Immunosuppressive proteins secreted by the gastrointestinal nematode parasite Heligmosomoides polygyrus

Pritchard, David; Lawrence, Catherine E.; Appleby, P.; Gibb, I.A.; Glover, Kevin A.

doi:10.1016/0020-7519(94)90140-6

Cited by 31 publications

(25 citation statements)

References 24 publications

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“…There has consequently been great interest in the potential of Excretory-Secretory molecules from this parasite ("HES") to down-modulate pathology in vivo 17,18 . Indeed, protective effects are seen following treatment of mice with HES products 19 through pathways which are now being identified 18,20 .…”

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confidence: 99%

Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products

Johnston

Robertson

Harcus

et al. 2015

JoVE

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Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

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confidence: 99%

Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products

Johnston

Robertson

Harcus

et al. 2015

JoVE

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“…For example, the nematode Heligmosomoides polygyrus contains immunomodulatory factors capable of either stimulating (1,2) or inhibiting (3,4) T and B cell responses. With respect to Ascaris suum, we and others have shown that infection with the nematode or the use of its soluble products or extracts at different stages of purification potentiates or suppresses the immune responses (5)(6)(7).…”

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Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum

Oshiro

Rafael

Enobe

et al. 2004

Braz J Med Biol Res

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The extract of Ascaris suum suppresses the humoral and cellular immune responses to unrelated antigens in the mouse. In order to further characterize the suppressive components of A. suum, we produced specific monoclonal antibodies which can provide an important tool for the identification of these proteins. The A. suum immunosuppressive fractions isolated by gel filtration from an extract of adult worms were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells and the cloned hybrid cells obtained were screened to determine the specificity of secreted antibodies. Three monoclonal antibodies named MAIP-1, MAIP-2 and MAIP-3 were selected and were shown to react with different epitopes of high molecular weight proteins from the A. suum extract.

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“…Highly effective immune evasion mechanisms are key elements of the long-lasting persistence of parasitic worms. Both parasite-specific and nonspecific immune suppression are well documented (19,47,60). Usually, acute infection stimulates antigen-specific T-cell proliferation, but with increasing exposure to parasite antigens, the immune system becomes hyporesponsive (34).…”

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confidence: 99%

Functional Analysis of Effector and Regulatory T Cells in a Parasitic Nematode Infection

et al. 2008

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Immunosuppressive proteins secreted by the gastrointestinal nematode parasite Heligmosomoides polygyrus

Cited by 31 publications

References 24 publications

Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products

Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products

Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum

Functional Analysis of Effector and Regulatory T Cells in a Parasitic Nematode Infection

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