Impact of bacterial endotoxin on the structure of DMPC membranes

Nagel, Michael G.; Brauckmann, Stephan; Moegle-Hofacker, Franzeska; Effenberger-Neidnicht, Katharina; Hartmann, Matthias; Groot, Herbert de; Mayer, Christian

doi:10.1016/j.bbamem.2015.06.008

Cited by 8 publications

(8 citation statements)

References 31 publications

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“…These findings offer decisive support to previous reports on the ordering of lipid membranes by E. coli LPS [119,120,121,122,123]. LPS was shown to insert into 1,2-dimyristoyl- sn -glycero-3-phosphocholine (DMPC) bilayers, prompting significant reduction of the elastic strength, subsequently reducing vesicles size [124].…”

Section: Trp Channel Modulation By the Natural Membrane Modulator supporting

confidence: 87%

TRP Channels as Sensors of Chemically-Induced Changes in Cell Membrane Mechanical Properties

Startek

Boonen

Talavera

et al. 2019

IJMS

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Transient Receptor Potential ion channels (TRPs) have been described as polymodal sensors, being responsible for transducing a wide variety of stimuli, and being involved in sensory functions such as chemosensation, thermosensation, mechanosensation, and photosensation. Mechanical and chemical stresses exerted on the membrane can be transduced by specialized proteins into meaningful intracellular biochemical signaling, resulting in physiological changes. Of particular interest are compounds that can change the local physical properties of the membrane, thereby affecting nearby proteins, such as TRP channels, which are highly sensitive to the membrane environment. In this review, we provide an overview of the current knowledge of TRP channel activation as a result of changes in the membrane properties induced by amphipathic structural lipidic components such as cholesterol and diacylglycerol, and by exogenous amphipathic bacterial endotoxins.

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Section: Trp Channel Modulation By the Natural Membrane Modulator supporting

confidence: 87%

TRP Channels as Sensors of Chemically-Induced Changes in Cell Membrane Mechanical Properties

Startek

Boonen

Talavera

et al. 2019

IJMS

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“…coli LPS 14 , 40 – 43 . This endotoxin was shown to rapidly integrate into 1,2-dimyristoyl- sn -glycero-3-phosphocholine (DMPC) membranes, inducing significant reduction of the elastic strength, resulting in vesicles size reduction 44 . Correspondingly, E .…”

Section: Discussionmentioning

confidence: 99%

Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

Startek

Talavera

Voets

et al. 2018

Sci Rep

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Bacterial lipopolysaccharides (LPS) activate the TRPA1 cation channels in sensory neurons, leading to acute pain and inflammation in mice and to aversive behaviors in fruit flies. However, the precise mechanisms underlying this effect remain elusive. Here we assessed the hypothesis that TRPA1 is activated by mechanical perturbations induced upon LPS insertion in the plasma membrane. We asked whether the effects of different LPS on TRPA1 relate to their ability to induce mechanical alterations in artificial and cellular membranes. We found that LPS from E. coli, but not from S. minnesota, activates TRPA1. We then assessed the effects of these LPS on lipid membranes using dyes whose fluorescence properties change upon alteration of the local lipid environment. E. coli LPS was more effective than S. minnesota LPS in shifting Laurdan’s emission spectrum towards lower wavelengths, increasing the fluorescence anisotropy of diphenylhexatriene and reducing the fluorescence intensity of merocyanine 540. These data indicate that E. coli LPS induces stronger changes in the local lipid environment than S. minnesota LPS, paralleling its distinct ability to activate TRPA1. Our findings indicate that LPS activate TRPA1 by producing mechanical perturbations in the plasma membrane and suggest that TRPA1-mediated chemosensation may result from primary mechanosensory mechanisms.

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“…The intercalation of LPS with lipid membranes is well known 11 12 13 . We recently showed, that LPS molecules quickly insert into phospholipid bilayers, increase membrane fluctuation amplitudes, and significantly weaken their mechanical stiffness 14 . In experiments with LPS from K. pneumonia , S. enterica , and E. coli , Ciesielski et al .…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide-induced hemolysis: Evidence for direct membrane interactions

Brauckmann

Effenberger-Neidnicht

Groot

et al. 2016

Sci Rep

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While hemolysis in patients with sepsis is associated with increased mortality its mechanisms are unknown and Toll-like receptor (TLR)-4 mediated effects, complement-mediated hemolysis, or direct cell membrane effects are all conceivable mechanisms. In this study, we tested the hypotheses that toxic lipopolysaccharide (LPS) as well as non-toxic RS-LPS evokes hemolysis (1) by direct membrane effects, and (2) independent of the complement system and TLR-4 activation. We found, that incubation with LPS resulted in a marked time and concentration dependent increase of free hemoglobin concentration and LDH activity in whole blood and washed red cells. Red cell integrity was diminished as shown by decreased osmotic resistance, formation of schistocytes and rolls, and a decrease in red cell membrane stiffness. Non-toxic RS-LPS inhibited the LPS-evoked increase in TNF-α concentration demonstrating its TLR-4 antagonism, but augmented LPS-induced increase in supernatant hemoglobin concentration and membrane disturbances. Removal of plasma components in washed red cell assays failed to attenuate hemolysis. In summary, this study demonstrates direct physicochemical interactions of LPS with red cell membranes resulting in hemolysis under in vitro conditions. It might thus be hypothesized, that not all effects of LPS are mediated by TLR and may explain LPS toxicity in cells missing TLR.

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Impact of bacterial endotoxin on the structure of DMPC membranes

Cited by 8 publications

References 31 publications

TRP Channels as Sensors of Chemically-Induced Changes in Cell Membrane Mechanical Properties

TRP Channels as Sensors of Chemically-Induced Changes in Cell Membrane Mechanical Properties

Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

Lipopolysaccharide-induced hemolysis: Evidence for direct membrane interactions

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