Immunotargeting of Nanocrystals by SpyCatcher Conjugation of Engineered Antibodies

Pedroso, Cássio Cardoso Santos; Mann, Victor R.; Zuberbühler, Kathrin; Bohn, Markus-Frederik; Yu, Jessica; Altoé, Virginia; Craik, Charles S.; Cohen, Bruce E.

doi:10.1021/acsnano.1c07856

Cited by 21 publications

(22 citation statements)

References 77 publications

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“…Unlike other inorganic molecular marker such as quantum dots, these lanthanide-based UCNPs do not contain elements known to cause toxicity, and previous animal injections have been safely demonstrated in [38,47,55], opening the door to clinical translation. In addition to that, they are also able to be conjugated to antibodies enabling molecular targeting to cells, providing cellular level contrast and selectivity [51]. Table 1 includes a comparison of the two main types of time-resolved biomedical sensors: systems requiring optics [35,56] and their optics-free counterparts [47,57].…”

Section: Discussionmentioning

confidence: 99%

“…Synthesis of Core/Shell aUCNPs β-phase NaEr 0.8 Yb 0.2 F 4 aUCNPs were synthesized with minor modifications of the previously described methodology [38,51]. The ErCl 3 •xH 2 O (0.32 mmol, 122 mg), YbCl 3 •6H 2 O (0.08 mmol, 31 mg), oleic acid (OA, 3.25 g), and 1-octadececene (ODE, 4 mL) precursors were added into a 50-mL three-neck flask with subsequent heating to 110 °C under vacuum.…”

Section: Nanoparticle Synthesismentioning

confidence: 99%

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Fully Integrated Ultra-thin Intraoperative Micro-imager for Cancer Detection Using Upconverting Nanoparticles

et al. 2022

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Purpose Intraoperative detection and removal of microscopic residual disease (MRD) remain critical to the outcome of cancer surgeries. Today’s minimally invasive surgical procedures require miniaturization and surgical integration of highly sensitive imagers to seamlessly integrate into the modern clinical workflow. However, current intraoperative imagers remain cumbersome and still heavily dependent on large lenses and rigid filters, precluding further miniaturization and integration into surgical tools. Procedures We have successfully engineered a chip-scale intraoperative micro-imager array—without optical filters or lenses—integrated with lanthanide-based alloyed upconverting nanoparticles (aUCNPs) to achieve tissue imaging using a single micro-chip. This imaging platform is able to leverage the unique optical properties of aUCNPs (long luminescent lifetime, high-efficiency upconversion, no photobleaching) by utilizing a time-resolved imaging method to acquire images using a 36-by-80-pixel, 2.3 mm $$\times$$ × 4.8 mm silicon-based electronic imager micro-chip, that is, less than 100-µm thin. Each pixel incorporates a novel architecture enabling automated background measurement and cancellation. We have validated the performance, spatial resolution, and the background cancellation scheme of the imaging platform, using resolution test targets and mouse prostate tumor sample intratumorally injected with aUCNPs. To demonstrate the ability to image MRD, or tumor margins, we evaluated the imaging platform in visualizing a single-cell thin section of the injected prostate tumor sample. Results Tested on USAF resolution targets, the imager is able to achieve a resolution of 71 µm. We have also demonstrated successful background cancellation, achieving a signal-to-background ratio of 8 when performing ex vivo imaging on aUCNP-injected prostate tumor sample, improved from originally 0.4. The performance of the imaging platform on single-cell layer sections was also evaluated and the sensor achieved a signal-to-background ratio of 4.3 in resolving cell clusters with sizes as low as 200 cells. Conclusion The imaging system proposed here is a scalable chip-scale ultra-thin alternative for bulky conventional intraoperative imagers. Its novel pixel architecture and background correction scheme enable visualization of microscopic-scale residual disease while remaining completely free of lenses and filters, achieving an ultra-miniaturized form factor—critical for intraoperative settings.

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Section: Discussionmentioning

confidence: 99%

Section: Nanoparticle Synthesismentioning

confidence: 99%

Fully Integrated Ultra-thin Intraoperative Micro-imager for Cancer Detection Using Upconverting Nanoparticles

et al. 2022

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Section: Modification Strategies For Qdsmentioning

confidence: 99%

“…Pedroso et al reported a method for the preparation of Antibody-QDs using the binding ability between SpyCatcher and SpyTag. [136] The premise of this method is to attach SpyCatcher to the surface of QDs using covalent conjugation, as shown in Figure 5a. Succinimidyl ester-maleimide SM(PEG) 2 cross-linker introduces thiol-reactive groups to the surface of the nanoparticles, and then SM(PEG) 2 reacts with primary amines to form a stable maleimide intermediate, which can react with the single-Cys SpyCatcher proteins.…”

Section: Functionalizing With Biomoleculesmentioning

confidence: 99%

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Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Piao

Yang

Cui

et al. 2022

Adv Funct Materials

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With the rapid progress in nanomaterials and biochemistry, there has been an explosion of interest in biomolecule-modified quantum dots (QDs) for biomedical applications. Metal chalcogenide quantum dots (MCQDs), as the most widely studied QDs, have attracted tremendous attention in the biomedical field on account of their unique and excellent optical properties and the ease of biomolecular modifications. Herein, important advances in MCQDs over recent years are reviewed, from materials design to biomedical applications. Especially, this review focuses on the challenges encountered in the applications of MCQDs in biomedical fields and how these problems can be solved by rational design of synthesis methods and modifications, which have opened a universal route to develop the functionalized MCQDs. Moreover, recent processes in bioimaging, biosensing, and cancer therapy based on MCQDs are examined, including the rapid detection and diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This review provides broad insights into MCQDs in the biomedical field and will inspire material researchers to develop MCQDs in the future.

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Ballistic Ejection of Microdroplets from Overpacked Interfacial Assemblies

Bordia

Streubel

et al. 2023

Adv Funct Materials

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Spontaneous emulsification, resulting from the assembly and accumulation of surfactants at liquid–liquid interfaces, is an interfacial instability where microdroplets are generated and diffusively spread from the interface until complete emulsification. Here, it is shown that an external magnetic field can modulate the assembly of paramagnetic nanoparticle surfactants (NPSs) at liquid–liquid interfaces to trigger an oversaturation in the areal density of the NPSs at the interface, as evidenced by a marked reduction in the interfacial tension, γ, and corroborated with a magnetostatic continuum theory. Despite the significant reduction in γ, the presence of the magnetic field does not cause stable interfaces to become unstable. Upon rapid removal of the field, however, an explosive ejection of a plume of microdroplets from the surface occurs, a dynamical interfacial instability which is termed explosive emulsification. This explosive event rapidly reduces the areal density of the NPSs to its pre‐field level, stabilizing the interface. The ability to externally suppress or trigger the explosive emulsification and controlled generation of tens of thousands of microdroplets, uncovers an efficient energy storage and release process, that has potential applications for controlled and directed delivery of chemicals and remotely controlled soft microrobots, taking advantage of the ferromagnetic nature of the microdroplets.

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Immunotargeting of Nanocrystals by SpyCatcher Conjugation of Engineered Antibodies

Cited by 21 publications

References 77 publications

Fully Integrated Ultra-thin Intraoperative Micro-imager for Cancer Detection Using Upconverting Nanoparticles

Fully Integrated Ultra-thin Intraoperative Micro-imager for Cancer Detection Using Upconverting Nanoparticles

Recent Advances in Metal Chalcogenide Quantum Dots: From Material Design to Biomedical Applications

Ballistic Ejection of Microdroplets from Overpacked Interfacial Assemblies

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