Immunosuppressive effects of Centipeda periodontii: selective cytotoxicity for lymphocytes and monocytes

Shenker, Bruce J.; Berthold, Peter; Dougherty, P. A.; Porter, K.

doi:10.1128/iai.55.10.2332-2340.1987

Cited by 14 publications

(5 citation statements)

References 49 publications

(37 reference statements)

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“…Products of Corvnehacterium parvum, Actinomyces viscosus and oral spirochetes such as Treponema denticota affect macrophages, provoking the release of mediators such as hydrogen peroxide and prostaglandin that are capable of inhibiting lytnphocyte function (7,14,15). Other bacteria produce toxins which selectively kill itnmune cells (1,12). Preliminary data suggest that B. intermedius inhibit itntnunological responses during the early stages (within first 24 h) of cell activation.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of Bacteroides products on in vitro human lymphocyte functions

Shenker

Slots

1989

Oral Microbiology and Immunology

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Bacteroides spp. have been implicated in the pathogenesis of several diseases, including periodontal diseases. In this study sonic extracts of 6 Bacteroides spp. were examined for their abilities to alter human lymphocyte function. We found that soluble extracts from Bacteroides intermedius, Bacteroides endodontalis, Bacteroides asaccharolyticus, Bacteroides melaninogenicus, and to a lesser degree Bacteroides loescheii, caused dose-dependent inhibition of human lymphocyte responsiveness to both mitogens and antigens. Suppression involved altered DNA, RNA and protein synthesis as well as immunoglobulin production. In contrast, Bacteroides gingivalis did not suppress these responses; instead, it stimulated lymphocyte proliferation and enhanced immunoglobulin production. It has been proposed that impaired host defense may play a pivotal role in the pathogenesis of many infections. The data presented in this paper suggest that microbial mediated immunosuppression may conceivably alter the nature and consequences of host-parasite interactions in periodontal disease.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of Bacteroides products on in vitro human lymphocyte functions

Shenker

Slots

1989

Oral Microbiology and Immunology

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“…Shenker et al reported that soluble sonic extracts of several C. periodontii strains possessed the activities causing immunogenic dysfunction or immunosuppression, which may make it easy for bacteria to infect and colonized gingival tissues, and may contribute to the initiation and progression of the early stages of periodontal disease. (24,25) In this study, it has been revealed that the endotoxic activities of C. periodontii LPS are comparable to those of E. coli LPS. Although the pathogenic mechanisms of periodontopathic organisms are complex and require further study, the data here suggest that C. periodontii LPS may be one possible pathogenic factor relating this bacterium to periodontal disease.…”

Section: Resultsmentioning

confidence: 61%

Isolation and characterization of lipopolysaccharide from Centipeda periodontii ATCC 35019

Kokeguchi

Tsutsui

Kato

et al. 1990

Oral Microbiology and Immunology

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Lipopolysaccharide (LPS) was extracted from cells of Centipeda periodontii ATCC 35019 by a hot phenol-water method, purified by nuclease treatment and by repeated ultracentrifugations. The LPS was composed of 21.7% hexose, 9.1% hexosamine, 10.3% fatty acid, 0.5% protein, 2.5% phosphorus, 4.3% 2-keto-3-deoxyoctonate(KDO) and 2.3% heptose. The major fatty acids of the LPS were 3OH C13:0 and C13:0. C. periodontii LPS was able to gelatinize Limulus amebocyte and to produce the local Schwartzman reaction in rabbits. LPS from C. periodontii exhibited strong immunobiological activities, mitogenicity on splenocytes of C3H/HeN mice, and stimulation of interleukin 1 (IL-1) production in peritoneal macrophages of C3H/HeN mice.

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“…While the nature and contribution of the immune system to the pathogenesis of periodontal disease are poorly understood, recent studies suggest that the development of these disorders may be associated with immunologic dysfunction (reviewed in reference 16). Although the basis for this dysfunction is unclear, we have found that several putative periodontal pathogens are capable of inhibiting human lymphocyte responsiveness (17)(18)(19)(20)(21). The ability of microorganisms to suppress the immune response of the host may affect the course of initial infection by facilitating spread, multiplication, and persistence and may lead to enhanced susceptibility to infection by secondary pathogens (reviewed in references 15 and 28).…”

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confidence: 93%

Immunosuppressive effects of Prevotella intermedia on in vitro human lymphocyte activation

1991

Self Cite

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In this study, we have assessed four strains of Prevotella intermedia, isolated from periodontally involved lesions, for their ability to inhibit lymphocyte functions. All four strains were found to cause a dose-dependent inhibition of B-and T-cell proliferation in response to mitogens and antigens. This was reflected in altered DNA, RNA, and protein syntheses. Furthermore, P. intermedia appeared to affect the early stages of cell activation. This was ascertained by kinetic analysis in which it was determined that the extract had to be present during the first 24 h of incubation to cause suppression. Moreover, direct assessment of the early stages of cell activation indicated that release of cytokines and expression of the interleukin 2 receptor and CD69 on T cells were inhibited by P. intermedia sonic extracts. Finally, preliminary characterization of the immunosuppressive agent indicates that it has a molecular mass of approximately 50 kDa and is heat labile. It has been proposed that impaired host defense may play a pivotal role in the pathogenesis of many infections. The data presented in this paper suggest that microbially mediated immunosuppression may contribute to the pathogenesis of periodontal disease by altering the nature and consequences of host-parasite interactions. * Corresponding author. CO2 for 2 to 3 days at 37°C. The medium used was 3.7% brain heart infusion broth (Difco Laboratories, Detroit, Mich.) supplemented with 0.0005% hemin, 0.00005% menadione, 0.5% yeast extract, and 0.05% L-cysteine hydrochlo-

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Immunosuppressive effects of Centipeda periodontii: selective cytotoxicity for lymphocytes and monocytes

Cited by 14 publications

References 49 publications

Immunomodulatory effects of Bacteroides products on in vitro human lymphocyte functions

Immunomodulatory effects of Bacteroides products on in vitro human lymphocyte functions

Isolation and characterization of lipopolysaccharide from Centipeda periodontii ATCC 35019

Immunosuppressive effects of Prevotella intermedia on in vitro human lymphocyte activation

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