The development of terrestrial laser scanning (TLS) has opened new avenues in the study of trees. Although TLS provides valuable information on structural elements, fine-scale analysis, e.g., at the annual shoots (AS) scale, is currently not possible. We present a new model to segment and classify AS from tree skeletons into a finite set of “physiological ages” (i.e., state of specialization and physiological age (PA)). When testing the model against perfect data, 90% of AS year and 99% of AS physiological ages were correctly extracted. AS length-estimated errors varied between 0.39 cm and 2.57 cm depending on the PA. When applying the model to tree reconstructions using real-life simulated TLS data, 50% of the AS and 77% of the total tree length are reconstructed. Using an architectural automaton to deal with non-reconstructed short axes, errors associated with AS number and length were reduced to 5% and 12%, respectively. Finally, the model was applied to real trees and was consistent with previous findings obtained from manual measurements in a similar context. This new method could be used for determining tree phenotype or for analyzing tree architecture.