Impact evaluation of marking Salmo trutta with Alizarin Red S produced by different manufacturers

Search for the Substantiation of Reasonable Native Elemental Background Values and Reference Variables in Topsoil on Glaciogenic and Postglacial Deposits in a Vilnius Peri-Urban Area

“…The used RMs and CRMs were prepared pari passu with our analyzed topsoil samples. Earlier, we mentioned these procedures in a series of publications including [3,[56][57][58][59][60][61].…”

Staining protocols affect use of otolith to estimate the demography of the damselfish sergeant major (Abudefduf vaigiensis)

“…It is important to know whether marking affects somatic growth for experiments that attempt to both validate increment deposition and measure growth rates in laboratory settings. Despite the suggestion that ARS does not impact growth (Bashey, 2004;Liu et al, 2009), some researchers have indicated that growth inhibition occurs between different manufacturers (Jurgelėnė et al, 2022). As the use of stain extends beyond validation, such as bone mineralization research, fish performance, and fish stock programs, it is important to establish whether ARS impacts growth and physiological processes that may interfere with the interpretation of growth from calcified structures, and whether a lower concentration should be used to minimize any potential effects.…”

“…These toxicants have been shown to result in lethargic movement, disrupt startle response, and impair swimming performance, indicating a possible mechanism for stunting growth at high concentration (ibid). Given the high concentrations of contaminates found in ARS by Jurgelėnė et al (2022), the downstream effect on xenobiotic processes at high and medium concentrations should not be discounted. Physiological stress, such as chemical and physical changes, is well documented to have negative effects on fish growth and wellbeing (Barton, 2002).…”