Impact of bioaugmentation on biochemical methane potential for wheat straw with addition of Clostridium cellulolyticum

Peng, Xiaowei; Börner, Rosa Aragão; Nges, Ivo Achu; Liu, Jing

doi:10.1016/j.biortech.2013.11.067

Cited by 83 publications

(33 citation statements)

References 16 publications

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“…Moreover, closely related members of R . cellulolyticum have been detected in anaerobic digesters treating waste with high cellulose content [14] and the species has recently been shown to improve wheat straw methanization by bioaugmentation [15]. Finally, the wild-type R .…”

Section: Introductionmentioning

confidence: 99%

Whole Proteome Analyses on Ruminiclostridium cellulolyticum Show a Modulation of the Cellulolysis Machinery in Response to Cellulosic Materials with Subtle Differences in Chemical and Structural Properties

Badalato¹,

Guillot

Sabarly³

et al. 2017

PLoS ONE

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Lignocellulosic materials from municipal solid waste emerge as attractive resources for anaerobic digestion biorefinery. To increase the knowledge required for establishing efficient bioprocesses, dynamics of batch fermentation by the cellulolytic bacterium Ruminiclostridium cellulolyticum were compared using three cellulosic materials, paper handkerchief, cotton discs and Whatman filter paper. Fermentation of paper handkerchief occurred the fastest and resulted in a specific metabolic profile: it resulted in the lowest acetate-to-lactate and acetate-to-ethanol ratios. By shotgun proteomic analyses of paper handkerchief and Whatman paper incubations, 151 proteins with significantly different levels were detected, including 20 of the 65 cellulosomal components, 8 non-cellulosomal CAZymes and 44 distinct extracytoplasmic proteins. Consistent with the specific metabolic profile observed, many enzymes from the central carbon catabolic pathways had higher levels in paper handkerchief incubations. Among the quantified CAZymes and cellulosomal components, 10 endoglucanases mainly from the GH9 families and 7 other cellulosomal subunits had lower levels in paper handkerchief incubations. An in-depth characterization of the materials used showed that the lower levels of endoglucanases in paper handkerchief incubations could hypothetically result from its lower crystallinity index (50%) and degree of polymerization (970). By contrast, the higher hemicellulose rate in paper handkerchief (13.87%) did not result in the enhanced expression of enzyme with xylanase as primary activity, including enzymes from the “xyl-doc” cluster. It suggests the absence, in this material, of molecular structures that specifically lead to xylanase induction. The integrated approach developed in this work shows that subtle differences among cellulosic materials regarding chemical and structural characteristics have significant effects on expressed bacterial functions, in particular the cellulolysis machinery, resulting in different metabolic patterns and degradation dynamics.

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Section: Introductionmentioning

confidence: 99%

Whole Proteome Analyses on Ruminiclostridium cellulolyticum Show a Modulation of the Cellulolysis Machinery in Response to Cellulosic Materials with Subtle Differences in Chemical and Structural Properties

Badalato¹,

Guillot

Sabarly³

et al. 2017

PLoS ONE

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“…In most continuous operation cases however, the improvement in methane production was not sustained over time, most likely due to wash-out of the bioaugmented microorganisms that have to compete with the indigenous microbial community (Mladenovska et al, 2001;Nielsen et al, 2007). As noted by Peng et al (2013), although bioaugmentation with cellulolytic microorganisms has been shown to improve digestion performance, the ability to maintain a stable bioaugmented microbial community in a continuous process has been a significant challenge. Therefore, this study sought to investigate the benefits of routine bioaugmentation, consisting of regular repeated additions of a cellulolytic bioculture, as an alternative to one-time bioaugmentation, and as a method for achieving a stable and more effective microbial community for anaerobic digestion of a cellulosic feedstock.…”

mentioning

confidence: 95%

Improving anaerobic digestion of a cellulosic waste via routine bioaugmentation with cellulolytic microorganisms

Martin-Ryals

Schideman

et al. 2015

Bioresource Technology

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“…This has been demonstrated for mixed hemicellulolytic bacteria cultures [69,70] and isolated bacterial species obtained from natural biogas-producing consortia as well, i.e. hydrogen-producing cultures of Caldicellulosiruptor saccharolyticus and Enterobacter cloacae [71,72] or Clostridium cellulolyticum, which was successfully adopted to enhance the hydrolysis of wheat straw leading to increased BMP tests improving the utilization of lignocellulosic substrates [73].…”

Section: Enzyme Tests and Applicationsmentioning

confidence: 98%

Microbiology and Molecular Biology Tools for Biogas Process Analysis, Diagnosis and Control

Lebuhn

Weiß

Munk

et al. 2015

Biogas Science and Technology

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Many biotechnological processes such as biogas production or defined biotransformations are carried out by microorganisms or tightly cooperating microbial communities. Process breakdown is the maximum credible accident for the operator. Any time savings that can be provided by suitable early-warning systems and allow for specific countermeasures are of great value. Process disturbance, frequently due to nutritional shortcomings, malfunction or operational deficits, is evidenced conventionally by process chemistry parameters. However, knowledge on systems microbiology and its function has essentially increased in the last two decades, and molecular biology tools, most of which are directed against nucleic acids, have been developed to analyze and diagnose the process. Some of these systems have been shown to indicate changes of the process status considerably earlier than the conventionally applied process chemistry parameters. This is reasonable because the triggering catalyst is determined, activity changes of the microbes that perform the reaction. These molecular biology tools have thus the potential to add to and improve the established process diagnosis system. This chapter is dealing with the actual state of the art of biogas process analysis in practice, and introduces molecular biology tools that have been shown to be of particular value in complementing the current systems of process monitoring and diagnosis, with emphasis on nucleic acid targeted molecular biology systems.

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Impact of bioaugmentation on biochemical methane potential for wheat straw with addition of Clostridium cellulolyticum

Cited by 83 publications

References 16 publications

Whole Proteome Analyses on Ruminiclostridium cellulolyticum Show a Modulation of the Cellulolysis Machinery in Response to Cellulosic Materials with Subtle Differences in Chemical and Structural Properties

Whole Proteome Analyses on Ruminiclostridium cellulolyticum Show a Modulation of the Cellulolysis Machinery in Response to Cellulosic Materials with Subtle Differences in Chemical and Structural Properties

Improving anaerobic digestion of a cellulosic waste via routine bioaugmentation with cellulolytic microorganisms

Microbiology and Molecular Biology Tools for Biogas Process Analysis, Diagnosis and Control

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