Immunosuppressive effect of pregnant mouse serum on allostimulatory activity of dendritic cells

Shojaeian, Jaleh; Moazzeni, Seyed Mohammad; Nikoo, Shohreh; Bozorgmehr, Mahmood; Nikougoftar, Mahin; Zarnani, Amir Hassan

doi:10.1016/j.jri.2007.02.006

Cited by 15 publications

(15 citation statements)

References 36 publications

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“…As we described elsewhere, the balance of myeloid and lymphoid DCs is finely tuned throughout pregnancy thus pointing to an eminent immunoregulatory role of DCs in maintenance of pregnancy [42]. Furthermore, in our recent studies we found that microenvironment of the feto-maternal interface and pregnancy milieu could instruct dendritic cells to protect semiallogenic fetus through inhibition of antigen presentation and induction of Th2 cytokine profile [43,44]. This pregnancy-induced preprogrammed property of neonatal DCs can also play an important role in low incidence of GVHD following cord blood transplantation.…”

Section: Discussionsupporting

confidence: 61%

“…This conflict can be explained by the differences in the used methods, as in Goriely et al study, DCs were generated through in vitro culture of blood monocytes in a medium containing GM-CSF and IL-4 and these DCs were under influence of in vitro microenvironment, and so could not reflect the in vivo conditions completely. In fact, recently we observed the potency of pregnancy-related factors to suppress the allostimulatory function of DCs [43]. Moreover, the method used by Goriely et al, produces only myeloid DCs while in our study we used non-manipulated CBDCs and because of saving both DC subsets more reliable judgment could be made about their impact on T cell differentiation and what really happens in GVHD.…”

Section: Discussionmentioning

confidence: 77%

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Cord blood dendritic cells prevent the differentiation of naïve T-helper cells towards Th1 irrespective of their subtype

2008

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Allogeneic cord blood transplantation is associated with a less severe graft-versus-host disease (GVHD). This observation is thought to be due to immaturity of cord blood cell immune capabilities. Dendritic cells (DCs) are the most potent antigen-presenting cells of the immune system capable of initiation and regulation of immune responses. In this investigation, we hypothesized that nonmanipulated cord blood dendritic cells (CBDCs) not only differ in their functional maturity from adult peripheral blood DCs (PBDCs) but also differ in their subsets and their preference in promoting Th1 or Th2 immune responses. Non-manipulated fresh DCs were isolated from cord blood (CB) and adult peripheral blood (PB) mononuclear cells as lineage marker negative cells. The differences in expression of costimulatory molecules, the proportion of myeloid and lymphoid DCs subsets, their immunostimulatory characteristics and their influence on promoting the differentiation of naïve T cells towards Th1 or Th2 cells were then investigated in these two populations. Our results showed that freshly isolated CBDCs, similar to cord blood monocyte derived DCs, were poor inducers of IFN-c secretion while they increased the induction of IL-4 production by T cells in comparison with PBDCs. CBDCs were also poor stimulators of allogenic T cells in mixed leukocyte reaction compared to adult peripheral blood dendritic cells. They also displayed decreased expression of HLA-DR and CD86 molecules. The ratio of lymphoid DCs (CD11c -, CD123 ? ) to myeloid DCs (CD11c ? , CD123 -) was significantly higher in CB compared to PB. We conclude that CBDCs preferential priming of naive T cells towards Th2 population, seems to be an intrinsic property independent of their subtype. This property along with their functional immaturity should contribute to outcome of cord blood transplantation.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 77%

Cord blood dendritic cells prevent the differentiation of naïve T-helper cells towards Th1 irrespective of their subtype

2008

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“…Dendritic cells (DCs) have also been implicated in maintaining maternal immune tolerance to the developing fetus [26] because of their ability to influence the differentiation of T cells to a T helper type 2 (Th2) subset [27] and their cross-talk with the most abundant leukocyte population at the maternofetal interface, uterine natural killer (uNK) cells [28]. Therefore, we investigated whether DCs could respond to PSG treatment.…”

Section: Resultsmentioning

confidence: 99%

“…The important players in placentation include macrophages [11], DCs [26], uNK cells [30], endothelial cells, and trophoblasts [39]. Previous work with murine and human PSGs identified monocytes/macrophages as the target cells for protein binding and activity [5, 17].…”

Section: Discussionmentioning

confidence: 99%

Murine Pregnancy-Specific Glycoprotein 23 Induces the Proangiogenic Factors Transforming-Growth Factor Beta 1 and Vascular Endothelial Growth Factor A in Cell Types Involved in Vascular Remodeling in Pregnancy1

Johnson

Chen

et al. 2008

Biology of Reproduction

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Haemochorial placentation is a unique physiological process in which the fetal trophoblast cells remodel the maternal decidual spiral arteries to establish the fetoplacental blood supply. Pregnancy-specific glycoproteins (PSGs) are members of the carcinoembryonic antigen family. PSGs are produced by the placenta of rodents and primates and are secreted into the bloodstream. PSG23 is one of 17 members of the murine PSG family (designated PSG16 to PSG32). Previous studies determined that PSGs have immunoregulatory functions due to their ability to modulate macrophage cytokine secretion. Here we show that recombinant PSG23 induces transforming growth factor (TGF) beta, TGFB1, and vascular endothelial growth factor A (VEGFA) in primary murine macrophages and the macrophage cell line RAW 264.7 cells. In addition, we identified new cell types that responded to PSG23 treatment. Dendritic cells, endothelial cells, and trophoblasts, which are involved in maternal vasculature remodeling during pregnancy, secreted TGFB1 and VEGFA in response to PSG23. PSG23 showed cross-reactivity with human cells, including human monocytes and the trophoblast cell line, HTR-8/SVneo cells. We analyzed the binding of PSG23 to the tetraspanin CD9, the receptor for PSG17, and found that CD9 is not essential for PSG23 binding and activity in macrophages. Overall these studies show that PSGs can modulate the secretion of important proangiogenic factors, TGFB1 and VEGFA, by different cell types involved in the development of the placenta.

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“…Our results showed that treatment with vitamin D3 of WEC from patients with URSA lowered the IFN-g:IL-10 ratio, as well as suggesting its potential therapeutic effect on controlling immune-mediated pregnancy losses. As we have shown earlier, DCs are the sentinel antigen-presenting cells residing in both nonpregnant (18) and pregnant endometrium (17) and that their function is under tight control of the uterus microenvironment (16,35). Given the high density of VDR present in endometrial DCs (4), vitamin D3 could be considered as a major regulator of this cell type.…”

Section: Discussionmentioning

confidence: 99%

Effects of 1,25(OH)2 vitamin D3 on cytokine production by endometrial cells of women with recurrent spontaneous abortion

Tavakoli

Jeddi-Tehrani

Salek-Moghaddam

et al. 2011

Fertility and Sterility

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Immunosuppressive effect of pregnant mouse serum on allostimulatory activity of dendritic cells

Cited by 15 publications

References 36 publications

Cord blood dendritic cells prevent the differentiation of naïve T-helper cells towards Th1 irrespective of their subtype

Cord blood dendritic cells prevent the differentiation of naïve T-helper cells towards Th1 irrespective of their subtype

Murine Pregnancy-Specific Glycoprotein 23 Induces the Proangiogenic Factors Transforming-Growth Factor Beta 1 and Vascular Endothelial Growth Factor A in Cell Types Involved in Vascular Remodeling in Pregnancy1

Effects of 1,25(OH)2 vitamin D3 on cytokine production by endometrial cells of women with recurrent spontaneous abortion

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