Impact of bacterial persisters on their host

Moldoveanu, Ana Laura; Rycroft, Julian Anthony; Hélaine, Sophie

doi:10.1016/j.mib.2020.07.006

Cited by 32 publications

(33 citation statements)

References 50 publications

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“…Moreover, although persisters were originally described as dormant ( 24 ), recent studies have questioned this state ( 25 – 27 ), especially in intracellular niches, where persisters generally sustain metabolic activity to withstand host-specific stresses ( 28 , 29 ), pointing to a crucial role of the environment to modulate their metabolism.…”

Section: Introductionmentioning

confidence: 99%

Host Cell Oxidative Stress Induces Dormant Staphylococcus aureus Persisters

Peyrusson

Nguyen

Najdovski³

et al. 2022

Microbiol Spectr

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By their capacity to survive to antibiotic pressure and to regrow and give rise to a susceptible population once this pressure is relieved, intracellular persisters of S. aureus may contribute to explain therapeutic failures and recurrent infections. Here, we show that the level of dormancy and the subsequent capacity to resuscitate from this resting state are dependent on the level of oxidative stress in the host cells where bacteria survive.

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Section: Introductionmentioning

confidence: 99%

Host Cell Oxidative Stress Induces Dormant Staphylococcus aureus Persisters

Peyrusson

Nguyen

Najdovski³

et al. 2022

Microbiol Spectr

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“…Examples of phenotypic variation are the “bet‐hedging” and “division of labor” survival strategies, which allow the distribution of risk and gain of function at the population level by constantly developing bacterial isogenic subpopulations with different features (Diard et al , 2013 ; Arnoldini et al , 2014 ; Carcamo‐Oyarce et al , 2015 ; Grimbergen et al , 2015 ; Laventie et al , 2019 ). A prominent and clinically relevant example of phenotypic heterogeneity is the emergence of non‐growing antibiotic persisters in a genetically identical bacterial population (Balaban et al , 2004 ; Hélaine et al , 2014 ; Brauner et al , 2016 ; Conlon et al , 2016 ; Harms et al , 2016 ; Kortebi et al , 2017 ; Personnic et al , 2019b ; Moldoveanu et al , 2021 ). Extensive cell‐to‐cell variability has been also observed in infected tissues, where genetically identical pathogens deal with structured micro‐environments and components of the immune system in a phenotypically heterogeneous manner (Burton et al , 2014 ; Claudi et al , 2014 ; Davis et al , 2015 ; Manina et al , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Quorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole periphery

et al. 2021

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The Gram‐negative bacterium Legionella pneumophila is the causative agent of Legionnaires' disease and replicates in amoebae and macrophages within a distinct compartment, the Legionella‐containing vacuole (LCV). The facultative intracellular pathogen switches between a replicative, non‐virulent and a non‐replicating, virulent/transmissive phase. Here, we show on a single‐cell level that at late stages of infection, individual motile (PflaA‐GFP‐positive) and virulent (PralF‐ and PsidC‐GFP‐positive) L. pneumophila emerge in the cluster of non‐growing bacteria within an LCV. Comparative proteomics of PflaA‐GFP‐positive and PflaA‐GFP‐negative L. pneumophila subpopulations reveals distinct proteomes with flagellar proteins or cell division proteins being preferentially produced by the former or the latter, respectively. Toward the end of an infection cycle (˜ 48 h), the PflaA‐GFP‐positive L. pneumophila subpopulation emerges at the cluster periphery, predominantly escapes the LCV, and spreads from the bursting host cell. These processes are mediated by the Legionella quorum sensing (Lqs) system. Thus, quorum sensing regulates the emergence of a subpopulation of transmissive L. pneumophila at the LCV periphery, and phenotypic heterogeneity underlies the intravacuolar bi‐phasic life cycle of L. pneumophila.

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“…Bacteria embedded in the deep layers of biofilm are often metabolically inactive (dormant), which may be a result of low oxygen-density and transcriptional changes to adapt to the nutrient-scarce community-based lifestyle. This state of dormancy also poses and important hurdle for therapy, as many drugs (especially bactericidal agents) require the active division of bacterial cells to be effective [63]. As E. coli is one of the most common nosocomial pathogen (especially in catheter-associated UTIs, corresponding to >50% of cases), biofilm-production is of critical importance for its persistence, pathogenicity and survival [64,65].…”

Section: Discussion Review Of the Literaturementioning

confidence: 99%

Association between biofilm-production and antibiotic resistance in Escherichia coli isolates: A laboratory-based case study and a literature review

Gajdács

Kárpáti

Nagy

et al. 2021

AMicr

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Bacteria can enhance their survival by attaching to inanimate surfaces or tissues, and presenting as multicellular communities encased in a protective extracellular matrix called biofilm. There has been pronounced interest in assessing the relationship between the antibiotic resistant phenotype and biofilm-production in clinically-relevant pathogens. The aim of the present paper was to provide additional experimental results on the topic, testing the biofilm-forming capacity of Escherichia coli isolates using in vitro methods in the context of their antibiotic resistance in the form of a laboratory case study, in addition to provide a comprehensive review of the subject. In our case study, a total of two hundred and fifty (n = 250) E. coli isolates, originating from either clean-catch urine samples (n = 125) or invasive samples (n = 125) were included. The colony morphology of isolates were recorded after 24h, while antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method. Biofilm-formation of the isolates was assessed with the crystal violet tube-adherence method. Altogether 57 isolates (22.8%) isolates were multidrug resistant (MDR), 89 isolates (35.6%) produced large colonies (>3 mm), mucoid variant colonies were produced in 131 cases (52.4%), and 108 (43.2%) were positive for biofilm formation. Biofilm-producers were less common among isolates resistant to third-generation cephalosporins and trimethoprim-sulfamethoxazole (P = 0.043 and P = 0.023, respectively). Biofilms facilitate a protective growth strategy in bacteria, ensuring safety against environmental stressors, components of the immune system and noxious chemical agents. Being an integral part of bacterial physiology, biofilm-formation is interdependent with the expression of other virulence factors (especially adhesins) and quorum sensing signal molecules. More research is required to allow for the full understanding of the interplay between the MDR phenotype and biofilm-production, which will facilitate the development of novel therapeutic strategies.

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Impact of bacterial persisters on their host

Cited by 32 publications

References 50 publications

Host Cell Oxidative Stress Induces Dormant Staphylococcus aureus Persisters

Host Cell Oxidative Stress Induces Dormant Staphylococcus aureus Persisters

Quorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole periphery

Association between biofilm-production and antibiotic resistance in Escherichia coli isolates: A laboratory-based case study and a literature review

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