Immunotherapy for pythiosis: Effect on NTPDase activity in lymphocytes of an experimental model

Bach, Barbara Charlotte; Leal, Daniela Bitencourt Rosa; Ruchel, Jader Betsch; Souza, Viviane do Carmo Gonçalves; Maboni, Grazieli; Pozzo, Marcelo Dal; Schlemmer, Karine Bizzi; Alves, Sydney Hartz; Santúrio, Jânio Morais

doi:10.1016/j.biopha.2010.09.016

Cited by 12 publications

(14 citation statements)

References 29 publications

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“…Our data also showed that the mixture of these P. insidiosum proteins seems to trigger a Th1 cytokine profile in successfully PiV-immunized mice challenged with L. major . The Leishmania mouse model confirmed previous reports [2, 4, 8, 9, 10, 11, 12, 14, 15, 16, 23, 24, 25] indicating that the mixture of P. insidiosum proteins was sufficient to stimulate an immunomodulatory response in the immunized mice challenged with L. major .…”

Section: Discussionsupporting

confidence: 88%

“…These data are also supported by recent studies in experimentally infected rabbits with P. insidiosum [12, 15, 16, 24]. The latter studies showed that during rabbit experimental pythiosis an increase in E-NTPDase (an enzyme that is present in lymphocytes and is involved in the immune response) and the decrease in ATP or ADP in sera could be involved in the binding of P2Y receptors expressed on the surface of lymphocytes, which in turn could activate a Th2 response [15]. The ecto-adenosine deaminase (E-ADA) present in low concentration in a typical Th2 inflammation increased after immunotherapy, substantiating the hypothesis that during immunotherapy a switching from Th2 to Th1 subset occurs in successfully treated hosts [16].…”

Section: Discussionsupporting

confidence: 87%

“…It was later found in successfully treated mammals that in addition to the switching from an eosinophilic to a mononuclear cell response, a shift from a Th2 to a Th1 cytokine profile occurred [2]. Moreover, when this hypothesis was tested in the experimental rabbit model, a similar profile was found with the expression of lymphocytic NTPDase [15] and ecto-adenosine deaminase [16], also pointing to a Th2 down-regulation.…”

Section: Introductionmentioning

confidence: 87%

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A Leishmania murine model to evaluate the immunomodulatory properties of Pythium insidiosum proteins

Inêz-Ferreira

Mendoza

Vilela

et al. 2017

Heliyon

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Pythium insidiosum immunomodulatory vaccine (PiV) has been tested in clinical and experimental pythiosis. Previous data showed that P. insidiosum immunogens have the ability to switch the Th2 immune response, normally in place during pythiosis, to a curative Th1 response. Pythiosis cannot be reproduced in experimental rodents with the exception of rabbits, and thus thorough evaluation of PiV´s immunomodulatory properties has been limited by the lack of a compatible inbred mouse model. In this study, we took advantage of the murine BALB/c Leishmania infection model, where infected mice produce a Th2 response, to evaluate the PiV Th2 to Th1 immunomodulatory potential. Twenty-one days following challenge with L. major, large cutaneous granulomas developed in control mice, consistent with the expected Th2 response. In contrast, Leishmania-induced cutaneous lesions in PiV-immunized mice were minimal or absent. Flow cytometry analysis of spleen cells from mice immunized with PiV and subsequently challenged with L. major displayed more CD4+ and CD8+ cells than the control group. Moreover, spleen cells from mice that were immunized with PiV then challenged with L. major secreted high levels of IFN-γ, with a moderate IL-2, IL-4, and IL-10 mixed cytokine profile upon in vitro re-stimulation with PiV. Anti-P. insidiosum IgG1 in immunized animals was present at low titers suggesting a minor immunological role for this Ig isotype in this model. Our preliminary data showed that BALB/c mice challenged with L. major represent an attractive model in which to study PiV´s immunomodulatory properties.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 87%

See 1 more Smart Citation

A Leishmania murine model to evaluate the immunomodulatory properties of Pythium insidiosum proteins

Inêz-Ferreira

Mendoza

Vilela

et al. 2017

Heliyon

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“…The activity of the enzymes E-NTPDase and E-ADA has been related with the immune response in fungal diseases as pythiosis (Bach et al, 2010) and sporotrichosis (Castro et al, 2012). The results obtained in our experiment showed that during the experimental infection by C. neoformans occurred a decrease in the E-NTPDase activity when used ATP and ADP substrates during the infection.…”

Section: Discussionsupporting

confidence: 52%

E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans

Azevedo

Ferreiro

Silva

et al. 2014

Veterinary Microbiology

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“…rice paddies, irrigation channels and reservoirs, are all potential resources of this emerging pathogen 10 . Currently, there are neither effective antimicrobials nor vaccines against pythiosis 8,[11][12][13][14] . Unlike fungi, P. insidiosum as an oomycete lacks ergosterol in the cell membrane which is the target of most antifungal drugs 8,15 .…”

Section: Introductionmentioning

confidence: 99%

Detection of diketopiperazine and pyrrolnitrin, compounds with anti-Pythium insidiosum activity, in a Pseudomonas stutzeri environmental strain

Thongsri¹,

Aromdee²,

Yenjai³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aims. Screening of bacterial flora for strains producing metabolites with inhibitory effects on the human pathogenic oomycete Pythium insidiosum. Separation and characterization of extracts from Pseudomonas stutzeri with anti-Pythium inhibitory activity. Search for genes with anti-Pythium effect within the genome of P. stutzeri. Methods. A total of 88 bacterial strains were isolated from water resources in northeastern Thailand. Two screening methods were used to establish their inhibitory effects on P. insidiosum. One strain, P. stutzeri ST1302 was randomly chosen, and the extract with anti-P. insidiosum activity was fractionated and subfractionated using liquid column chromatography and purified by thin layer chromatography. The chemical structure of purified fractions was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectrometry. Further, search for genes involved in the anti-Pythium activity (phenazine-1-carboxylic acid, 2,4-diacetylphloroglucinol, pyoluteorin and pyrrolnitrin) was undertaken in this P. stutzeri strain using primers described in the literature. Results. Anti-P. insidiosum activity was detected in 16 isolates (18.2%). In P. stutzeri ST1302, a subfraction labeled PYK7 exhibited strong activity against this oomycete. It was assigned to the diketopiperazines as cyclo(D-Pro-L-Val). In the search for genes, one gene region was successfully amplified. This corresponded to pyrrolnitrin. The results suggest the possibility of using the related metabolites against P. insidiosum. This is the first report on the inhibitory effects of P. stutzeri against this oomycete. The results may contribute to the development of antimicrobial drugs/probiotics against pythiosis.

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Immunotherapy for pythiosis: Effect on NTPDase activity in lymphocytes of an experimental model

Cited by 12 publications

References 29 publications

A Leishmania murine model to evaluate the immunomodulatory properties of Pythium insidiosum proteins

A Leishmania murine model to evaluate the immunomodulatory properties of Pythium insidiosum proteins

E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans

Detection of diketopiperazine and pyrrolnitrin, compounds with anti-Pythium insidiosum activity, in a Pseudomonas stutzeri environmental strain

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