Immunotoxicological effects of streptozotocin and alloxan: In vitro and in vivo studies

Diab, Randa; Fares, Mohammad M.; Abedi‐Valugerdi, Manuchehr; Kumagai‐Braesch, Makiko; Holgersson, Jan; Hassan, Manal M.

doi:10.1016/j.imlet.2014.12.006

Cited by 23 publications

(27 citation statements)

References 20 publications

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“…It has been reported that supplementation of fish oil can prevent the development of alloxan-induced diabetes mellitus in experimental animals. 40 HbA1c reflects a large amount of blood glucose attached to hemoglobin in red blood cells to form glycosylated hemoglobin, and thus is an important factor to monitor long-term blood glucose balance. The effects of individual polyunsaturated fatty acids on preventing alloxan induced diabetes mellitus in experimental animals were studied.…”

Section: Discussionmentioning

confidence: 99%

Anti-diabetic activity of peony seed oil, a new resource food in STZ-induced diabetic mice

Wang

et al. 2015

Food Funct.

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This study was conducted to investigate the components of a new resource food in China, peony seed oil (PSO) by GC-MS (gas chromatography-mass spectrometry), its inhibitory effects on carbohydrate hydrolyzing enzymes in vitro and its anti-diabetic effects on mice induced by streptozotocin (STZ). The results showed that peony seed oil showed weak anti-α-amylase activity; however, strong anti-α-glucosidase activity was noted. The GC-MS analysis of the oil showed 9 constituents of which α-linolenic acid was found to be the major component (38.66%), followed by linoleic acid (26.34%) and oleic acid (23.65%). The anti-diabetic potential of peony seed oil was tested in STZ induced diabetic mice. Administration of peony seed oil and glibenclamide reduced the blood glucose level and the area under curve (AUC) in STZ induced diabetic mice. There were significant increases in body weight, liver glycogen content, serum insulin level, high-density lipoprotein cholesterol (HDL-C) and decreases in glycosylated hemoglobin (HbA1C), total serum cholesterol (TC), and triglyceride (TG) in test groups as compared to the untreated diabetic groups. In vivo antioxidant studies on STZ induced diabetic mice revealed the reduction of malondialdehyde (MDA) and increase of glutathione peroxides (GSH-px), superoxide dismutase (SOD), and glutathione (GSH). The results provided a sound rationale for future clinical trials of oral administration of peony seed oil to alleviate postprandial hyperglycemia in streptozotocin-induced diabetic mice.

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Section: Discussionmentioning

confidence: 99%

Anti-diabetic activity of peony seed oil, a new resource food in STZ-induced diabetic mice

Wang

et al. 2015

Food Funct.

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“…b). GLUT2 is further expressed in the kidney and liver; known side effects in this model include kidney and liver damage, reduced animal body weight, and increased risk of developing tumors . STZ also affects the immune system, leading to early lymphopenia in vitro and in vivo with an unexpected toxicity against CD8 T cells and B cells.…”

Section: Autoimmune‐component In Type 1 Diabetes In Humans In Nonobementioning

confidence: 99%

“…These properties of STZ may even exert a protective effect against the onset of diabetes in young NOD mice, potentially by inducing a regulatory T‐cell population . Further, since STZ exhibits immunosuppressive effects, islet grafts may survive longer in STZ‐induced diabetic mice compared with mice injected with other diabetes‐inducing drugs . STZ‐induced diabetic mice represent an economic strategy to induce diabetes, since the maintenance of NOD mice under SPF conditions is associated with relatively high costs.…”

Section: Autoimmune‐component In Type 1 Diabetes In Humans In Nonobementioning

confidence: 99%

Immunological aspects of allogeneic pancreatic islet transplantation: a comparison between mouse and human

et al. 2019

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These authors equally contributed to the work. SUMMARYPancreatic islet allotransplantation is a treatment for patients with severe forms of type 1 diabetes. As long-term graft function and survival are not yet optimal, additional studies are warranted in order to continue improving transplant outcomes. The mechanisms of islet graft loss and tolerance induction are often studied in murine diabetes models. Despite numerous islet transplantation studies successfully performed over recent years, translation from experimental mouse models to human clinical application remains elusive. This review aims at critically discussing the strengths and limitations of current mouse models of diabetes and experimental islet transplantation. In particular, we will analyze the causes leading to diabetes and compare the immunological mechanisms responsible for rejection between mouse and human. A better understanding of the experimental mouse models should facilitate translation to human clinical application. Transplant International 2019; 32: 903-912

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“…Streptozotocin (STZ) (2-deoxy-2-(3-(methyl-3-nitrosoureido)-D-glucopyranose) is a nitrosourea compound that targets beta cells by entering via the glucose transporter, GLUT2, and is a widely used drug for inducing T1DM by inducing stress and DNA alkylation (cleaving) leading to cellular apoptosis [65,66]. STZ can either be administered intraperitoneally at a low dose (40-60 mg/g in 0.1M citrate buffer) continuously for 5 days or at a single high dosage (100-200 mg/kg), though the osteoporosis phenotype is dosage dependent [64].…”

Section: Type 1 Diabetic Mellitus Animal Modelsmentioning

confidence: 99%

Determining the Role of Sost and Sostdc1 During Fracture Healing

Yee¹

2016

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Immunotoxicological effects of streptozotocin and alloxan: In vitro and in vivo studies

Cited by 23 publications

References 20 publications

Anti-diabetic activity of peony seed oil, a new resource food in STZ-induced diabetic mice

Anti-diabetic activity of peony seed oil, a new resource food in STZ-induced diabetic mice

Immunological aspects of allogeneic pancreatic islet transplantation: a comparison between mouse and human

Determining the Role of Sost and Sostdc1 During Fracture Healing

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