Immunotoxicity and transcriptome analysis of zebrafish embryos in response to glufosinate-ammonium exposure

Xiong, Gaofeng; Deng, Yunyun; Li, Jiali; Cao, Zigang; Liao, Xinjun; Liu, Yi; Lu, Huiqiang

doi:10.1016/j.chemosphere.2019.124423

Cited by 54 publications

(16 citation statements)

References 58 publications

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“…In general, the first few bases were prone to recognition errors during sequencing, and these few bases were just the size of the random primers during the sequencing process. Thus, the recognition errors in the first few bases were speculated to be caused by incomplete pairing of random primers with RNA templates [39][40][41]. Therefore, quality control of the data generated by sequencing must be performed to obtain high-quality sequencing data for subsequent bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of differential gene expression in the longissimus dorsi muscle from Debao and landrace pigs based on RNA-sequencing

Song

Zeng

et al. 2019

Bioscience Reports

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RNA-seq analysis was used to identify differentially expressed genes (DEGs) at the genetic level in the longissimus dorsi muscle from two pigs to investigate the genetic mechanisms underlying the difference in meat quality between Debao pigs and Landrace pigs. Then, these DEGs underwent functional annotation, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein–protein interaction (PPI) analyses. Finally, the expression levels of specific DEGs were assessed using qRT-PCR. The reference genome showed gene dosage detection of all samples which showed that the total reference genome comprised 22342 coding genes, including 14743 known and 190 unknown genes. For detection of the Debao pig genome, we obtained 14168 genes, including 13994 known and 174 unknown genes. For detection of the Landrace pig genome, we obtained 14404 genes, including 14223 known and 181 unknown genes. GO analysis and KEGG signaling pathway analysis show that DEGs are significantly related to metabolic regulation, amino acid metabolism, muscular tissue, muscle structure development etc. We identified key genes in these processes, such as FOS, EGR2, and IL6, by PPI network analysis. qRT-PCR confirmed the differential expression of six selected DEGs in both pig breeds. In conclusion, the present study revealed key genes and related signaling pathways that influence the difference in pork quality between these breeds and could provide a theoretical basis for improving pork quality in future genetic thremmatology.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of differential gene expression in the longissimus dorsi muscle from Debao and landrace pigs based on RNA-sequencing

Song

Zeng

et al. 2019

Bioscience Reports

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“…The previous study showed that the alteration of oxidative stress played an important role in glufosinate-induced early life toxicity in zebrafish as characterized by transcriptome analysis. 12 Thus, the correspondent higher antioxidant responses at both protein and mRNA levels in the Rac-glufosinate group suggested that the discrepancy in oxidative stress following Rac-and L-glufosinate exposures may explain the differences in early life toxicity of zebrafish.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Assessing the Efficacy-Risk of the Widely Used Chiral Glufosinate: Switch from the Racemate to the Single Enantiomer?

Yue

Kong

Shen

et al. 2020

Environ. Sci. Technol. Lett.

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The chirality of pesticides has emerged as an important issue in pesticide design and applications because enantiomers can differ greatly in their environmental fate and risk. Despite the increasing concern over the unintended effects of inactive or less effective enantiomers, massive amounts of chiral pesticides are applied as racemic mixtures. As a potential substitute for glyphosate, the market of glufosinate is expected to grow rapidly. However, very little information is available on its enantioselective efficacy-risk assessment, especially at the environmentally relevant concentration. The overall aim of this study is to determine if the switch from glufosinate racemic mixture to l-glufosinate was favored regarding environmental health. Thus, we examined (i) the herbicidal activity of Rac- and l-glufosinate to the weed, Echinochloa crus-galli, and (ii) adverse effects on nontarget zebrafish (Danio rerio). Significant higher herbicidal activities were achieved by l-glufosinate at both 1 and 5 mg/L. Embryotoxicity and teratogenicity are significantly different: Rac-glufosinate induced a significantly higher lethality (LC50 = 17.29 mg/L) and malformations. The oxidative stress markers, including superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA), were preferably induced by Rac-glufosinate at mRNA and enzymatic activity levels. In summary, we suggest that l-glufosinate should be applied preferably over Rac-glufosinate.

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“…Glufosinato, Sal de Amônio. Genotoxicidade (Xiong et al, 2019), hepatotoxicidade, desregulação endócrina .…”

Section: Fomesafenunclassified

Agrotóxicos: críticas à regulação que permite o envenenamento do país

Hess

Nodari

Lopes-Ferreira

2021

Desenvolv. Meio Ambiente

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O artigo contém uma análise das autorizações para a comercialização e o consumo de agrotóxicos no país na última década, bem como os seus efeitos na saúde humana e no meio ambiente. No período entre 2010 e 2018, a quantidade de agrotóxicos comercializados no Brasil aumentou mais do que o dobro do que cresceu a área cultivada no país. A permissividade da gestão de agrotóxicos no Brasil é ilustrada pelo fato de que, do total de ingredientes ativos químicos com registro no país em julho de 2020, 121 (30%) não tinham uso permitido na União Europeia (EU). A razão da proibição do uso na EU dos produtos autorizados no Brasil, no período referido, está associada aos efeitos adversos a humanos e a outros organismos resultantes da exposição aos ingredientes químicos de agrotóxicos, já descritos na literatura científica. Soja, milho, algodão e cana-de-açúcar constam entre as culturas que tiveram o maior número de novos agrotóxicos com uso autorizado no Brasil entre janeiro de 2019 e junho de 2020. Outra falta de rigor na gestão de agrotóxicos é ilustrada pela deriva, que causa danos agudos e crônicos a terceiros. Adicionalmente, a fragilidade da norma brasileira refere-se ao estabelecimento de um valor de dose, abaixo da qual eles seriam seguros. Contudo, resultados experimentais de estudos mostraram, por exemplo, que os agrotóxicos à base de glifosato, melation e piriproxifem causaram a morte de embriões de peixes em apenas 24 horas de exposição, independentemente da concentração utilizada, desafiando assim a hipótese de que existe dose segura para a exposição a tais produtos. Dessa forma, é urgente não só aumentar o rigor científico dos estudos sobre o tema, mas também promover a participação pública nas tomadas de decisão, garantindo o acesso a todas as informações contidas nos dossiês que acompanham as solicitações de registro e de autorização dos agrotóxicos.

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Immunotoxicity and transcriptome analysis of zebrafish embryos in response to glufosinate-ammonium exposure

Cited by 54 publications

References 58 publications

Transcriptome analysis of differential gene expression in the longissimus dorsi muscle from Debao and landrace pigs based on RNA-sequencing

Transcriptome analysis of differential gene expression in the longissimus dorsi muscle from Debao and landrace pigs based on RNA-sequencing

Assessing the Efficacy-Risk of the Widely Used Chiral Glufosinate: Switch from the Racemate to the Single Enantiomer?

Agrotóxicos: críticas à regulação que permite o envenenamento do país

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