Immunosuppressive activity and alpha interferon concentrations in human embryo culture media as an index of potential for successful implantation

Jones, K. P.; Warnock, SH; Urry, RL; Edwin, Sam; Mitchell, MD

doi:10.1016/0020-7292(92)90696-g

Cited by 3 publications

(4 citation statements)

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“…The embryo has been shown to secrete immune-suppressive compounds in culture that have long remained undefined, 17 or found not to be novel. 19 Other compounds, such as hCG, have been identified in maternal circulation (albeit only several days post implantation), but they do not reflect pregnancy viability, only its existence, and they persist in circulation even after pregnancy ends. hCG rescues the corpus luteum following implantation, alters endometrial morphology, and may have immune-suppressive effects through specific binding sites (CG/LH-R).…”

Section: Unique Circumstances Require Unique Compounds: the Preimplanmentioning

confidence: 99%

Applying Embryo‐Derived Immune Tolerance to the Treatment of Immune Disorders

Barnea

2007

Annals of the New York Academy of Sciences

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Pregnancy is a unique immune state in which both mother and embryo/fetus tolerate and interact with one another through term, without interfering with the mother's native immunity. When the embryo is viable, it initiates maternal immune tolerance (IT)--ut not immune suppression. The balance is complex: some immune disorders are ameliorated during pregnancy, despite the presence of an "inflammatory" environment. We have identified a pregnancy viability biomarker--preimplantation factor (PIF)--secreted only by viable embryos, which helps to initiate this maternal tolerance and uterine receptivity. Using anti-PIF antibodies, we have detected PIF in the fetus and placenta. Beyond enhancing uterine receptivity locally, PIF has also been detected in maternal circulation, and may promote peripheral tolerance. In the fetus, PIF may help avoid a "graft-versus-host"-type reaction PIF exhibits unique potent immune-modulatory effects and its synthetic analogue has been shown to exert significant protection in diverse immune scenarios. Nontoxic, low-dose, short-term PIF administration has led to long-term effects in preclinical models of multiple sclerosis (MS), juvenile diabetes mellitus (JDM), and graft-versus-host disease (GVHD), in a manner enabling its translation into a clinical setting. Further investigation of this compound is warranted.

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Section: Unique Circumstances Require Unique Compounds: the Preimplanmentioning

confidence: 99%

Applying Embryo‐Derived Immune Tolerance to the Treatment of Immune Disorders

Barnea

2007

Annals of the New York Academy of Sciences

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“…Another approach that has been adopted in ART is the targeted metabolomics (78), where only one or few known metabolites are quantified in the culture medium. A number of target biomarkers have been investigated and related to embryo development, such as human chorionic gonadotropin (hCG) (79), Apolipoprotein A1 (80), human leukocyte antigen-G (81,82), granulocyte-macrophage colony-stimulating factor (83), pregnancy-specific beta-1 glycoprotein (84), alpha interferone 2 (85), and platelet activating factor (86). In a recent work, using electrochemical impedance spectroscopy, hCGb, IL-8, and tumor necrosis factor a (TNFa) were detected from culture media of single human embryos: nonviable embryos produced higher levels of IL-8 and TNFa; interestingly, hCGb levels were able to discriminate between morphologically identical viable embryos (68).…”

Section: Measuring Metabolic Endpointsmentioning

confidence: 99%

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

Casciani¹,

Galliano

Franasiak

et al. 2021

F&S Reviews

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For decades, assisted reproductive technology (ART) procedures have been performed manually thanks to the meticulous work of skilled embryologists. Recently, new technologies have been developed with three main scopes: improving embryo culture conditions; making diagnostic evaluations more consistent and reliable; and allowing ART procedures to become progressively less subjective and operatordependent. This review aimed to answer the following questions: is automation likely to be successfully incorporated into the in vitro fertilization laboratory and clinical ART in the future? If so, would such automation result in improved outcomes in ART?An electronic search of PubMed was performed to identify articles in English language that addressed automation in ART. Studies were classified in decreasing categories: randomized controlled trials; prospective controlled trials; prospective noncontrolled trials; retrospective studies; and experimental studies. Research and development data from investigators were included.There are a number of separate platforms that have been developed until now to address different parts of the ART process: gradual change of embryo culture medium; noninvasive embryo monitoring with time-lapse or metabolome analysis; and automated vitrification. It is conceivable that future automation enhancements in the in vitro fertilization laboratory may improve consistency and throughput and reduce the risk of human error associated with the performance of repetitive tasks. Nevertheless, a need remains for a platform able to integrate all separate technologies, capable of successfully interconnecting them in a way that assures continued chain of custody for the gametes and embryos. Moreover, controlled trials will be fundamental to demonstrate the usefulness of automation in ART. (Fertil Steril Rev Ò 2021;2:251-64. Ó2021 by American Society for Reproductive Medicine.

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“…59,60 The earliest studies on the human embryo secretome revealed that conditioned media contained bioactive immunoregulatory molecules, and that this activity may be associated with implantation success. [61][62][63][64] One of these molecules was identified as PAF (1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine), which is one of the most well-characterized embryo-secreted autotrophic molecules, but is not used clinically as either a biomarker or a culture media additive. 65 Another immunologically active molecule that has been extensively investigated is soluble human leukocyte antigen G (sHLA-G), after early studies found that the presence of sHLA-G in conditioned media was predictive of clinical pregnancy.…”

Section: Secretomementioning

confidence: 99%

“…In addition to metabolites, the proteins which the embryo releases into the surrounding medium (the secretome) can also somewhat reflect developmental ability 59 60 The earliest studies on the human embryo secretome revealed that conditioned media contained bioactive immunoregulatory molecules, and that this activity may be associated with implantation success 61 64 One of these molecules was identified as PAF (1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine), which is one of the most well-characterized embryo-secreted autotrophic molecules, but is not used clinically as either a biomarker or a culture media additive 65 .…”

Section: Assessment Of Human Embryo Phenotypementioning

confidence: 99%

Impact of the IVF laboratory environment on human preimplantation embryo phenotype

Gardner

Kelley

2017

J Dev Orig Health Dis

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The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its morphology, developmental kinetics, physiology and metabolism, can be affected by numerous components of the laboratory and embryo culture system (which comprise the laboratory environment). The culture media formulation is important in determining embryo phenotype, but this exists within a culture system that includes oxygen, temperature, pH and whether an embryo is cultured individually or in a group, all of which can influence embryo development. Significantly, exposure of an embryo to one suboptimal component of the culture system of laboratory typically predisposes the embryo to become more vulnerable to a second stressor, as has been well documented for atmospheric oxygen and individual culture, as well as for oxygen and ammonium. Furthermore, the inherent viability of the human embryo is derived from the quality of the gametes from which it is created. Patient age, aetiology, genetics, lifestyle (as well as ovarian stimulation in women) are all known to affect the developmental potential of gametes and hence the embryo. Thus, as well as considering the impact of the IVF laboratory environment, one needs to be aware of the status of the infertile couple, as this impacts how their gametes and embryos will respond to an in vitro environment. Although far from straight forward, analysing the interactions that exist between the human embryo and its environment will facilitate the creation of more effective and safer treatments for the infertile couple.

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Immunosuppressive activity and alpha interferon concentrations in human embryo culture media as an index of potential for successful implantation

Cited by 3 publications

References 0 publications

Applying Embryo‐Derived Immune Tolerance to the Treatment of Immune Disorders

Applying Embryo‐Derived Immune Tolerance to the Treatment of Immune Disorders

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

Impact of the IVF laboratory environment on human preimplantation embryo phenotype

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