Immunosuppressive and Hematopoiesis-Supporting Properties of Stromal Cells at Low Oxygen

Abstract

+ cells decreased after co-culture with both stromal cells in 20% O 2 . This effect was revealed only for MMSC at 5% O 2 . After 72 hours in co-culture with MMSCs, mononuclear cells from umbilical cord blood (CBMCs) maintained the same number of colony forming units (CFU) as compared with the initial CBMC suspension. The number of CFU was 30% higher at 5% O 2 than in 20% O 2 (p < 0.05). The CFU number in CBMCs after co-culture with fFBs decreased and a stimulatory effect of low oxygen was not observed. CBMCs that were adhered to MMSCs formed colonies of undifferentiated progenitors after 2 weeks of co-culture both at 20% and 5% O 2 . Colonies were not found on fFBs. The immunomodulatory potency and the hematopoiesis-supportive capacity of stromal cells can vary depending on their tissue origin and may be modified by the partial pressure of oxygen.

Human Adipose-Tissue Derived Stromal Cells in Combination with Hypoxia Effectively Support Ex Vivo Expansion of Cord Blood Haematopoietic Progenitors

“…Despite the fact that such feature of haematopoietic niche as low O 2 is well recognised and is considered to be one of the most important mechanisms of haematopoiesis regulation, ex vivo hypoxic protocols are used quite rarely [ 1 , 23 , 24 , 37 , 38 ]. Nevertheless, most of them demonstrated a positive effect of hypoxia on ex vivo haematopoiesis despite the considerable differences in the study design.…”