Impact of Automated Blood Culture Systems on the Management of Bloodstream Infections: Results from a Crossover Diagnostic Clinical Trial

Halperin, Ana; Polo, Juan Antonio Del Castillo; Cortés‐Cuevas, Jose Luís; Isasi, María José Cardenas; Morisaki, Mario Ampuero; Birch, Robert; Sánchez-Díaz, Ana Maria

doi:10.1128/spectrum.01436-22

Cited by 2 publications

(3 citation statements)

References 25 publications

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“…A significant proportion of positive blood cultures are associated with genuine bloodstream infections, emphasizing the importance of rapid pathogen identification to facilitate the early commencement of antimicrobial therapy. Such early intervention enhances the prospects for effective antimicrobial stewardship and optimizes patient management (6,7). During the study, various organisms were indicating a diverse microbiological landscape.…”

Section: Discussionmentioning

confidence: 99%

“…This system, along with others that utilize CO2-based detection via colorimetric or fluorescence methods, has improved the accuracy and timeliness of BSI detection. The integration of resin-containing media in culture bottles, such as the BacT/Alert FAN Plus, enhances the recovery of organisms, allowing for the effective pairing of aerobic and anaerobic bottles to capture a broad spectrum of pathogens (5). This study aims to assess the optimal incubation period for blood culture bottles in a high-tech diagnostic setup of a tertiary care hospital in Lahore.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Adequate Blood Culture Incubation Time to Accentuate Clinically Relevant Results from Blood Culture Instrument and Media System in a Tertiary Care Hospital Lahore

Aslam,

Farooque,

Malik

et al. 2024

JHRR

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Background: Bloodstream infections necessitate rapid identification and management. Automated blood culture systems have revolutionized diagnostic microbiology by expediting pathogen detection and enabling effective clinical interventions. This study assesses the optimal incubation period for blood cultures in a tertiary healthcare setting, aiming to enhance diagnostic accuracy and support antibiotic stewardship. Objective: To evaluate the adequacy of incubation times in blood cultures for producing clinically significant results using an automated system in a tertiary care setting. Methods: This cross-sectional descriptive study was conducted at the Microbiology Section of Combined Military Hospital, Lahore, from October 2022 to March 2023. All patient blood samples processed through the Bactec/Alert system were studied. Positive samples were cultured on Blood and MacConkey agar and incubated at 35 ± 2°C for 24 hours. Positivity rates, contamination, and patient demographics were analyzed using the Laboratory Information Management System. Results: Of the 8,216 blood culture bottles processed, 490 (5.96%) were flagged as positive. Early detection within the first 24 hours was noted in 41.63% (n=204) of cases, with subsequent detections on the second day at 58.65% (n=258), the third day at 5.30% (n=26), and the fourth day at 0.20% (n=1). The isolated pathogens included gram-positive organisms (16.32%, n=80), gram-negative organisms (80.20%, n=393), and Candida spp. (3.46%, n=17). Conclusion: Timely and effective blood culturing is crucial for the diagnostic confirmation of bacteremia, enabling appropriate pathogen identification and antimicrobial therapy. Shortening detection times can significantly impact clinical outcomes, reduce healthcare costs, and promote antimicrobial stewardship.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of Adequate Blood Culture Incubation Time to Accentuate Clinically Relevant Results from Blood Culture Instrument and Media System in a Tertiary Care Hospital Lahore

Aslam,

Farooque,

Malik

et al. 2024

JHRR

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“…Currently, the clinical "gold standard" for diagnosing bacteremia is a laboratory blood culture [4] . However, this method's significant drawback is its prolonged duration, taking about 5-7 days.…”

Section: Introductionmentioning

confidence: 99%

Bacterial distribution and predictive value of blood routine parameters in elderly patients with bacteremia: a cross-sectional study

Zhang,

Zhuo,

et al. 2023

Preprint

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to assess the distribution of bacteremia pathogens in elderly patients, to evaluate the impact of gender on pathogen distribution, and to determine the predictive value of routine blood parameters for diagnosing bacteremia. Methods: A total of 151 elderly patients (≥60 years old) who admitted to hospital from October 2022 to June 2023 were retrospectively studied. Routine blood test and blood culture were performed. ROC curve was used to analyze the diagnostic efficacy of blood routine parameters: white blood cell (WBC), neutrophil-to-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), red blood cell distribution width (RDW). Results: The subjects were categorized into either the culture-positive group (82 cases) and the culture-negative one (69 cases) according to blood culture results. There were no significant differences in age and gender between groups. The primary bacterial pathogens of bacteremia in the elderly were Escherichia coli, Klebsiella pneumoniae and Streptococcus. A significantly higher culture positivity rate for E. coli was found in elderly female patients compared to their male counterparts (P = 0.021). The area under the ROC curve of four parameters was: WBC, 0.851 (95% confidence interval (CI) 0.790 - 0.912); NLR, 0.919 (95% CI 0.875 - 0.963); PLR, 0.609 (95% CI 0.518 - 0.700); and RDW was 0.626 (95% CI 0.563 - 0.717). Conclusion: The most frequently pathogenic microorganism causing bacteremia was E. coli, with elderly female patients having a significantly higher rate of culture positivity. Routine blood parameters (WBC, NLR, PLR, and RDW) demonstrated predictive potential for bacteremia in elderly patients.

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Impact of Automated Blood Culture Systems on the Management of Bloodstream Infections: Results from a Crossover Diagnostic Clinical Trial

Abstract: Bloodstream infections are associated with high rates of morbidity and mortality. Blood culture remains the gold standard for the diagnosis of BSIs.

Cited by 2 publications

References 25 publications

Assessment of Adequate Blood Culture Incubation Time to Accentuate Clinically Relevant Results from Blood Culture Instrument and Media System in a Tertiary Care Hospital Lahore

Assessment of Adequate Blood Culture Incubation Time to Accentuate Clinically Relevant Results from Blood Culture Instrument and Media System in a Tertiary Care Hospital Lahore

Bacterial distribution and predictive value of blood routine parameters in elderly patients with bacteremia: a cross-sectional study

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