Immunotargeted Photodynamic Therapy for Cholesteatoma

Gluth, Michael B.; Kaufmann, Yihong; Dornhoffer, John L.; Ferguson, Scott

doi:10.1097/mao.0000000000000590

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…The nanocapsules were coated with an antibody against epidermal growth factor receptor (EGFR) to selectively target the keratinocytes. In vitro results showed selective targeting and killing of keratinocytes, but not mucosal cells after activation of the nanocapsules with infrared light ( Gluth et al, 2015 ).…”

Section: Novel Drug Delivery Systemsmentioning

confidence: 99%

Overcoming barriers: a review on innovations in drug delivery to the middle and inner ear

Delaney,

Liew,

Lye

et al. 2023

Front. Pharmacol.

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Despite significant advances in the development of therapeutics for hearing loss, drug delivery to the middle and inner ear remains a challenge. As conventional oral or intravascular administration are ineffective due to poor bioavailability and impermeability of the blood-labyrinth-barrier, localized delivery is becoming a preferable approach for certain drugs. Even then, localized delivery to the ear precludes continual drug delivery due to the invasive and potentially traumatic procedures required to access the middle and inner ear. To address this, the preclinical development of controlled release therapeutics and drug delivery devices have greatly advanced, with some now showing promise clinically. This review will discuss the existing challenges in drug development for treating the most prevalent and damaging hearing disorders, in particular otitis media, perforation of the tympanic membrane, cholesteatoma and sensorineural hearing loss. We will then address novel developments in drug delivery that address these including novel controlled release therapeutics such as hydrogel and nanotechnology and finally, novel device delivery approaches such as microfluidic systems and cochlear prosthesis-mediated delivery. The aim of this review is to investigate how drugs can reach the middle and inner ear more efficiently and how recent innovations could be applied in aiding drug delivery in certain pathologic contexts.

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Section: Novel Drug Delivery Systemsmentioning

confidence: 99%

Overcoming barriers: a review on innovations in drug delivery to the middle and inner ear

Delaney,

Liew,

Lye

et al. 2023

Front. Pharmacol.

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“…The reported photosensitizers are mainly porphyrin-based derivatives ( Lucky et al, 2015 , Ormond and Freeman, 2013 ). Several classes of non-porphyrin derivatives, such as cyanines ( Fekrazad et al, 2015 , Gluth et al, 2015 ), xanthenes ( Gianotti et al, 2014 , Wang et al, 2013 , Yao et al, 2014 ), phenothiazines ( Samy et al, 2015 , Yu et al, 2015 ), and anthraquinones ( Sharma and Davids, 2012 , Theodossiou et al, 2009 ) were investigated recently. Most of the reported photosensitizers absorb light in the visible region.…”

Section: Introductionmentioning

confidence: 99%

Dicyanomethylene Substituted Benzothiazole Squaraines: The Efficiency of Photodynamic Therapy In Vitro and In Vivo

Wei

Shen

et al. 2017

EBioMedicine

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The lack of ideal photosensitizers limits the clinicalapplication of photodynamic therapy (PDT). Here we report the PDT efficiency of dicyanomethylene substituted benzothiazole squaraine derivatives. This class of squaraine derivatives possess strong absorption and long excitation and emission wavelengths (ex/em, 685/720 nm). They show negligible dark toxicity, but can generate singlet oxygen under irradiation resulting in the apoptosis and necrosis of cells (phototoxicity). Changing the side chains of these compounds greatly influences their albumin-binding rate, cellular uptake and their phototoxicity. One of the squaraine derivatives with two methyl butyrate side chains shows high PDT efficiency in a mouse subcutaneous xenograft model under the irradiation of a 690 nm laser. These results show the great potential of dicyanomethylene substituted benzothiazole squaraines to be the leading compound of near-infrared photosensitizers in PDT.

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“…Several methods, studied both in vivo and in vitro, have been proposed to inhibit or slow the growth of cholesteatoma (5)(6)(7)(8)(9)(10)(11). Residual cholesteatoma, caused by incomplete surgical removal of squamous epithelium from the middle ear, has been a well-known challenge (12)(13)(14).…”

mentioning

confidence: 99%

The Viability and Growth of HaCaT Cells After Exposure to Bioactive Glass S53P4-Containing Cell Culture Media

Sarin

Vuorenmaa

Vallittu

et al. 2021

Otology &Amp; Neurotology

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Hypothesis: Bioactive glass (BG) S53P4 reduces the viability of epidermal keratinocyte-derived immortalized cell line, HaCaT in sufficient concentrations in vitro. Background: Although used in mastoid obliteration surgery, there is no data available on whether BG S53P4 granules have an inhibitory or excitatory effect on keratinocytes, found in normal skin and ear cholesteatoma in vivo. Methods: HaCaT cell cultures were incubated with a direct BG S53P4 granule contact. Microscopic evaluation of the cultures was performed and interleukin-6 (IL-6) and -8 (IL-8) concentrations were measured from the medium samples. In addition, BG granules were incubated in two cell culture media for 6 days and the pure media were used in confluent HaCaT cultures preceding cell viability assay. Finally, a scratch assay test was performed to reveal the possible BG effect on HaCaT cultures.Results: Eight to ten cell thick layers of dead HaCaT cells were noticed after a 2-day BG granule contact. With a BG concentration of 2.5%, IL-6 and IL-8 concentrations were smaller compared with the control group without BG after 2 days' incubation. Overall, HaCaT cell viability decreased when BG was incubated in keratinocyte growth medium, but did not change in Dulbecco's modified Eagle's medium. In a scratch assay test, cell regrowth in the scratch area was notable in cultures without BG. Conclusions: BG S53P4 seems to have an inhibitory effect on HaCaT cell growth. Although further studies are needed, this observation seems advantageous for cholesteatoma treatment.

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Immunotargeted Photodynamic Therapy for Cholesteatoma

Cited by 10 publications

References 21 publications

Overcoming barriers: a review on innovations in drug delivery to the middle and inner ear

Overcoming barriers: a review on innovations in drug delivery to the middle and inner ear

Dicyanomethylene Substituted Benzothiazole Squaraines: The Efficiency of Photodynamic Therapy In Vitro and In Vivo

The Viability and Growth of HaCaT Cells After Exposure to Bioactive Glass S53P4-Containing Cell Culture Media

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