Imorin: a sexual attractiveness pheromone in female red-bellied newts (Cynops pyrrhogaster)

Nakada, Tomoaki; Toyoda, Fumiyo; Matsuda, Kouhei; Nakakura, Takashi; Hasunuma, Itaru; Yamamoto, Kazutoshi; Onoue, Satomi; Yokosuka, Makoto; Kikuyama, Sakaé

doi:10.1038/srep41334

Cited by 26 publications

(14 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reproductive isolation, which restricts gene flow between sympatric populations, is one of the key mechanisms of speciation [1]. Mating between individuals of closely related species is prevented by a prezygotic barrier, which is mainly caused by changes in signals that enable individuals to appropriately recognize the opposite sex: for example, pheromones in insects [2,3] and amphibians [4,5], body color in fish [6], and song in birds [7]. Although such reproductive isolation has been frequently studied in higher organisms, far less is known in fungi [8].…”

Section: Introductionmentioning

confidence: 99%

Asymmetric diversification of mating pheromones in fission yeast

2019

View full text Add to dashboard Cite

In fungi, mating between partners depends on the molecular recognition of two peptidyl mating pheromones by their respective receptors. The fission yeast Schizosaccharomyces pombe (Sp) has two mating types, Plus (P) and Minus (M). The mating pheromones P-factor and M-factor, secreted by P and M cells, are recognized by the receptors mating type auxiliary minus 2 (Mam2) and mating type auxiliary plus 3 (Map3), respectively. Our recent study demonstrated that a few mutations in both M-factor and Map3 can trigger reproductive isolation in S. pombe. Here, we explored the mechanism underlying reproductive isolation through genetic changes of pheromones/receptors in nature. We investigated the diversity of genes encoding the pheromones and their receptor in 150 wild S. pombe strains. Whereas the amino acid sequences of M-factor and Map3 were completely conserved, those of P-factor and Mam2 were very diverse. In addition, the P-factor gene contained varying numbers of tandem repeats of P-factor (4–8 repeats). By exploring the recognition specificity of pheromones between S. pombe and its close relative Schizosaccharomyces octosporus (So), we found that So-M-factor did not have an effect on S. pombe P cells, but So-P-factor had a partial effect on S. pombe M cells. Thus, recognition of M-factor seems to be stringent, whereas that of P-factor is relatively relaxed. We speculate that asymmetric diversification of the two pheromones might be facilitated by the distinctly different specificities of the two receptors. Our findings suggest that M-factor communication plays an important role in defining the species, whereas P-factor communication is able to undergo a certain degree of flexible adaptation–perhaps as a first step toward prezygotic isolation in S. pombe.

show abstract

Section: Introductionmentioning

confidence: 99%

Asymmetric diversification of mating pheromones in fission yeast

2019

View full text Add to dashboard Cite

show abstract

“…Sex pheromones can be produced de novo in specialized glands or tissues. For instance, most male lizards have femoral glands secreting both lipophilic and proteinaceous compounds, while the sex peptide pheromones of female newts are secreted by abdominal glands (see e.g., Martín & López, 2010;Nakada et al, 2017). Odour communication seems to play an important role for reproduction also in birds, with uropygial glands producing oily secretions likely used for sex recognition in most bird species (Caro et al, 2015).…”

Section: Sex Pheromones: Chemical Nature and Biosynthesismentioning

confidence: 99%

Does climate warming influence sexual chemical signaling?

Groot

Zizzari

2019

Animal Biol.

View full text Add to dashboard Cite

Global climate is changing at a rapid pace and the pivotal question is if the rate and extent of species’ responses to stressful events enable them to persist in a changing world. Although the consequences of rapid environmental changes on animal life-history traits are receiving considerable attention, our understanding of how temperature fluctuations affect sexual chemical communication in animals is scarce. Male-female interactions often depend on pheromone detectability and sudden shifts in environmental temperature are expected to disrupt communication between potential mates. Whether organisms can adapt to temperature-induced changes at both signaller and receiver levels is virtually unexplored. In this perspective paper, we first provide a broad overview of the sex pheromone pathway, from biosynthesis to detection, and outline the importance of chemical-based mate choice. Finally, through several study cases, we highlight how thermal stress may interfere with chemical communication between the sexes, and discuss the potential evolutionary consequence of temperature stress.

show abstract

“…Newts have other unique biological properties such as large genome sizes (Elewa et al, 2017), tumor-resistance (Ingram, 1972;Okamoto, 1987), physiological polyspermy in their fertilization (Picheral, 1977;Charbonneau et al, 1983;Iwao, 2012), and sexual pheromones for mating-behavior (Nakada et al, 2017). Furthermore, Mouchet and other groups showed that newts are useful animals for toxicity testing of chemical compounds (Mouchet et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Advanced microinjection protocol for gene manipulation using the model newt Pleurodeles waltl

Hayashi¹,

Nakajima²,

Kyakuno³

et al. 2019

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Urodele amphibian newts have an outstanding history as experimental animals in various research fields such as developmental biology and regeneration biology. We have reported a model experimental system using the Spanish newt, Pleurodeles waltl, and it enables reverse/ molecular genetics through gene manipulation. Microinjection is one of the core techniques in gene manipulation in newts. In the present study, we examined the conditions of the microinjection method, such as egg preparation, de-jelly solution, and formulation of injection medium. We have successfully optimized the injection protocol for P. waltl newts, and our improved protocol is more efficient and lower in cost than previous methods. This protocol can be used for the microinjection of plasmid DNA with I-SceI or mRNA, as well as genome editing using the CRISPR-Cas9 system. This protocol will facilitate research through gene manipulation in newts.

show abstract

Imorin: a sexual attractiveness pheromone in female red-bellied newts (Cynops pyrrhogaster)

Cited by 26 publications

References 36 publications

Asymmetric diversification of mating pheromones in fission yeast

Asymmetric diversification of mating pheromones in fission yeast

Does climate warming influence sexual chemical signaling?

Advanced microinjection protocol for gene manipulation using the model newt Pleurodeles waltl

Contact Info

Product

Resources

About