Impact of Azospirillum sp. B510 on the Rhizosphere Microbiome of Rice under Field Conditions

Abstract

There has been increasing attention toward the influence of biofertilizers on the composition of microbial communities associated with crop plants. We investigated the impact of Azospirillum sp. B510, a bacterial strain with nitrogen-fixing ability, on the structure of bacterial and fungal communities within rice plant rhizospheres by amplicon sequencing at two sampling stages (the vegetative and harvest stages of rice). Principal coordinate analysis (PCoA) demonstrated a significant community shift in the bacterial microbiome when the plants were inoculated with B510 at the vegetative stage, which was very similar to the effect of chemical N-fertilizer application. This result suggested that the inoculation with B510 strongly influenced nitrogen uptake by the host plants under low nitrogen conditions. Least discriminant analysis (LDA) showed that the B510 inoculation significantly increased the N2-fixing Clostridium, Aeromonas and Bacillus populations. In contrast, there was no apparent influence of B510 on the fungal community structure. The putative functional properties of bacteria were identified through PICRUSt2, and this hinted that amino acid, sugar and vitamin production might be related to B510 inoculation. Our results indicate that B510 inoculation influenced the bacterial community structure by recruiting other N2-fixing bacteria in the absence of nitrogen fertilizer.

Performance of Halo-Alkali-Tolerant Endophytic Bacteria on Hybrid Pennisetum and Bacterial Community under Varying Soil Conditions

“…The inoculation with Penicillium oxalicum P66 and Aspergillus niger P39 increased bacterial communities in the rhizospheres of soybean (Glycine max Merr. 'Heinong 35') and maize (Zea mays L. 'Haiyu 6') [44]. The inoculation of Azospirillum sp.…”

Microrganismos promotores de crescimento de plantas - Trichoderma e Azospirillum: otimizando a cultura do milho em sistemas integrados de produção agropecuária

“…As bactérias do gênero Azospirillum são conhecidas por sua capacidade de fixação biológica de nitrogênio (FBN) atmosférico (ZEFFA et al, 2019), habilidade esta que permite que essas bactérias convertam o N do ar em uma forma utilizável pelas plantas, promovendo suprimento adicional de N para o crescimento e desenvolvimento dos vegetais, sendo particularmente benéfico para plantas gramíneas, como as culturas do milho, trigo, arroz (Oryza sativa L.) e brachiaria (Urochloa brizanta cv. Marandu), por exemplo, resultando, por conseguinte, na redução da necessidade de fertilizantes nitrogenados sintéticos (ZEFFA et al, 2019;GALINDO et al, 2022). Outro benefício para as plantas é a produção de fitohormônios que promove o estímulo para o desenvolvimento radicular, possibilitando com isso uma melhor absorção de nutrientes e maior resistência das plantas ao estresse hídrico (GALINDO et al, 2022;ZEFFA et al, 2019).…”

“…Marandu), por exemplo, resultando, por conseguinte, na redução da necessidade de fertilizantes nitrogenados sintéticos (ZEFFA et al, 2019;GALINDO et al, 2022). Outro benefício para as plantas é a produção de fitohormônios que promove o estímulo para o desenvolvimento radicular, possibilitando com isso uma melhor absorção de nutrientes e maior resistência das plantas ao estresse hídrico (GALINDO et al, 2022;ZEFFA et al, 2019).…”

Overview of biofertilizers in crop production and stress management for sustainable agriculture

“…Spirillum was found associated with the roots of the grain which were also capable of fixing nitrogen (Soumare et al, 2020 ). Azospirillum is gram-negative, non-nodulating, aerobic-associative nitrogen-fixing bacteria with plants having a C4 dicarboxylic pathway of photosynthesis, such as sugarcane, maize, sorghum, bajra, and cereals like wheat, rice, barley (Yasuda et al, 2022 ). They also produce cytokinin, gibberellins, and indole acetic acid, which aid in the uptake of N, P, and K and promote the growth of roots.…”