaerobic bacteria and yeasts, as well as for the development of fungal biomass at the oil/water interface (Gaylarde et al, 1999;Chesneau, 2000;Bento and Gaylarde, 2001;Bento et al, 2004;Bücker et al, 2011;Sørensen et al, 2011;Cazarolli et al, 2012Cazarolli et al, , 2014Zimmer et al, 2013;Passman, 2013). Numerous microorganisms have been isolated from fuels (Atlas, 1981;Gaylarde et al, 1999;Bento and Gaylarde, 2001;Bento et al, 2004;Bücker et al, 2011;White et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Growth of Paecilomyces variotii in B0 (diesel), B100 (biodiesel) and B7 (blend), degradation and molecular detection

et al. 2015

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The introduction of biodiesel to diesel may allow the fuel to be more susceptible to microorganism growth, especially during incorrect storage. To evaluate the effect of adding biodiesel in pure diesel on the growth of Paecilomyces variotii, microcosms containing pure diesel (B0), blend diesel/biodiesel (B7) and pure biodiesel (B100) were used. In microcosm with minimal mineral medium and B0, B7 or B100, after 60 days, the biomass (dry weight) formed at interface oil-water in B7 and B100 was significantly higher when compared to that of B0. Infrared analysis showed reduction of the carbonile fraction in B7 and B100 suggesting formation of intermediate compounds in B7. To monitor possible contamination of fuel storage tank by P. variotii samples were collected and analysed by specific-PCR assay for detection of P. variotii spores in the aqueous phase. This method was able to detect a minimum of 10 3 spores ml -1 , corresponding to 0.0144 ng µl -1 of DNA. Specificity was tested against Aspergillus fumigatus and Pseudallescheria boydii.Keywords: biodiesel, Paecilomyces variotii, deteriogenic, biodegradation, infrared spectroscopy, PCR.Crescimento de Paecilomyces variotii em B0 (diesel), B100 (biodiesel) e B7 (mistura), degradação e detecção molecular Resumo A introdução de biodiesel ao diesel pode permitir que o combustível se torne mais suscetível ao crescimento de microorganismos, especialmente durante o armazenamento incorreto. Para analisar o efeito da adição de biodiesel em diesel puro no crescimento de Paecilomyces variotii, avaliou-se seu desenvolvimento em microcosmos contendo diesel puro (B0), mistura diesel/biodiesel (B7) e biodiesel puro (B100). Em microcosmos com meio mineral mínimo e B0, B7 ou B100, após 60 dias, a biomassa (peso seco) formada na interface óleo-agua com B7 e B100 foi significativamente maior quando comparada com a de B0. A análise de infravermelho mostrou redução da fração carbonila em B7 e B100, sugerindo a formação de compostos intermediários em B7. Para monitorar uma possível contaminação de tanque de armazenamento de combustível por P. variotii, amostras foram colhidas e analisadas por um teste de PCR específico para detecção de esporos deste fungo em fase aquosa. Este método foi capaz de detectar um mínimo de 10 3 esporos ml -1, correspondente a 0.0144 ng µl -1 de DNA. Especificidade foi testada contra Aspergillus fumigatus e Pseudallescheria boydii.Palavras-chave: biodiesel, Paecilomyces variotii, deteriogênico, biodegradação, espectroscopia de infravermelho, PCR.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Growth of Paecilomyces variotii in B0 (diesel), B100 (biodiesel) and B7 (blend), degradation and molecular detection

et al. 2015

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“…The impurities (non-esters components) of the A. aculeata pulp and J. curcas biodiesels investigated herein stood at 18,55±0,88% and 4,15±0,15% of, respectively. Therefore, it could be concluded that these impurities, such as glycerol, or mono, di-and triacylglycerides (resulted from low transesterification yield and/or a poor purification process), might have been consumed by fungi and thus could have contributed to the increased susceptibility of biodiesel to microbial contamination (Bücker et al, 2011;Cazarolli et al, 2014). Vieira et al (2006) observed the degradation of palm oil by an unidentified microbial isolate and obtained the following degradation profile of methyl esters; myristic acid by 31.67%, palmitic acid by 51.28%, stearic acid by 34.11%, oleic acid by 33.31%, and linoleic acid by 25.16%.…”

Section: Biodiesel Biodegradationmentioning

confidence: 99%

Microbial growth in Acrocomia aculeata pulp oil, Jatropha curcas oil, and their respective biodiesels under simulated storage conditions

et al. 2016

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“…For most of the investigations carried out at the bench scale, it was found that microorganisms easily biodegrade biodiesel and blends higher than B10 compared to fossil diesel; assessing in the laboratory the ability of fungi to biodegrade soy-derived blended diesel, Bucker et al (2011) found that the blend containing a higher proportion of vegetable oil biodiesel was much susceptible to biodegradation. A similar trend was observed by Meyer et al (2014), who found that the presence of biodiesel increases the biodegradation of petroleum diesel.…”

Section: Introductionmentioning

confidence: 99%

Adaptation behaviour of bacterial species and impact on the biodegradation of biodiesel-diesel

Fosso‐Kankeu

Marx

Brink

2017

Braz. J. Chem. Eng.

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-Two bacteria, namely Bacillus subtilis and Pseudomonas aeruginosa were exposed to different concentrations of diesel to increase their adaptation to the fossil fuel, and were used for the degradation of diesel-biodiesel blends. The biodegradation rate was evaluated using the redox indicator 2,6-dichlorophenol indophenol (DCPIP) test and gravimetric analysis. The preliminary exposure of cells to diesel proved to improve their biodegradation capacities, and exposure to a higher concentration (70%) of diesel resulted in maximum biodegradation of up to 58.38 g and 1.78 g of degraded oil per CFU/mL (10 10 ) for P. aeruginosa and B. subtilis respectively. It was found that the adapted cells preferably used diesel in the blend. P. aeruginosa and B. subtilis exhibited different adaptation capabilities and biodegradation behaviour. Biodiesel stimulated the biodegradation of the diesel-biodiesel blends by non-adapted cells only; the adapted cells exhibited a different behaviour.

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Impact of biodiesel on biodeterioration of stored Brazilian diesel oil

Cited by 85 publications

References 29 publications

Growth of Paecilomyces variotii in B0 (diesel), B100 (biodiesel) and B7 (blend), degradation and molecular detection

Growth of Paecilomyces variotii in B0 (diesel), B100 (biodiesel) and B7 (blend), degradation and molecular detection

Microbial growth in Acrocomia aculeata pulp oil, Jatropha curcas oil, and their respective biodiesels under simulated storage conditions

Adaptation behaviour of bacterial species and impact on the biodegradation of biodiesel-diesel

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